LOCUS       sequence-299802-        8069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Dec;14(12):2580-96. doi: 10.1261/rna.1351608. Epub 2008 
            Oct 31.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1351608"; journalName: "RNA"; date: "2008-12"; volume: 
            "14"; issue: "12"; pages: "2580-96"
COMMENT     Sequence Label: pDESTmycDDX17
FEATURES             Location/Qualifiers
     source          1..8069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2942..2966)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2998..3017)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2999..3017)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3028..3045)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3028..3044)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3037..3059)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    3449..3583
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3486..3505)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3540..3559
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3709..4164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3796..3815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4006..4027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4281..4638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4281..4472
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4304..4324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4489..4624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4689..5483
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4743..4762)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5353..5372
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5547..5595
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5636..5655)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5755..5777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5901..6005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6006..6074,6075..6866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6224..6243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7037..7625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7526..7545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7779..7796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat gcccaccggc tttgtagccc cgattctctg tgttttgctc ccgtctccga
      781 cgagagaggc ggcgacggtg gcgtctgcga cgggagacag cgcgtcggag cgagagagcg
      841 ctgcgcctgc cgccgcccca acagcggagg cgccgccgcc atcggtcgtc accagaccgg
      901 agccgcaggc cctcccgagc ccggccatcc gtgccccgct cccagatctc tatccttttg
      961 ggaccatgcg cggaggaggc tttggggacc gggaccggga tcgtgaccgt ggaggatttg
     1021 gagcaagagg tggtggtggc cttcccccga agaaatttgg taatcctggg gagcgtttgc
     1081 gtaaaaaaaa gtgggatttg agtgagctcc ccaagtttga gaaaaatttt tatgtggaac
     1141 atccggaagt agcaaggctg acaccatatg aggttgatga gctacgccga aagaaggaga
     1201 ttacagtgag ggggggagat gtttgtccta aacccgtgtt tgccttccat catgctaact
     1261 tcccacaata tgtaatggat gtgttgatgg atcagcactt tacagaacca actccaattc
     1321 agtgccaggg atttccgttg gctcttagtg gccgggatat ggtgggcatt gctcagactg
     1381 gctctgggaa gacgttggcg tatctcctgc ctgcaattgt tcatattaac caccagccat
     1441 acttggaaag gggagatggc ccaatctgtc tagttctggc tcctaccaga gagcttgccc
     1501 agcaagtaca gcaggtggcc gatgactatg gcaaatgttc tagattgaag agtacttgta
     1561 tttatggagg tgctcctaaa ggtccccaga ttcgagactt ggaaagaggt gttgagatct
     1621 gcatagccac tcctggacgt ctgatagatt tcctggagtc aggaaagaca aatcttcgcc
     1681 gatgtactta ccttgtattg gacgaagctg acagaatgct tgatatgggg tttgaacccc
     1741 agatccgtaa aattgttgac caaatcaggc ctgataggca gacactgatg tggagtgcaa
     1801 cctggccaaa agaagtaaga cagcttgcag aggatttcct tcgtgattac acccagatca
     1861 acgtaggcaa tctggagttg agtgccaacc acaacatcct ccagatagtg gatgtctgca
     1921 tggaaagtga aaaagaccac aagttgatcc aactaatgga agaaataatg gctgaaaagg
     1981 aaaacaaaac aataatattt gtggagacaa agagacgctg tgatgatctg actcgaagga
     2041 tgcgcagaga tggttggcca gctatgtgta tccatggaga caagagtcaa ccagaaagag
     2101 attgggtact taatgagttc cgttctggaa aggcacccat ccttattgct acagatgtag
     2161 cctcccgtgg gctagatgtg gaagatgtca agtttgtgat caactatgac tatccaaaca
     2221 gctcagagga ttatgtgcac cgtattggcc gaacagcccg tagcaccaac aagggtaccg
     2281 cctatacctt cttcacccca gggaacctaa aacaggccag agagcttatc aaagtgctgg
     2341 aagaggccaa tcaggctatc aatccaaaac tgatgcagct tgtggaccac agaggaggcg
     2401 gcggaggcgg gggtggtcgt tctcgttacc ggaccacttc ttcagccaac aatcccaatc
     2461 tgatgtatca ggatgagtgt gaccgaaggc ttcgaggagt caaggatggt ggccggagag
     2521 actctgcaag ctatcgggat cgtagtgaaa ccgatagagc tggttatgct aatggcagtg
     2581 gctatggaag tccaaattct gcctttggag cacaagcagg ccaatacacc tatggtcaag
     2641 gcacctatgg ggcagctgct tatggcacca gtagctatac agctcaagaa tatggtgctg
     2701 gcacttatgg agctagtagc accacctcaa ctgggagaag ttcacagagc tctagccagc
     2761 agtttagtgg gataggccgg tctgggcagc agccacagcc actgatgtca caacagtttg
     2821 cacagcctcc gggagctacc aatatgatag gttacatggg gcagactgcc taccaatacc
     2881 ctcctcctcc tccccctcct cctccttcac gtaaatgagc ggccgcactc gagatatcta
     2941 gacccagctt tcttgtacaa agtggttgat cgcgtgcatg cgacgtcata gctctctccc
     3001 tatagtgagt cgtattataa gctaggcact ggccgtcgtt ttacaacgtc gtgactggga
     3061 aaactgctag cttgggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     3121 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     3181 tgttaaacta gctgcatatg cttgctgctt gagagttttg cttactgagt atgatttatg
     3241 aaaatattat acacaggagc tagtgattct aattgtttgt gtattttaga ttcacagtcc
     3301 caaggctcat ttcaggcccc tcagtcctca cagtctgttc atgatcataa tcagccatac
     3361 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3421 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3481 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3541 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcgatcctg cattaatgaa
     3601 tcggccaacg cgcggggaga ggcggtttgc gtattggctg gcgtaatagc gaagaggccc
     3661 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     3721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     3781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     3841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     3901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     3961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4141 acaaaatatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     4201 tatttcacac cgcatacgcg gatctgcgca gcaccatggc ctgaaataac ctctgaaaga
     4261 ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4561 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4621 ggcctaggct tttgcaaaaa gcttgattct tctgacacaa cagtctcgaa cttaaggcta
     4681 gagccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4741 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4801 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4861 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4921 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4981 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5041 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5101 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5161 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5221 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5281 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5341 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5401 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5461 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5521 gcccaacctg ccatcacgat ggccgcaata aaatatcttt attttcatta catctgtgtg
     5581 ttggtttttt gtgtgaatcg atagcgataa ggatccgcgt atggtgcact ctcagtacaa
     5641 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5701 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5761 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5821 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5881 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5941 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6001 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6061 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6121 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6181 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     6241 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6301 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6361 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6421 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6481 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6541 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6601 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6661 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6721 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6781 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6841 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6901 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6961 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7021 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7081 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7141 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7201 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7261 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7321 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7381 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7441 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7501 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7561 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7621 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7681 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     7741 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     7801 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7861 gagcttgcaa ttcgcgcttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8041 aaataggcgt attacgaggc cctttcacg
//
