LOCUS       sequence-299925-         462 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease 
            cleavable 6His tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 462)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET22-Nsp10-TEV-6His
FEATURES             Location/Qualifiers
     source          1..462
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             421..441
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             442..459
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggctggta atgcaactga ggttcccgcg aatagtacgg ttctgagctt ttgcgccttt
       61 gctgttgacg cagctaaggc atataaggac tacttggcct ccgggggcca gccaatcaca
      121 aactgcgtca agatgttgtg tacgcacacg ggcacaggtc aggcaatcac tgtcacacct
      181 gaagctaaca tggatcagga atcttttggt ggtgccagtt gttgtctgta ctgtcgatgt
      241 catatagatc atcccaaccc gaaagggttt tgtgatctga aaggaaaata cgtccaaatc
      301 ccgaccacgt gcgcgaacga cccggttggg tttacgttga aaaataccgt atgtactgtc
      361 tgtggtatgt ggaagggcta tggctgttca tgcgatcaat tgcgagagcc gatgttgcag
      421 gaaaatctat acttccaagg acatcaccat catcatcact aa
//
