LOCUS       sequence-299828-        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124.
            Ubiquitously expresses iRFP713, Puromycin resistance gene, and 
            rtTAM2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Nemoto A, Kobayashi R, Yoshimatsu S, Sato Y, Kondo T, Yoo AS, 
            Shiozawa S, Okano H
  TITLE     Direct Neuronal Reprogramming of Common Marmoset Fibroblasts by 
            ASCL1, microRNA-9/9*, and microRNA-124 Overexpression.
  JOURNAL   Cells. 2020 Dec 22;10(1). pii: cells10010006. doi: 
            10.3390/cells10010006.
  PUBMED    33375083
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2020-12-22"; volume: "10(1). pii: cells10010006. doi"; pages:
            " 10.3390/cells10010006"
COMMENT     Sequence Label: pPB-tetpA(hBCL2-miR9/9*-124)-iRPT
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   660..694
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    976..996
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1652..1676
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2415..2439)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2449..2763)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(2452..2476)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2523..2541
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2559..2577
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2594..2612
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2630..2648
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2666..2684
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2701..2719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2737..2755
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2800..3962
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3031..3953
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3910..3930
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3975..3984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4929..4982
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4989..5585
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4989..5008)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5485..5505
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5586..5642
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5643..6260,6261..6266,6267..6302,6303..6305,6306..6341,
                     6342..6344,6345..6380,6381..6389)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6439..7027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6492..6512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6910..6921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7311..7432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7348..7367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7402..7421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7621..7683
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7707..7725)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7746..7762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7746..7762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7759..7781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7770..7786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7794..7800,7801..7818,7819..7824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7839..7860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7977..7994)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8148..8736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8228..8247)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8907..9698,9699..9767))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9530..9549
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9768..9872)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccttaac
      661 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
      721 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
      781 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
      841 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
      901 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
      961 tagatatcaa gctatcaact ttgtatagaa aagttggtgt cgcacaggat gggttcattc
     1021 tagaattgtt tctccccacc cttcctaact tctctcggat gtccttcaag tgcagctgta
     1081 ggctccgctc ttggcattca ccgcgtgcct taattgtatg acattaaatc aaggtccgct
     1141 gtgaacacgg agagcagagt ctctgatctt gatctagaag caagtgactc attgcaacta
     1201 ttactataat tttctccctg cccttcaccc tctcttggag ctctaggctt ggagtcccta
     1261 ctcctagagt tgtagctctg agcagtactc ccacctacta gaaggagagc cgaggtcgga
     1321 tacctggtcc cggcgtggcc gctccgtcga gctggaatcc ctgggtggcg tgggcttctc
     1381 tgggctcctc tggctcctgt ggccagggcg gccggccggg ccatcgcacg cagaagttgt
     1441 gagagtcatt tctactttcg gttatctagc tttatgacgg ctctgtggca ctcatacagc
     1501 tagataacca aagagagaaa cgggcctccc attcgccggc accgctgccg cgctgtgggc
     1561 cccacgtgcc gccgccgacc cggcgcgggg gcctgagcgg actccgctcc cgcagccccg
     1621 ggcgcacgcg gagagccaga ggggaccgac agccactttg tacaagaaag ctgggtctta
     1681 cttgtggccc agataggcac ccagggtgat gcaagctccc accagggcca aactgagcag
     1741 agtcttcaga gacagccagg agaaatcaaa cagaggccgc atgctggggc cgtacagttc
     1801 cacaaaggca tcccagcctc cgttatcctg gatccaggtg tgcaggtgcc ggttcaggta
     1861 ctcagtcatc cacagggcga tgttgtccac caggggcgac atctcccggt tgacgctctc
     1921 cacacacatg accccaccga actcaaagaa ggccacaatc ctcccccagt tcaccccgtc
     1981 cctgaagagc tcctccacca ccgtggcaaa gcgtccccgc gcggtgaagg gcgtcaggtg
     2041 cagctggctg gacatctcgg cgaagtcgcg gcggtagcgg cgggagaagt cgtcgccggc
     2101 ctggcggagg gtcaggtgga ccacaggtgg caccgggctg agcgcaggcc ccgcggcggc
     2161 gccgggggca gccggggtct gcagcggcga ggtcctggcg accgggtccc gggatgcggc
     2221 tggatggggc gtgtgcccgg gctgggagga gaagatgccc ggtgcggggg cggcccccgg
     2281 gggcgcggcg cccacatctc ccgcatccca ctcgtagccc ctctgcgaca gcttataatg
     2341 gatgtacttc atcactatct cccggttatc gtaccccgtt ctcccagcgt gcgccatggt
     2401 tctatctcct tcgaagcctg cttttttgta caaacttgtg atgctagcgg cgatctgacg
     2461 gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac ctcgacatac
     2521 gttctctatc actgataggg agtaaactcg acatacgttc tctatcactg atagggataa
     2581 actcgacata cgttctctat cactgatagg gagtaaactc gacatacgtt ctctatcact
     2641 gatagggagt aaactcgaca tacgttctct atcactgata gggagtaaac tcgacatcgt
     2701 tctctatcac tgatagggag taaactcgac atacgttctc tatcactgat agggagtaaa
     2761 ctcgattgaa gacgaaaggg ccgaattcaa gcttcgtgag gctccggtgc ccgtcagtgg
     2821 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     2881 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     2941 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3001 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3061 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctccagtac
     3121 gtgattcttg atcccgagct ggagccaggg gcgggccttg cgctttagga gccccttcgc
