LOCUS       sequence-299935-        1950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease 
            cleavable 6His-SUMO tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1950)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-6His-SUMO-Nsp14
FEATURES             Location/Qualifiers
     source          1..1950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             70..363
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagca tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt
      241 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc
      301 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt
      361 ggatccgctg aaaatgtaac gggcttgttt aaagactgta gtaaagtgat cacaggactc
      421 caccccacac aagcacctac ccacctttcc gtagatacga agttcaaaac ggaaggattg
      481 tgtgtggata taccagggat accaaaggat atgacgtacc gaaggctgat ttccatgatg
      541 ggttttaaga tgaattacca agttaatggc taccccaaca tgttcatcac cagggaggag
      601 gcaattagac acgtaagagc ctggataggc ttcgatgttg aaggttgcca tgcaacccgg
      661 gaagccgtag gcacaaacct tccgttgcag cttggctttt ccacgggcgt caacctcgtt
      721 gccgtaccga ctggctatgt tgacacgccg aacaacaccg atttctctcg cgtaagtgct
      781 aagcctcctc cgggagatca attcaagcat cttatacctc tcatgtacaa gggactgccg
      841 tggaatgtag ttcgcattaa gatcgtgcag atgctctccg atactttgaa aaatctgagc
      901 gacagggtgg tcttcgttct gtgggcacat ggcttcgaac ttacttctat gaaatacttt
      961 gtaaaaatcg ggcccgagcg gacatgttgt ttgtgcgacc gacgagctac ttgttttagc
     1021 acagcatctg acacctacgc atgctggcac cacagtatag gcttcgatta cgtctacaat
     1081 ccctttatga tagacgtaca acaatggggc tttacgggta acttgcagag taatcacgat
     1141 ctttactgcc aagttcatgg gaacgcacac gtggcctcct gcgatgcgat aatgacgagg
     1201 tgcttggccg tgcacgagtg ctttgttaag cgggtcgatt ggactataga gtatcccata
     1261 atcggtgacg aacttaaaat taatgctgca tgcaggaaag tgcagcacat ggtagtaaaa
     1321 gcagcgctcc tcgcggataa gtttcctgtg ctccacgata tcggcaaccc taaggccatt
     1381 aaatgcgtcc cccaagctga tgtagagtgg aaattctatg acgctcagcc atgtagtgac
     1441 aaagcataca agattgaaga attgttttat tcatatgcga cacactccga taagttcact
     1501 gacggagttt gtctcttttg gaactgtaat gttgataggt atccggcgaa tagcatagtg
     1561 tgtcgatttg acacgcgcgt tctctctaat ttgaacctcc ctggctgcga cggggggtct
     1621 ttgtacgtaa acaagcatgc atttcatacc ccagcttttg acaaatccgc tttcgtaaat
     1681 ctgaaacaac ttcccttctt ctattattca gatagccctt gcgaatctca cggtaagcaa
     1741 gtggttagtg acattgacta tgtaccactt aaaagtgcaa cgtgcattac acgatgcaat
     1801 ttggggggcg cggtttgccg acaccatgcc aacgaataca gattgtacct ggatgcatat
     1861 aacatgatga taagtgcagg gtttagtctt tgggtgtata agcagttcga tacgtataat
     1921 ctctggaaca catttacccg attgcagtaa
//
