LOCUS       sequence-299824-       11375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomally expresses KLF2 and NANOG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, 
            Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, 
            Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H
  TITLE     Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts
            of Multiple Mammalian Species.
  JOURNAL   Stem Cell Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002.
  PUBMED    33798453
REFERENCE   2  (bases 1 to 11375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2021 Mar 23. pii: S2213-6711(21)00132-6. doi: 
            10.1016/j.stemcr.2021.03.002."
COMMENT     Sequence Label: pCE-K2N
FEATURES             Location/Qualifiers
     source          1..11375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3..22)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    68..123
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(122..141)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(484..500)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(484..500)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(497..519)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    508..524
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(532..538,539..556,557..562))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    577..598
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1077..3002
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      3304..5093
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(5635..5652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5806..6394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5886..5905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6565..7356,7357..7425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7188..7207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7426..7530)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7561..7940
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7942..8218
                     /label=chicken beta-actin promoter
     intron          8219..9235
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     9159..9181
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     9243..9262
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      9290..9299
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10385..10450
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
       61 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
      121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
      181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
      241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
      301 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
      361 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
      421 atagctgtcc ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc
      481 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      541 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      601 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatctcaat
      661 tctcatgttt gacagcttat catcgataag ctgatcctca caggccgcac ccagcttttc
      721 ttccgttgcc ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg
      781 agaatcccca tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga
      841 ggctcaggac gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg
      901 tgatgaggac gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag
      961 cgaccatgaa ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct
     1021 gtattcactg agcgtcgtct aataaagatg tctattgatc tcttttagtg tgaatcatgt
     1081 ctgacgaggg gccaggtaca ggacctggaa atggcctagg agagaaggga gacacatctg
     1141 gaccagaagg ctccggcggc agtggacctc aaagaagagg gggtgataac catggacgag
     1201 gacggggaag aggacgagga cgaggaggcg gaagaccagg agccccgggc ggctcaggat
     1261 cagggccaag acatagagat ggtgtccgga gaccccaaaa acgtccaagt tgcattggct
     1321 gcaaagggac ccacggtgga acaggagcag gagcaggagc gggaggggca ggagcaggag
     1381 gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag
     1441 gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
     1501 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
     1561 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag
     1621 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     1681 caggagcagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag
     1741 gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
     1801 caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag
     1861 gagcaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag
     1921 gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag
     1981 gggcaggagc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg
     2041 caggagcagg aggtggaggc cggggtcgag gaggcagtgg aggccggggt cgaggaggta
     2101 gtggaggccg gggtcgagga ggtagtggag gccgccgggg tagaggacgt gaaagagcca
     2161 gggggggaag tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa aagaggccca
     2221 ggagtcccag tagtcagtca tcatcatccg ggtctccacc gcgcaggccc cctccaggta
     2281 gaaggccatt tttccaccct gtaggggaag ccgattattt tgaataccac caagaaggtg
     2341 gcccagatgg tgagcctgac gtgcccccgg gagcgataga gcagggcccc gcagatgacc
     2401 caggagaagg cccaagcact ggaccccggg gtcagggtga tggaggcagg cgcaaaaaag
     2461 gagggtggtt tggaaagcat cgtggtcaag gaggttccaa cccgaaattt gagaacattg
     2521 cagaaggttt aagagctctc ctggctagga gtcacgtaga aaggactacc gacgaaggaa
     2581 cttgggtcgc cggtgtgttc gtatatggag gtagtaagac ctccctttac aacctaaggc
     2641 gaggaactgc ccttgctatt ccacaatgtc gtcttacacc attgagtcgt ctcccctttg
     2701 gaatggcccc tggacccggc ccacaacctg gcccgctaag ggagtccatt gtctgttatt
     2761 tcatggtctt tttacaaact catatatttg ctgaggtttt gaaggatgcg attaaggacc
     2821 ttgttatgac aaagcccgct cctacctgca atatcagggt gactgtgtgc agctttgacg
     2881 atggagtaga tttgcctccc tggtttccac ctatggtgga aggggctgcc gcggagggtg
     2941 atgacggaga tgacggagat gaaggaggtg atggagatga gggtgaggaa gggcaggagt
     3001 gatgtaactt gttaggagac gccctcaatc gtattaaaag ccgtgtattc ccccgcacta
     3061 aagaataaat ccccagtaga catcatgcgt gctgttggtg tatttctggc catctgtctt
     3121 gtcaccattt tcgtcctccc aacatggggc aattgggcat acccatgttg tcacgtcact
     3181 cagctccgcg ctcaacacct tctcgcgttg gaaaacatta gcgacattta cctggtgagc
     3241 aatcagacat gcgacggctt tagcctggcc tccttaaatt cacctaagaa tgggagcaac
     3301 cagcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
     3361 aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
     3421 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
     3481 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
     3541 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
     3601 agcctatgct acccagatat agattaggat agcatatgct acccagatat agattaggat
     3661 agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat taggatagca
     3721 tatactaccc taatctctat taggatagca tatgctaccc ggatacagat taggatagca
     3781 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
     3841 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
     3901 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