     3181 ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc
     3241 accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac
     3301 ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccag gatctgcaca
     3361 ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat
     3421 gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag
     3481 ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg
     3541 caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg
     3601 ctccaggggg ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca
     3661 cacaaaggaa aggggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc
     3721 gggcgccgtc caggcacctc gattagttct ggagcttttg gagtacgtcg tctttaggtt
     3781 ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta
     3841 ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt
     3901 ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt
     3961 gaacacgcta ccgggccacc atggccgaag ggagcgtggc aagacagccc gatctgctga
     4021 catgcgacga cgaaccaatc cacattccag gagctatcca gcctcatgga ctgctgctgg
     4081 ctctggccgc tgacatgact atcgtggcag gctctgataa cctgccagag ctgaccgggc
     4141 tggctatcgg agcactgatt ggccggtccg cagccgacgt gttcgattct gagactcaca
     4201 atagactgac catcgccctg gctgaacctg gggctgcagt gggagcccca attaccgtcg
     4261 gcttcacaat gcgaaaggac gctggcttta tcgggtcctg gcaccgccat gatcagctga
     4321 tttttctgga gctggaacca cctcagagag acgtggcaga gccacaggct ttctttcgga
     4381 gaacaaacag cgccatcagg cgcctgcagg ccgctgagac tctggaatcc gcttgcgcag
     4441 ccgctgcaca ggaagtgagg aagatcaccg ggttcgatcg cgtcatgatc tacagattcg
     4501 cctcagactt tagcggagaa gtgatcgcag aagatcgctg tgccgaggtc gaaagtaaac
     4561 tgggcctgca ttacccagcc tcaaccgtgc ctgcacaggc acgacggctg tatacaatca
     4621 accccgtgcg gatcattccc gacattaatt acagaccagt gcccgtcaca cctgacctga
     4681 acccagtgac tgggaggccc atcgatctga gcttcgccat tctgcgcagc gtgtcccctg
     4741 tccacctgga gtttatgagg aatatcggaa tgcatggcac catgtctatc agtattctga
     4801 gaggcgaaag gctgtggggg ctgatcgtgt gccaccatcg gacaccttac tatgtggacc
     4861 tggacggcag gcaggcttgt gaactggtgg ctcaggtgct ggcttggcag attggcgtga
     4921 tggaagaaga gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc
     4981 ctatgtctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5041 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5101 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5161 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5221 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5281 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5341 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5401 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     5461 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5521 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5581 gtgccgccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc
     5641 ctatgtctag actggacaag agcaaagtca taaacggcgc tctggaatta ctcaatggag
     5701 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     5761 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     5821 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     5881 ggaacaacgc caagtcattc cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     5941 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     6001 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     6061 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     6121 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     6181 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     6241 agctaaagtg cgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc
     6301 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     6361 attttgacct tgacatgctc cccgggtaac taagtaagga tcctctagag ttaacatcga
     6421 gggatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6841 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7021 tccccgcatc gataccgtcg agacctggaa aaacatggag caatcacaag tagcaacaca
     7081 gcagctacca atgctgcttg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7141 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatctagat
     7201 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     7261 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     7321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgctcga
     7441 gtgcatggat cctttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     7501 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     7561 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     7621 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     7681 gggttaaaga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
     7741 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     7801 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     7861 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7921 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     7981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8041 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8101 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8161 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8221 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8281 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8341 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     8401 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     8461 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     8521 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     8581 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     8641 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     8701 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     8761 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     8821 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     8881 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     8941 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9001 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9061 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9121 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9181 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9241 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9301 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9361 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     9421 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     9481 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     9541 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     9601 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     9661 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     9721 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     9781 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     9841 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
