LOCUS       sequence-299898-        3072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Optogenetic tool for blue-light inhibition and red-light excitation 
            of neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Vierock J, Rodriguez-Rozada S, Dieter A, Pieper F, Sims R, Tenedini 
            F, Bergs ACF, Bendifallah I, Zhou F, Zeitzschel N, Ahlbeck J, 
            Augustin S, Sauter K, Papagiakoumou E, Gottschalk A, Soba P, 
            Emiliani V, Engel AK, Hegemann P, Wiegert JS
  TITLE     BiPOLES is an optogenetic tool developed for bidirectional 
            dual-color control of neurons.
  JOURNAL   Nat Commun. 2021 Jul 26;12(1):4527. doi: 10.1038/s41467-021-24759-5.
  PUBMED    34312384
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-26"; volume: "12(1):4527. doi"; pages: " 
            10.1038/s41467-021-24759-5"
COMMENT     Sequence Label: Ef1a-DIO-BiPOLES-mCerulean
FEATURES             Location/Qualifiers
     source          1..3072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1000..1021)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1261..1280)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1608..1629
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2029..3072
                     /codon_start=1
                     /product="red light-drivable channelrhodopsin from 
                     Chlamydomonas noctigama (Klapoetke et al., 2014)"
                     /label=Chrimson
                     /translation="ELISSATRSLFAAGGINPWPNPYHHEDMGCGGMTPTGECFSTEWW
                     CDPSYGLSDAGYGYCFVEATGGYLVVGVEKKQAWLHSRGTPGEKIGAQVCQWIAFSIAI
                     ALLTFYGFSAWKATCGWEEVYVCCVEVLFVTLEIFKEFSSPATVYLSTGNHAYCLRYFE
                     WLLSCPVILIKLSNLSGLKNDYSKRTMGLIVSCVGMIVFGMAAGLATDWLKWLLYIVSC
                     IYGGYMYFQAAKCYVEANHSVPKGHCRMVVKLMAYAYFASWGSYPILWAVGPEGLLKLS
                     PYANSIGHSICDIIAKEFWTFLAHHLRIKIHEHILIHGDIRKTTKMEIGGEEVEVEEFV
                     EEEDEDTV"
ORIGIN
        1 atggcatcac aggtcgtcta tggggaatgg gcatcaactc acaccgagtg ctacaatatg
       61 tccagaatcg actccacctt cgtctcactg ctgcagctgg tgtgggccgt ggtctcaggc
      121 tgccagacaa tcttcatgat tagcagggct cccaaggtcc cttgggagtc cgtgtacctg
      181 ccctttgtcg aatctatcac ttatgcactg gccagtactg gcaacggaac cctgcagatg
      241 agggacggca gattctttcc ttggagccga atggcctcct ggctgtgcac ctgtccaatc
      301 atgctgggac agatttctaa catggctctg gtgaagtaca aaagtatccc actgaatccc
      361 attgcacagg ccgcttccat cattagagtg gtcatgggaa tcaccgctac aatttctccc
      421 gcagagtata tgaagtggct gttctttttc tttggggcca cctgcctggt gttcgagtac
      481 agtgtggtct tcacaatctt tcaagtgggg ctgtatggct ttgaatctgt cgggacacct
      541 ctggcacaga aggtggtggt gaggatcaag atgctgcgcc tgatcttctt tattgcttgg
      601 actatgttcc caatcgtgtg gctgatttca cccaccggag tctgcgtgat ccacgagaac
      661 gtgagcgcca ttctgtacct gctggctgac ggcctgtgca aaaatacata tggagtgatc
      721 ctgtggagca ctgcctgggg agtgctggaa gggaagtggg accccgcatg tctgccaggg
      781 caggagaaac ccgaagccga cgatcctttt ggcctgaatc acgagaagaa tgcccctcct
      841 aacgacgaag tcaatatcag aatgtttgga aggggacgta cggcggccaa gagcaggatc
      901 accagcgagg gcgagtacat ccccctggac cagatcgaca tcaacgtggc ggtacccgtg
      961 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1021 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1081 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1141 accaccctga gctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     1201 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1261 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1321 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1381 gagtacaacg ccatccacgg caacgtctat atcaccgccg acaagcagaa gaacggcatc
     1441 aaggccaact tcggcctcaa ctgcaacatc gaggacggca gcgtgcagct cgccgaccac
     1501 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1561 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1621 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtatac cggtgcagcc
     1681 ctgcaggaga agaagtcatg cagccagcgc atggccgaat tccggcaata ctgttggaac
     1741 ccggacactg ggcagatgct gggccgcacc ccagcccggt gggtgtggat cagcctgtac
     1801 tatgcagctt tctacgtggt catgactggg ctctttgcct tgtgcatcta tgtgctgatg
     1861 cagaccattg atccctacac ccccgactac caggaccagt taaagtcacc gggggtaacc
     1921 ttgagaccgg atgtgtatgg ggaaagaggg ctgcagattt cctacaacat ctctgaaaac
     1981 agctctagac aggcccagat caccggacgt ccggagggag ctgtacaaga gctgatcagc
     2041 agcgccacca gatctctgtt tgccgccgga ggcatcaacc cttggcctaa cccctaccac
     2101 cacgaggaca tgggctgtgg aggaatgaca cctacaggcg agtgcttcag caccgagtgg
     2161 tggtgtgacc cttcttacgg actgagcgac gccggatacg gatattgctt cgtggaggcc
     2221 acaggcggct acctggtcgt gggagtggag aagaagcagg cttggctgca cagcagaggc
     2281 acaccaggag aaaagatcgg cgcccaggtc tgccagtgga ttgctttcag catcgccatc
     2341 gccctgctga cattctacgg cttcagcgcc tggaaggcca cttgcggttg ggaggaggtc
     2401 tacgtctgtt gcgtcgaggt gctgttcgtg accctggaga tcttcaagga gttcagcagc
     2461 cccgccacag tgtacctgtc taccggcaac cacgcctatt gcctgcgcta cttcgagtgg
     2521 ctgctgtctt gccccgtgat cctgatcaag ctgagcaacc tgagcggcct gaagaacgac
     2581 tacagcaagc ggaccatggg cctgatcgtg tcttgcgtgg gaatgatcgt gttcggcatg
     2641 gccgcaggac tggctaccga ttggctcaag tggctgctgt atatcgtgtc ttgcatctac
     2701 ggcggctaca tgtacttcca ggccgccaag tgctacgtgg aagccaacca cagcgtgcct
     2761 aaaggccatt gccgcatggt cgtgaagctg atggcctacg cttacttcgc ctcttggggc
     2821 agctacccaa tcctctgggc agtgggacca gaaggactgc tgaagctgag cccttacgcc
     2881 aacagcatcg gccacagcat ctgcgacatc atcgccaagg agttttggac cttcctggcc
     2941 caccacctga ggatcaagat ccacgagcac atcctgatcc acggcgacat ccggaagacc
     3001 accaagatgg agatcggagg cgaggaggtg gaagtggaag agttcgtgga ggaggaggac
     3061 gaggacacag tg
//
