LOCUS       sequence-299890-        8700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of VSV tagged PTK7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Gartner S, Gunesch A, Knyazeva T, Wolf P, Hogel B, Eiermann W, 
            Ullrich A, Knyazev P, Ataseven B
  TITLE     PTK 7 is a transforming gene and prognostic marker for breast cancer
            and nodal metastasis involvement.
  JOURNAL   PLoS One. 2014 Jan 7;9(1):e84472. doi: 10.1371/journal.pone.0084472.
            eCollection 2014.
  PUBMED    24409301
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-01-7"; volume: "9(1):e84472. doi"; pages: " 
            10.1371/journal.pone.0084472. eCollection 2014"
COMMENT     Sequence Label: pcDNA3-PTK7-VSV
FEATURES             Location/Qualifiers
     source          1..8700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3965..3997
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     primer_bind     complement(4020..4037)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4057..4074)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4063..4287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4333..4761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4420..4439)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4630..4651
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4770..4790)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4775..5104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4955..5090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5017..5036
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5171..5965
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5225..5244)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5835..5854
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6139..6260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6176..6195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6230..6249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6309..6325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6309..6325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6322..6344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6333..6349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6357..6363,6364..6381,6382..6387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6402..6423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6540..6557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6711..7299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6791..6810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7470..8261,8262..8330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8093..8112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8331..8435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8510..8529)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg cgccctcagc
      721 tccttttcct gagcccgccg cgatgggagc tgcgcgggga tccccggcca gaccccgccg
      781 gttgcctctg ctcagcgtcc tgctgctgcc gctgctgggc ggtacccaga cagccattgt
      841 cttcatcaag cagccgtcct cccaggatgc actgcagggg cgccgggcgc tgcttcgctg
      901 tgaggttgag gctccgggcc cggtacatgt gtactggctg ctcgatgggg cccctgtcca
      961 ggacacggag cggcgtttcg cccagggcag cagcctgagc tttgcagctg tggaccggct
     1021 gcaggactct ggcaccttcc agtgtgtggc tcgggatgat gtcactggag aagaagcccg
     1081 cagtgccaac gcctccttca acatcaaatg gattgaggca ggtcctgtgg tcctgaagca
     1141 tccagcctcg gaagctgaga tccagccaca gacccaggtc acacttcgtt gccacattga
     1201 tgggcaccct cggcccacct accaatggtt ccgagatggg accccccttt ctgatggtca
     1261 gagcaaccac acagtcagca gcaaggagcg gaacctgacg ctccggccag ctggtcctga
     1321 gcatagtggg ctgtattcct gctgcgccca cagtgctttt ggccaggctt gcagcagcca
     1381 gaacttcacc ttgagcattg ctgatgaaag ctttgccagg gtggtgctgg caccccagga
     1441 cgtggtagta gcgaggtatg aggaggccat gttccattgc cagttctcag cccagccacc
     1501 cccgagcctg cagtggctct ttgaggatga gactcccatc actaaccgca gtcgcccccc
     1561 acacctccgc agagccacag tgtttgccaa cgggtctctg ctgctgaccc aggtccggcc
     1621 acgcaatgca gggatctacc gctgcattgg ccaggggcag aggggcccac ccatcatcct
     1681 ggaagccaca cttcacctag cagagattga agacatgccg ctatttgagc cacgggtgtt
     1741 tacagctggc agcgaggagc gtgtgacctg ccttcccccc aagggtctgc cagagcccag
     1801 cgtgtggtgg gagcacgcgg gagtccggct gcccacccat ggcagggtct accagaaggg
     1861 ccacgagctg gtgttggcca atattgctga aagtgatgct ggtgtctaca cctgccacgc
     1921 ggccaacctg gctggtcagc ggagacagga tgtcaacatc actgtggcca ctgtgccctc
     1981 ctggctgaag aagccccaag acagccagct ggaggagggc aaacccggct acttggattg
     2041 cctgacccag gccacaccaa aacctacagt tgtctggtac agaaaccaga tgctcatctc
     2101 agaggactca cggttcgagg tcttcaagaa tgggaccttg cgcatcaaca gcgtggaggt
     2161 gtatgatggg acatggtacc gttgtatgag cagcacccca gccggcagca tcgaggcgca
