LOCUS       sequence-299827-       10369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expresses iRFP713, Puromycin resistance gene, and 
            rtTAM2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, 
            Shiozawa S, Okano H
  TITLE     Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by 
            ASCL1, microRNA-9/9*, and microRNA-124 Overexpression.
  JOURNAL   Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 
            10.3390/cells10010006.
  PUBMED    33375083
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-12-22"; volume: "10(1). pii: cells10010006. doi"; pages:
            " 10.3390/cells10010006"
COMMENT     Sequence Label: pPB-tetpA(R1R4)-iRPT
FEATURES             Location/Qualifiers
     source          1..10369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    969..1176
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    1187..1310
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1374..1679)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1476..1495)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2021..2680)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2595..2614
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2734..2740,2741..2758,2759..2764))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2789..2913)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2923..3237)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2926..2950)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2997..3015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3068..3086
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3104..3122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3140..3158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3175..3193
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3211..3229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3274..4436
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3505..4427
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4384..4404
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4449..4458
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5403..5456
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5463..6059
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5463..5482)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5959..5979
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6060..6116
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6117..6734,6735..6740,6741..6776,6777..6779,6780..6815,
                     6816..6818,6819..6854,6855..6863)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6913..7501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6966..6986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7384..7395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7785..7906
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7822..7841)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7876..7895
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8095..8157
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8181..8199)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8220..8236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8220..8236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8233..8255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8244..8260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8268..8274,8275..8292,8293..8298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8313..8334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8451..8468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8622..9210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8702..8721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9381..10172,10173..10241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10004..10023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10242..10346)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatctc cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc
     1021 ccaccccacc ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat
     1081 tttattagga aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg
     1141 ggcaaacaac agatggctgg caactagaag gcacagatca agctatcaac tttgtataga
     1201 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1261 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gtcgacctgc
     1321 agactggctg tgtgtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt
     1381 ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc
     1441 acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag
     1501 ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga
     1561 cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt
     1621 acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt
     1681 tcaccagtcc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc
     1741 agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt
     1801 ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga
     1861 tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcacacct ctttttgaca
     1921 tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg
     1981 catactgtta tctggctttt agtaagccgg atccacgcgt ttacgccccg ccctgccact
     2041 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg
     2101 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2161 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2221 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2281 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2341 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2401 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2461 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2521 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2581 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2641 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2701 aaaatctcgc cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg
     2761 taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta
     2821 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     2881 cgtttctcgt tcagcttttt tgtacaaact tgtgatgcta gcggcgatct gacggttcac
     2941 taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacctcgac atacgttctc
     3001 tatcactgat agggagtaaa ctcgacatac gttctctatc actgataggg ataaactcga
     3061 catacgttct ctatcactga tagggagtaa actcgacata cgttctctat cactgatagg
     3121 gagtaaactc gacatacgtt ctctatcact gatagggagt aaactcgaca tcgttctcta
     3181 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgat
     3241 tgaagacgaa agggccgaat tcaagcttcg tgaggctccg gtgcccgtca gtgggcagag
     3301 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     3361 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3421 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3481 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3541 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctcca gtacgtgatt
     3601 cttgatcccg agctggagcc aggggcgggc cttgcgcttt aggagcccct tcgcctcgtg
     3661 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     3721 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     3781 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaggatctg cacactggta
     3841 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     3901 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     3961 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     4021 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctccag
     4081 ggggctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     4141 ggaaaggggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     4201 cgtccaggca cctcgattag ttctggagct tttggagtac gtcgtcttta ggttgggggg
     4261 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     4321 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     4381 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaacac
     4441 gctaccgggc caccatggcc gaagggagcg tggcaagaca gcccgatctg ctgacatgcg
     4501 acgacgaacc aatccacatt ccaggagcta tccagcctca tggactgctg ctggctctgg
     4561 ccgctgacat gactatcgtg gcaggctctg ataacctgcc agagctgacc gggctggcta
     4621 tcggagcact gattggccgg tccgcagccg acgtgttcga ttctgagact cacaatagac
     4681 tgaccatcgc cctggctgaa cctggggctg cagtgggagc cccaattacc gtcggcttca
     4741 caatgcgaaa ggacgctggc tttatcgggt cctggcaccg ccatgatcag ctgatttttc
     4801 tggagctgga accacctcag agagacgtgg cagagccaca ggctttcttt cggagaacaa
     4861 acagcgccat caggcgcctg caggccgctg agactctgga atccgcttgc gcagccgctg
     4921 cacaggaagt gaggaagatc accgggttcg atcgcgtcat gatctacaga ttcgcctcag
     4981 actttagcgg agaagtgatc gcagaagatc gctgtgccga ggtcgaaagt aaactgggcc
     5041 tgcattaccc agcctcaacc gtgcctgcac aggcacgacg gctgtataca atcaaccccg
     5101 tgcggatcat tcccgacatt aattacagac cagtgcccgt cacacctgac ctgaacccag
     5161 tgactgggag gcccatcgat ctgagcttcg ccattctgcg cagcgtgtcc cctgtccacc
     5221 tggagtttat gaggaatatc ggaatgcatg gcaccatgtc tatcagtatt ctgagaggcg
     5281 aaaggctgtg ggggctgatc gtgtgccacc atcggacacc ttactatgtg gacctggacg
     5341 gcaggcaggc ttgtgaactg gtggctcagg tgctggcttg gcagattggc gtgatggaag
     5401 aagagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatgt
     5461 ctatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5521 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5581 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5641 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5701 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5761 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5821 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5881 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5941 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     6001 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg
     6061 ccacgaactt ctctctgtta aagcaagcag gagacgtgga agaaaacccc ggtcctatgt
     6121 ctagactgga caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta
     6181 tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt
     6241 actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca
     6301 ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca
     6361 acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg
     6421 gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc
     6481 agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac
     6541 tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta
     6601 ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag
     6661 ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa
     6721 agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag
     6781 ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg
     6841 accttgacat gctccccggg taactaagta aggatcctct agagttaaca tcgagggatc
     6901 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     7021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     7081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     7141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     7201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     7261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     7321 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     7381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     7441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     7501 catcgatacc gtcgagacct ggaaaaacat ggagcaatca caagtagcaa cacagcagct
     7561 accaatgctg cttgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7621 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct agataactga
     7681 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7741 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7801 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7861 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc tcgagtgcat
     7921 ggatcctttg ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat
     7981 aaacataaat aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa
     8041 cttaatatct attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg
     8101 tcaattttac gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta
     8161 aagagctcca gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg
     8221 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8281 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8341 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8401 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8461 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8521 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8581 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8641 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8701 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8761 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8821 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8881 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8941 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9001 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9061 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9121 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9181 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9241 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9301 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9361 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9421 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9481 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9541 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9601 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9661 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9721 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9781 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9841 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9901 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9961 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10021 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10081 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10141 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10201 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10261 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10321 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
