LOCUS       sequence-299800-       11033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for targeting the Replace reporter to the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11033)
  AUTHORS   Danner E, Lebedin M, de la Rosa K, Kuhn R
  TITLE     A homology independent sequence replacement strategy in human cells 
            using a CRISPR nuclease.
  JOURNAL   Open Biol. 2021 Jan;11(1):200283. doi: 10.1098/rsob.200283. Epub 
            2021 Jan 27.
  PUBMED    33499763
REFERENCE   2  (bases 1 to 11033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Open 
            Biol."; date: "2021-01"; volume: "11(1):200283. doi"; pages: " 
            10.1098/rsob.200283. Epub 2021 Jan 27"
COMMENT     Sequence Label: pAAVS1-NEO-CAG-BFP-pA-GFP-pA
FEATURES             Location/Qualifiers
     source          1..11033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    843..868
                     /label=SA
                     /note="splice acceptor site"
     CDS             892..945
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             961..1764
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     enhancer        2106..2409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2411..2688
                     /label=chicken beta-actin promoter
     intron          2689..3705
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3629..3651
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3713..3732
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3795..3804
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3801..3803,3804..3806,3807..4520)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4545..4562)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4551..4662
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4786..4795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4792..4794,4795..4797,4798..5511)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5600..5619)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5665..5720
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5719..5738)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6081..6097)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6105..6121
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6129..6135,6136..6153,6154..6159))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6174..6195
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6269..7105
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(7139..7158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7140..7158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7165..7182)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7165..7181)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7174..7196)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7319..7621
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7500..7519
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(join(9018..9809,9810..9878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9641..9660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10002..10590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10491..10510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10744..10761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10878..10899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10914..10919,10920..10937,10938..10944)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10952..10968
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10976..10992
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11013..11031
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagc
      841 ttctgacctc ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga
      901 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct cgagacgcgt
      961 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1021 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1081 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1141 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1201 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1261 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1321 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1381 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1441 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1501 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1561 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1621 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1681 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1741 cgccttcttg acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct
     1801 attaaacaat aaagatgtcc actaaaatgg aagtwtytmy wgwymtmyyk tgkkawsatt
     1861 gtttttctct tgattcccac tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt
     1921 tcatagcctg aagaacgaga tcagcagcct ctgttccaca tacacttcat tctcagtatt
     1981 gttttgccaa gttctaattc catcagaagc tgggagctcc accgcggtgg cggccgctct
     2041 agaactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     2101 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     2161 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     2221 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     2281 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     2341 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     2401 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     2461 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     2521 gggggggggg ggcgcgcgcc rgggggggsg gggsggggsg rggggsgggg sggggcgagg
     2581 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     2641 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     2701 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     2761 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     2821 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     2881 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     2941 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     3001 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     3061 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     3121 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     3181 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     3241 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     3301 gccggggrgg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     3361 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     3421 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     3481 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     3541 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     3601 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     3661 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     3721 ctggttattg tgctgtctca tcattttggc aaagaattcc gctccatctt ctagaaagac
     3781 tggagtgcga tcgcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     3841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg
     3901 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     3961 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc
     4021 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4081 tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc gccgaggtga
     4141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg
     4201 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca
     4261 tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacgtggagg
     4321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4381 tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     4441 agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actctcggca
     4501 tggacgagct gtacaagtga gaattcctag agctcgctga tcagcctcga ctgtgccttc
     4561 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     4621 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4681 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagagaa
     4741 tagcaggcat gcctccatct tctagaaaga ctggagttta attaagccac catggtgagc
     4801 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     4861 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     4921 accctgaagc tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     4981 accctgggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     5041 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     5101 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     5161 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     5221 tacaactaca acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag
     5281 gccaacttca agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac
     5341 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     5401 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     5461 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta acgcgtgaat
     5521 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     5581 ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     5641 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     5701 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     5761 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     5821 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     5881 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     5941 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     6001 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc
     6061 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6121 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6181 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6241 tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca gaaaagcccc
     6301 atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca cctcctgtta
     6361 ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc tccttgccag
     6421 aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag agcttggcag
     6481 ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc accctgcgct
     6541 accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc actgatcctg
     6601 gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac aaaatcagaa
     6661 taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa tttatattgt
     6721 tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg tcctggcagg
     6781 gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat ggtgcgtcct
     6841 aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc cttctttcct
     6901 taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg tcccgcttcc
     6961 ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga gaggcgctca
     7021 ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc tgccccttcc
     7081 ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat tcaattcgcc
     7141 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7201 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7261 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca
     7321 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     7381 tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     7441 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     7501 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     7561 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     7621 aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc
     7681 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     7741 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     7801 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     7861 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg
     7921 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     7981 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8041 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8101 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
     8161 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8221 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8281 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8341 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     8401 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8461 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
     8521 cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     8581 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     8641 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     8701 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     8761 tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     8821 gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8881 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc aaaaaggatc
     8941 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9001 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9061 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9241 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9301 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9361 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9421 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9481 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9541 tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg agaatagtgt
     9601 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9841 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9901 tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga gttttcgttc
     9961 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10021 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10081 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10141 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10201 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10261 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10321 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10381 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10441 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10501 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10561 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10621 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10681 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10741 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10801 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10861 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10921 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10981 aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag gga
//
