LOCUS       sequence-299848-        7673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of OsTIR1 and mAID-GFP protein in DT40 cells under the 
            control of CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Nishimura K, Yamada R, Hagihara S, Iwasaki R, Uchida N, Kamura T, 
            Takahashi K, Torii KU, Fukagawa T
  TITLE     A super-sensitive auxin-inducible degron system with an engineered 
            auxin-TIR1 pair.
  JOURNAL   Nucleic Acids Res. 2020 Oct 9;48(18):e108. doi: 10.1093/nar/gkaa748.
  PUBMED    32941625
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2020-10-9"; volume: "48(18):e108. doi"; pages: "
            10.1093/nar/gkaa748"
COMMENT     Sequence Label: pAID1.2-CMV-CGFP-mAID
FEATURES             Location/Qualifiers
     source          1..7673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             242..1966
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             1967..2020
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(2447..2466)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     misc_feature    join(2530..3104,3105..3107,3108..3116)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2697..2714)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2924..2943
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3159..3362
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(3363..3365,3366..3368,3369..4082)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3363..3365,3366..3368,3369..4079)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3408..3429)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3669..3688)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4016..4037
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4097..4218
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4134..4153)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4188..4207
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4225..4680)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4362..4383)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4574..4593
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4707..4811
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4813..5170
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4813..5004
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4836..4856)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5021..5156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5205..5999
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5205..5999
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5259..5278)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5869..5888
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6187..6206)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6231..6278
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6607..7195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7096..7115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7370..7673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctgccac
      241 catgacatac tttcctgaag aggtcgtcga acacattttt agcttcctgc ctgcacagag
      301 agatagaaac acagtgagcc tggtctgcaa agtgtggtac gagatcgaac gcctgagccg
      361 gagaggagtg ttcgtcggca actgctatgc tgtgagagca ggcagggtcg ccgctaggtt
      421 tccaaatgtg cgcgcactga ccgtcaaggg gaaaccccac ttcgccgact ttaacctggt
      481 gccccctgat tggggaggat acgccggccc ttggatcgag gcagccgctc gcggctgtca
      541 tggactggag gaactgcgca tgaagcgaat ggtggtctct gacgaaagtc tggagctgct
      601 ggctcggagc ttccctaggt ttcgcgcact ggtgctgatt tcttgcgaag gcttcagcac
      661 cgatggactg gcagccgtgg cctcccactg taagctgctg cgggagctgg acctccagga
      721 gaatgaagtg gaggatagag gccccagatg gctgtcttgc ttcccagact catgtaccag
      781 cctggtgtcc ctgaactttg cctgcatcaa aggcgaagtg aatgctgggt ccctggagcg
      841 gctggtctca agaagcccca acctgaggtc tctgcggctg aaccggagcg tgagcgtgga
      901 cactctggct aagattctgc tgagaacccc taacctggag gatctgggaa ccggcaatct
      961 gacagacgat ttccagacag aatcctactt taaactgact tctgccctgg agaagtgtaa
     1021 aatgctgagg agtctgtcag gattctggga tgcttcaccc gtgtgcctga gctttatcta
     1081 ccctctgtgt gcacagctga caggcctgaa cctgagctat gcaccaaccc tggacgccag
     1141 tgatctgaca aagatgatct cacgctgcgt gaaactccag cgactgtggg tgctggactg
     1201 tatttccgat aaggggctcc aggtggtcgc cagctcctgc aaggacctcc aggagctgag
     1261 agtgttccca tctgattttt acgtggccgg atatagtgct gtcactgagg aaggcctggt
     1321 ggcagtctca ctgggatgcc caaagctgaa cagcctgctg tatttctgtc atcagatgac
     1381 taatgctgca ctggtgaccg tcgccaagaa ctgccctaat ttcacccgat ttcggctgtg
     1441 tattctggaa ccaggcaaac ccgacgtggt cacatcccag ccactggatg aagggtttgg
     1501 agctatcgtg agagagtgca agggactcca gaggctgagc atttccggcc tgctgacaga
     1561 caaagtgttc atgtacatcg gcaagtatgc taagcagctg gagatgctga gcattgcatt
     1621 tgccggagac tccgataagg gcatgatgca cgtgatgaac gggtgtaaga atctgcgaaa
     1681 actggaaatc cgggacagcc ctttcgggga tgccgctctg ctgggaaact ttgccagata
     1741 cgagacaatg aggagcctgt ggatgtctag ttgcaatgtg actctgaagg gctgtcaggt
     1801 cctggctagt aaaatgccta tgctgaacgt ggaagtcatt aatgagcggg acgggtctaa
     1861 cgaaatggag gaaaatcatg gcgacctgcc aaaggtggag aaactgtatg tgtatcggac
     1921 caccgcaggg gcaagagatg atgctcccaa ctttgtgaag attctggagg gccgcggcag
     1981 cctgctgacc tgcggcgacg tggaggagaa ccccggcccc atgaaaacat ttaacatttc
     2041 tcaacaagat ctagaattag tagaagtagc gacagagaag attacaatgc tttatgagga
     2101 taataaacat catgtgggag cggcaattcg tacgaaaaca ggagaaatca tttcggcagt
     2161 acatattgaa gcgtatatag gacgagtaac tgtttgtgca gaagccattg cgattggtag
     2221 tgcagtttcg aatggacaaa aggattttga cacgattgta gctgttagac acccttattc
     2281 tgacgaagta gatagaagta ttcgagtggt aagtccttgt ggtatgtgta gggagttgat
     2341 ttcagactat gcaccagatt gttttgtgtt aatagaaatg aatggcaagt tagtcaaaac
     2401 tacgattgaa gaactcattc cactcaaata tacccgaaat taaggatctt tttccctctg
     2461 ccaaaaatta tggggacatc atgaagcccc ttgaggtgga acgatcattc aagagatccc
     2521 cgggatccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     2581 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2641 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2701 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2761 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2821 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2881 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2941 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3001 gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3061 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgt
     3121 gcgatatcgg agctggtgca ggcgctggag cgggtgccaa ggagaagagt gcttgtccta
     3181 aagatccagc caaacctccg gccaaggcac aagttgtggg atggccaccg gtgagatcat
     3241 accggaagaa cgtgatggtt tcctgccaaa aatcaagcgg tggcccggag gcggcggcgt
     3301 tcgtgaaggt atcaatggac ggagcaccgt acttgaggaa aatcgatttg aggatgtata
     3361 aaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3421 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3481 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3541 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3601 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3661 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     3721 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     3781 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     3841 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     3901 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3961 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4021 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4081 gacaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4141 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4201 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     4261 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4321 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4381 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4441 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4501 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4561 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4621 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4801 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     4861 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4921 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4981 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5041 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5101 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5161 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5221 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5281 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5341 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     5401 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     5461 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     5521 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5581 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5641 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5701 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     5761 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5821 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5881 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5941 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6001 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6061 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6121 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     6181 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     6241 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     6301 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     6361 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     6421 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     6481 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6721 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6961 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7081 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7141 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7201 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7261 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat
     7321 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7381 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7441 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7501 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7561 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7621 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atg
//