     2221 agcccgtgtc caagtgctgg aaaagctcaa gttcacacca ccaccccagc cacagcagtg
     2281 catggagttt gacaaggagg ccacggtgcc ctgttcagcc acaggccgag agaagcccac
     2341 tattaagtgg gaacgggcag atgggagcag cctcccagag tgggtgacag acaacgctgg
     2401 gaccctgcat tttgcccggg tgactcgaga tgacgctggc aactacactt gcattgcctc
     2461 caacgggccg cagggccaga ttcgtgccca tgtccagctc actgtggcag tttttatcac
     2521 cttcaaagtg gaaccagagc gtacgactgt gtaccagggc cacacagccc tactgcagtg
     2581 cgaggcccag ggggacccca agccgctgat tcagtggaaa ggcaaggacc gcatcctgga
     2641 ccccaccaag ctgggaccca ggatgcacat cttccagaat ggctccctgg tgatccatga
     2701 cgtggcccct gaggactcag gccgctacac ctgcattgca ggcaacagct gcaacatcaa
     2761 gcacacggag gcccccctct atgtcgtgga caagcctgtg ccggaggagt cggagggccc
     2821 tggcagccct cccccctaca agatgatcca gaccattggg ttgtcggtgg gtgccgctgt
     2881 ggcctacatc attgccgtgc tgggcctcat gttctactgc aagaagcgct gcaaagccaa
     2941 gcggctgcag aagcagcccg agggcgagga gccagagatg gaatgcctca acggtgggcc
     3001 tttgcagaac gggcagccct cagcagagat ccaagaagaa gtggccttga ccagcttggg
     3061 ctccggcccc gcggccacca acaaacgcca cagcacaagt gataagatgc acttcccacg
     3121 gtctagcctg cagcccatca ccacgctggg gaagagtgag tttggggagg tgttcctggc
     3181 aaaggctcag ggcttggagg agggagtggc agagaccctg gtacttgtga agagcctgca
     3241 gagcaaggat gagcagcagc agctggactt ccggagggag ttggagatgt ttgggaagct
     3301 gaaccacgcc aacgtggtgc ggctcctggg gctgtgccgg gaggctgagc cccactacat
     3361 ggtgctggaa tatgtggatc tgggagacct caagcagttc ctgaggattt ccaagagcaa
     3421 ggatgaaaaa ttgaagtcac agcccctcag caccaagcag aaggtggccc tatgcaccca
     3481 ggtagccctg ggcatggagc acctgtccaa caaccgcttt gtgcataagg acttggctgc
     3541 gcgtaactgc ctggtcagtg cccagagaca agtgaaggtg tctgccctgg gcctcagcaa
     3601 ggatgtgtac aacagtgagt actaccactt ccgccaggcc tgggtgccgc tgcgctggat
     3661 gtcccccgag gccatcctgg agggtgactt ctctaccaag tctgatgtct gggccttcgg
     3721 tgtgctgatg tgggaagtgt ttacacatgg agagatgccc catggtgggc aggcagatga
     3781 tgaagtactg gcagatttgc aggctgggaa ggctagactt cctcagcccg agggctgccc
     3841 ttccaaactc tatcggctga tgcagcgctg ctgggccctc agccccaagg accggccctc
     3901 cttcagtgag attgccagcg ccctgggaga cagcaccgtg gacagcaagc cgtctagagg
     3961 gccctacacc gacatcgaga tgaaccggct gggcaagtaa ctcgagctag cggccgcggc
     4021 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4081 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4141 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4201 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4261 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     4321 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4381 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4441 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4501 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4561 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4621 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4681 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4741 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4801 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4861 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4921 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4981 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5041 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5101 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     5161 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5221 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5281 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5341 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5401 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5461 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5521 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5581 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5641 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5701 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5761 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5821 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5881 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5941 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6001 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6061 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6121 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6241 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8461 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     8521 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     8581 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     8641 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
