LOCUS       sequence-299886-        3303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BPNLS-dRfxCas13d-dsRBD-BPNLS-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY
  TITLE     RNA-protein interaction mapping via MS2- or Cas13-based APEX 
            targeting.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117.
  PUBMED    32839320
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117."
COMMENT     Sequence Label: dCas13d-dsRBD
FEATURES             Location/Qualifiers
     source          1..3303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..57
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             67..2964
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             3253..3273
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3274..3300
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgaagcgga ctgctgatgg cagtgaattt gagagcccca agaagaagag aaaggtggag
       61 gccagcatcg aaaaaaaaaa gtccttcgcc aagggcatgg gcgtgaagtc cacactcgtg
      121 tccggctcca aagtgtacat gacaaccttc gccgaaggca gcgacgccag gctggaaaag
      181 atcgtggagg gcgacagcat caggagcgtg aatgagggcg aggccttcag cgctgaaatg
      241 gccgataaaa acgccggcta taagatcggc aacgccaaat tcagccatcc taagggctac
      301 gccgtggtgg ctaacaaccc tctgtataca ggacccgtcc agcaggatat gctcggcctg
      361 aaggaaactc tggaaaagag gtacttcggc gagagcgctg atggcaatga caatatttgt
      421 atccaggtga tccataacat cctggacatt gaaaaaatcc tcgccgaata cattaccaac
      481 gccgcctacg ccgtcaacaa tatctccggc ctggataagg acattattgg attcggcaag
      541 ttctccacag tgtataccta cgacgaattc aaagaccccg agcaccatag ggccgctttc
      601 aacaataacg ataagctcat caacgccatc aaggcccagt atgacgagtt cgacaacttc
      661 ctcgataacc ccagactcgg ctatttcggc caggcctttt tcagcaagga gggcagaaat
      721 tacatcatca attacggcaa cgaatgctat gacattctgg ccctcctgag cggactggcg
      781 cactgggtgg tcgctaacaa cgaagaagag tccaggatct ccaggacctg gctctacaac
      841 ctcgataaga acctcgacaa cgaatacatc tccaccctca actacctcta cgacaggatc
      901 accaatgagc tgaccaactc cttctccaag aactccgccg ccaacgtgaa ctatattgcc
      961 gaaactctgg gaatcaaccc tgccgaattc gccgaacaat atttcagatt cagcattatg
     1021 aaagagcaga aaaacctcgg attcaatatc accaagctca gggaagtgat gctggacagg
     1081 aaggatatgt ccgagatcag gaaaaatcat aaggtgttcg actccatcag gaccaaggtc
     1141 tacaccatga tggactttgt gatttatagg tattacatcg aagaggatgc caaggtggct
     1201 gccgccaata agtccctccc cgataatgag aagtccctga gcgagaagga tatctttgtg
     1261 attaacctga ggggctcctt caacgacgac cagaaggatg ccctctacta cgatgaagct
     1321 aatagaattt ggagaaagct cgaaaatatc atgcacaaca tcaaggaatt taggggaaac
     1381 aagacaagag agtataagaa gaaggacgcc cctagactgc ccagaatcct gcccgctggc
     1441 cgtgatgttt ccgccttcag caaactcatg tatgccctga ccatgttcct ggatggcaag
     1501 gagatcaacg acctcctgac caccctgatt aataaattcg ataacatcca gagcttcctg
     1561 aaggtgatgc ctctcatcgg agtcaacgct aagttcgtgg aggaatacgc ctttttcaaa
     1621 gactccgcca agatcgccga tgagctgagg ctgatcaagt ccttcgctag aatgggagaa
     1681 cctattgccg atgccaggag ggccatgtat atcgacgcca tccgtatttt aggaaccaac
     1741 ctgtcctatg atgagctcaa ggccctcgcc gacacctttt ccctggacga gaacggaaac
     1801 aagctcaaga aaggcaagca cggcatgaga aatttcatta ttaataacgt gatcagcaat
     1861 aaaaggttcc actacctgat cagatacggt gatcctgccc acctccatga gatcgccaaa
     1921 aacgaggccg tggtgaagtt cgtgctcggc aggatcgctg acatccagaa aaaacagggc
     1981 cagaacggca agaaccagat cgacaggtac tacgaaactt gtatcggaaa ggataagggc
     2041 aagagcgtga gcgaaaaggt ggacgctctc acaaagatca tcaccggaat gaactacgac
     2101 caattcgaca agaaaaggag cgtcattgag gacaccggca gggaaaacgc cgagagggag
     2161 aagtttaaaa agatcatcag cctgtacctc accgtgatct accacatcct caagaatatt
     2221 gtcaatatca acgccaggta cgtcatcgga ttccattgcg tcgagcgtga tgctcaactg
     2281 tacaaggaga aaggctacga catcaatctc aagaaactgg aagagaaggg attcagctcc
     2341 gtcaccaagc tctgcgctgg cattgatgaa actgcccccg ataagagaaa ggacgtggaa
     2401 aaggagatgg ctgaaagagc caaggagagc attgacagcc tcgagagcgc caaccccaag
     2461 ctgtatgcca attacatcaa atacagcgac gagaagaaag ccgaggagtt caccaggcag
     2521 attaacaggg agaaggccaa aaccgccctg aacgcctacc tgaggaacac caagtggaat
     2581 gtgatcatca gggaggacct cctgagaatt gacaacaaga catgtaccct gttcgcaaac
     2641 aaggccgtcg ccctggaagt ggccaggtat gtccacgcct atatcaacga cattgccgag
     2701 gtcaattcct acttccaact gtaccattac atcatgcaga gaattatcat gaatgagagg
     2761 tacgagaaaa gcagcggaaa ggtgtccgag tacttcgacg ctgtgaatga cgagaagaag
     2821 tacaacgata ggctcctgaa actgctgtgt gtgcctttcg gctactgtat ccccaggttt
     2881 aagaacctga gcatcgaggc cctgttcgat aggaacgagg ccgccaagtt cgacaaggag
     2941 aaaaagaagg tgtccggcaa ttccggatcc ggagcaggcg atttgtccgc aggtttcttc
     3001 atggaagaac tgaacactta tcgccagaaa cagggagttg tgctgaagta ccaagagttg
     3061 ccgaactcag gcccacctca cgaccgaaga ttcacttttc aagtaattat cgatggtaga
     3121 gagttcccag aaggcgaagg ccgaagtaag aaagaagcga agaacgcagc cgctaaattg
     3181 gcagtcgaaa tcctcaataa agaaaagaaa gcgggatcca agcgcaccgc cgatggttcc
     3241 gagttcgaaa gccccaagaa aaagcgcaag gtctacccat acgatgttcc agattacgct
     3301 taa
//
