LOCUS       sequence-299942-        1629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease 
            cleavable 6His tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1629)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-6His-TEV-Nsp14
FEATURES             Location/Qualifiers
     source          1..1629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             25..45
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggacatc accatcatca tcacgaaaat ctatacttcc aaggagctga aaatgtaacg
       61 ggcttgttta aagactgtag taaagtgatc acaggactcc accccacaca agcacctacc
      121 cacctttccg tagatacgaa gttcaaaacg gaaggattgt gtgtggatat accagggata
      181 ccaaaggata tgacgtaccg aaggctgatt tccatgatgg gttttaagat gaattaccaa
      241 gttaatggct accccaacat gttcatcacc agggaggagg caattagaca cgtaagagcc
      301 tggataggct tcgatgttga aggttgccat gcaacccggg aagccgtagg cacaaacctt
      361 ccgttgcagc ttggcttttc cacgggcgtc aacctcgttg ccgtaccgac tggctatgtt
      421 gacacgccga acaacaccga tttctctcgc gtaagtgcta agcctcctcc gggagatcaa
      481 ttcaagcatc ttatacctct catgtacaag ggactgccgt ggaatgtagt tcgcattaag
      541 atcgtgcaga tgctctccga tactttgaaa aatctgagcg acagggtggt cttcgttctg
      601 tgggcacatg gcttcgaact tacttctatg aaatactttg taaaaatcgg gcccgagcgg
      661 acatgttgtt tgtgcgaccg acgagctact tgttttagca cagcatctga cacctacgca
      721 tgctggcacc acagtatagg cttcgattac gtctacaatc cctttatgat agacgtacaa
      781 caatggggct ttacgggtaa cttgcagagt aatcacgatc tttactgcca agttcatggg
      841 aacgcacacg tggcctcctg cgatgcgata atgacgaggt gcttggccgt gcacgagtgc
      901 tttgttaagc gggtcgattg gactatagag tatcccataa tcggtgacga acttaaaatt
      961 aatgctgcat gcaggaaagt gcagcacatg gtagtaaaag cagcgctcct cgcggataag
     1021 tttcctgtgc tccacgatat cggcaaccct aaggccatta aatgcgtccc ccaagctgat
     1081 gtagagtgga aattctatga cgctcagcca tgtagtgaca aagcatacaa gattgaagaa
     1141 ttgttttatt catatgcgac acactccgat aagttcactg acggagtttg tctcttttgg
     1201 aactgtaatg ttgataggta tccggcgaat agcatagtgt gtcgatttga cacgcgcgtt
     1261 ctctctaatt tgaacctccc tggctgcgac ggggggtctt tgtacgtaaa caagcatgca
     1321 tttcataccc cagcttttga caaatccgct ttcgtaaatc tgaaacaact tcccttcttc
     1381 tattattcag atagcccttg cgaatctcac ggtaagcaag tggttagtga cattgactat
     1441 gtaccactta aaagtgcaac gtgcattaca cgatgcaatt tggggggcgc ggtttgccga
     1501 caccatgcca acgaatacag attgtacctg gatgcatata acatgatgat aagtgcaggg
     1561 tttagtcttt gggtgtataa gcagttcgat acgtataatc tctggaacac atttacccga
     1621 ttgcagtag
//
