LOCUS       sequence-299939-        1635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease 
            cleavable Strep tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1635)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-Strep-TEV-Nsp14
FEATURES             Location/Qualifiers
     source          1..1635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggatggt cacaccccca atttgaaaag gaaaatctat acttccaagg agctgaaaat
       61 gtaacgggct tgtttaaaga ctgtagtaaa gtgatcacag gactccaccc cacacaagca
      121 cctacccacc tttccgtaga tacgaagttc aaaacggaag gattgtgtgt ggatatacca
      181 gggataccaa aggatatgac gtaccgaagg ctgatttcca tgatgggttt taagatgaat
      241 taccaagtta atggctaccc caacatgttc atcaccaggg aggaggcaat tagacacgta
      301 agagcctgga taggcttcga tgttgaaggt tgccatgcaa cccgggaagc cgtaggcaca
      361 aaccttccgt tgcagcttgg cttttccacg ggcgtcaacc tcgttgccgt accgactggc
      421 tatgttgaca cgccgaacaa caccgatttc tctcgcgtaa gtgctaagcc tcctccggga
      481 gatcaattca agcatcttat acctctcatg tacaagggac tgccgtggaa tgtagttcgc
      541 attaagatcg tgcagatgct ctccgatact ttgaaaaatc tgagcgacag ggtggtcttc
      601 gttctgtggg cacatggctt cgaacttact tctatgaaat actttgtaaa aatcgggccc
      661 gagcggacat gttgtttgtg cgaccgacga gctacttgtt ttagcacagc atctgacacc
      721 tacgcatgct ggcaccacag tataggcttc gattacgtct acaatccctt tatgatagac
      781 gtacaacaat ggggctttac gggtaacttg cagagtaatc acgatcttta ctgccaagtt
      841 catgggaacg cacacgtggc ctcctgcgat gcgataatga cgaggtgctt ggccgtgcac
      901 gagtgctttg ttaagcgggt cgattggact atagagtatc ccataatcgg tgacgaactt
      961 aaaattaatg ctgcatgcag gaaagtgcag cacatggtag taaaagcagc gctcctcgcg
     1021 gataagtttc ctgtgctcca cgatatcggc aaccctaagg ccattaaatg cgtcccccaa
     1081 gctgatgtag agtggaaatt ctatgacgct cagccatgta gtgacaaagc atacaagatt
     1141 gaagaattgt tttattcata tgcgacacac tccgataagt tcactgacgg agtttgtctc
     1201 ttttggaact gtaatgttga taggtatccg gcgaatagca tagtgtgtcg atttgacacg
     1261 cgcgttctct ctaatttgaa cctccctggc tgcgacgggg ggtctttgta cgtaaacaag
     1321 catgcatttc ataccccagc ttttgacaaa tccgctttcg taaatctgaa acaacttccc
     1381 ttcttctatt attcagatag cccttgcgaa tctcacggta agcaagtggt tagtgacatt
     1441 gactatgtac cacttaaaag tgcaacgtgc attacacgat gcaatttggg gggcgcggtt
     1501 tgccgacacc atgccaacga atacagattg tacctggatg catataacat gatgataagt
     1561 gcagggttta gtctttgggt gtataagcag ttcgatacgt ataatctctg gaacacattt
     1621 acccgattgc agtag
//
