LOCUS       sequence-299885-        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLS-V5-MCP-APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY
  TITLE     RNA-protein interaction mapping via MS2- or Cas13-based APEX 
            targeting.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117.
  PUBMED    32839320
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117."
COMMENT     Sequence Label: MCP-APEX2
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..27
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             34..75
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atggctccaa agaagaagcg taaggtaggc agcggcaagc ccatccccaa ccccctgctg
       61 ggcctggaca gcaccggcag cgctagcaat ttcactcaat ttgtgctcgt ggataacgga
      121 ggaacgggcg acgtgaccgt ggcaccaagc aactttgcta atggagtggc cgagtggatt
      181 agctcaaata gcaggtccca ggcttacaag gtcacctgta gtgtccggca atcatccgcc
      241 cagaatcgga aatatactat taaggtagaa gtgccgaaag tggcaactca aaccgtgggg
      301 ggcgaggaat tgcctgtggc cggctggaga agctacctca acatggaact caccattcca
      361 atcttcgcca caaacagcga ctgtgagctc atcgtcaaag ccatgcaggg tctgctgaag
      421 gacggcaatc ctattccatc tgcaatcgcg gccaattccg gcatttatgg atccaagggc
      481 tcgggctcga cctcgggctc cggagggaaa tcatacccaa cagtgtccgc agactaccag
      541 gatgccgtgg agaaagccaa gaagaagctg agagggttta tcgcagaaaa gaggtgcgca
      601 cctctgatgc tgagactggc tttccacagc gcaggcacct ttgacaaggg aaccaaaaca
      661 ggcggaccct ttggaacaat caagcaccct gctgaactgg cacatagtgc caacaatggg
      721 ctggacatcg ccgtgcggct gctggaacct ctgaaagcag agttcccaat tctgtcctac
      781 gccgattttt atcagctggc aggagtggtc gctgtggagg tcactggggg ccccaaggtg
      841 cctttccacc caggacggga ggacaaacca gaaccacctc cagaggggcg cctgccagat
      901 ccgacaaagg gctccgacca tctgcgagat gtgtttggga aagctatggg cctgactgac
      961 caggatatcg tcgcactgtc tggagggcac accattggcg ccgctcataa ggaaaggtca
     1021 ggcttcgagg gaccctggac aagcaacccc ctgattttcg acaattctta ctttactgaa
     1081 ctgctgagtg gagagaagga agggctgctg cagctgccca gcgacaaagc cctgctgtcc
     1141 gatcccgtgt tcagacctct ggtcgataag tatgcagccg acgaggatgc ttttttcgca
     1201 gattacgcag aagcacatca gaagctgtca gaactgggat ttgccgacgc ctaa
//