     3961 tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
     4021 ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
     4081 tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
     4141 tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
     4201 ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
     4261 gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
     4321 acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
     4381 gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
     4441 gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
     4501 agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
     4561 atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
     4621 gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
     4681 tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
     4741 ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
     4801 gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
     4861 tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
     4921 ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
     4981 ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
     5041 agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
     5101 aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
     5161 ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
     5221 gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
     5281 atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
     5341 ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
     5401 tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
     5461 gaagggatta catgcactgc cccgcgaaga aggggcagag atgtcgtagt caggtttagt
     5521 tcgtccgggg cggggcatcg atcctctaga gtcgacgcta gcggatccgc tgcattaatg
     5581 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     5641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6241 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6841 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6901 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6961 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7021 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7081 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7141 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7201 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7261 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7321 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7381 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7441 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7501 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgggtc
     7561 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7621 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7681 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7741 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7801 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7861 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7921 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     7981 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
     8041 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     8101 gcgggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     8161 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     8221 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     8281 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     8341 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     8401 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     8461 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     8521 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     8581 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     8641 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     8701 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     8761 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     8821 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     8881 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     8941 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     9001 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     9061 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     9121 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     9181 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     9241 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg ccaccatggc
     9301 gctgagtgaa cccatcctgc cgtccttctc cactttcgcc agcccgtgcc gcgagcgcgg
     9361 cctgcaggag cgctggccgc gcgccgaacc cgagtccggc ggcaccgacg acgacctcaa
     9421 cagcgtgctg gacttcatcc tgtccatggg gctggatggc ctgggcgccg aggccgcccc
     9481 ggagccgccg ccgccgcccc cgccgcctgc gttctattac cccgaacccg gcgcgccccc
     9541 gccctacagc gcccccgcgg gtggcctggt gtctgagctg ctgcgacccg agctggatgc
     9601 gccgccgggg cccgcactgc acggccgctt tctgctggcg ccgcccggcc gcctggtcaa
     9661 ggccgagccc cctgaagcgg acggcggcgg cggctacggc tgcgcccccg ggctgacccg
     9721 tggaccgcgc ggcctcaagc gcgagggcgc cccgggcccg gcggcttcgt gcatgcgagg
     9781 tcccgggggc cgccccccgc cgccgcccga cacaccgccg ctcagccccg acggccccgc
     9841 gcgcctgccc gcgcccggtc cgcgcgcctc cttcccgccg cctttcggtg gccctggttt
     9901 cggcgcgccc gggcccggcc tgcattacgc gccgcctgcg cccccagcct tcggtctctt
     9961 cgacgacgcg gccgccgccg cggcagccct gggcctggcg ccccccgccg cccgcggtct
    10021 cctcacgccg cctgcgtccc cgctggagct gctggaggcc aagccaaagc gcggccgccg
    10081 ctcttggccc cgcaaacgca ccgccactca cacctgcagc tacgcgggct gcggcaagac
    10141 ctacaccaag agttcgcatc tgaaggcgca tctgcgcacg cacacaggtg agaagcccta
    10201 ccactgcaac tgggacggct gcggctggaa gtttgcgcgc tcagacgagc tcacgcgcca
    10261 ctaccgaaag cacacgggcc accggccatt ccagtgccat ctgtgcgatc gtgccttctc
    10321 gcgctccgat cacctggcgc tgcacatgaa acggcacatg gatcctcgcg ctaagcgggc
    10381 accggtgaaa cagactttga attttgacct tctcaagttg gcgggagacg tggagtccaa
    10441 cccagggccg gatatcatga gtgtggatcc agcttgtccc caaagcttgc cttgctttga
    10501 agcatccgac tgtaaagaat cttcacctat gcctgtgatt tgtgggcctg aagaaaacta
    10561 tccatccttg caaatgtctt ctgctgagat gcctcacacg gagactgtct ctcctcttcc
    10621 ctcctccatg gatctgctta ttcaggacag ccctgattct tccaccagtc ccaaaggcaa
    10681 acaacccact tctgcagaga atagtgtcgc aaaaaaggaa gacaaggtcc cagtcaagaa
    10741 acagaagacc agaactgtgt tctcttccac ccagctgtgt gtactcaatg atagatttca
    10801 gagacagaaa tacctcagcc tccagcagat gcaagaactc tccaacatcc tgaacctcag
    10861 ctacaaacag gtgaagacct ggttccagaa ccagagaatg aaatctaaga ggtggcagaa
    10921 aaacaactgg ccgaagaata gcaatggtgt gacgcagaag gcctcagcac ctacctaccc
    10981 cagcctctac tcttcctacc accagggatg cctggtgaac ccgactggga accttccaat
    11041 gtggagcaac cagacctgga acaattcaac ctggagcaac cagacccaga acatccagtc
    11101 ctggagcaac cactcctgga acactcagac ctggtgcacc caatcctgga acaatcaggc
    11161 ctggaacagt cccttctata actgtggaga ggaatctctg cagtcctgca tgcagttcca
    11221 gccaaattct cctgccagtg acttggaggc tgctttggaa gctgctgggg aaggccttaa
    11281 tgtaatacag cagaccacta ggtattttag tactccacaa accatggatt tattcctaaa
    11341 ctactccatg aacatgcaac ctgaagacgt gtgaa
//
