LOCUS       sequence-299945-        1632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease 
            cleavable Strep tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1632)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET28-Nsp14-TEV-Strep
FEATURES             Location/Qualifiers
     source          1..1632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1585..1605
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1606..1629
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggctgaaa atgtaacggg cttgtttaaa gactgtagta aagtgatcac aggactccac
       61 cccacacaag cacctaccca cctttccgta gatacgaagt tcaaaacgga aggattgtgt
      121 gtggatatac cagggatacc aaaggatatg acgtaccgaa ggctgatttc catgatgggt
      181 tttaagatga attaccaagt taatggctac cccaacatgt tcatcaccag ggaggaggca
      241 attagacacg taagagcctg gataggcttc gatgttgaag gttgccatgc aacccgggaa
      301 gccgtaggca caaaccttcc gttgcagctt ggcttttcca cgggcgtcaa cctcgttgcc
      361 gtaccgactg gctatgttga cacgccgaac aacaccgatt tctctcgcgt aagtgctaag
      421 cctcctccgg gagatcaatt caagcatctt atacctctca tgtacaaggg actgccgtgg
      481 aatgtagttc gcattaagat cgtgcagatg ctctccgata ctttgaaaaa tctgagcgac
      541 agggtggtct tcgttctgtg ggcacatggc ttcgaactta cttctatgaa atactttgta
      601 aaaatcgggc ccgagcggac atgttgtttg tgcgaccgac gagctacttg ttttagcaca
      661 gcatctgaca cctacgcatg ctggcaccac agtataggct tcgattacgt ctacaatccc
      721 tttatgatag acgtacaaca atggggcttt acgggtaact tgcagagtaa tcacgatctt
      781 tactgccaag ttcatgggaa cgcacacgtg gcctcctgcg atgcgataat gacgaggtgc
      841 ttggccgtgc acgagtgctt tgttaagcgg gtcgattgga ctatagagta tcccataatc
      901 ggtgacgaac ttaaaattaa tgctgcatgc aggaaagtgc agcacatggt agtaaaagca
      961 gcgctcctcg cggataagtt tcctgtgctc cacgatatcg gcaaccctaa ggccattaaa
     1021 tgcgtccccc aagctgatgt agagtggaaa ttctatgacg ctcagccatg tagtgacaaa
     1081 gcatacaaga ttgaagaatt gttttattca tatgcgacac actccgataa gttcactgac
     1141 ggagtttgtc tcttttggaa ctgtaatgtt gataggtatc cggcgaatag catagtgtgt
     1201 cgatttgaca cgcgcgttct ctctaatttg aacctccctg gctgcgacgg ggggtctttg
     1261 tacgtaaaca agcatgcatt tcatacccca gcttttgaca aatccgcttt cgtaaatctg
     1321 aaacaacttc ccttcttcta ttattcagat agcccttgcg aatctcacgg taagcaagtg
     1381 gttagtgaca ttgactatgt accacttaaa agtgcaacgt gcattacacg atgcaatttg
     1441 gggggcgcgg tttgccgaca ccatgccaac gaatacagat tgtacctgga tgcatataac
     1501 atgatgataa gtgcagggtt tagtctttgg gtgtataagc agttcgatac gtataatctc
     1561 tggaacacat ttacccgatt gcaggaaaat ctatacttcc aaggatggtc acacccccaa
     1621 tttgaaaagt aa
//
