LOCUS       sequence-299965-       13204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Flp-mediated cassette exchange. Expresses tdTomato in the 
            presence of (r)tTA, switching to connective tissue growth factor in 
            the presence of Cre and (r)tTA activity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13204)
  AUTHORS   Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H,
            Joyner AL, Rosello-Diez A
  TITLE     A collection of genetic mouse lines and related tools for inducible 
            and reversible intersectional mis-expression.
  JOURNAL   Development. 2020 May 28;147(10). pii: dev.186650. doi: 
            10.1242/dev.186650.
  PUBMED    32366677
REFERENCE   2  (bases 1 to 13204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-05-28"; volume: "147(10). pii: 
            dev.186650. doi"; pages: " 10.1242/dev.186650"
COMMENT     Sequence Label: pDragon-Ctgf
FEATURES             Location/Qualifiers
     source          1..13204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    414..447
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     promoter        2993..3307
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    3001..3019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3037..3055
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3072..3090
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3108..3126
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3144..3162
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3179..3197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3215..3233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3280..3304
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(3317..3350)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3360..3369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3499..3517)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3767..3786
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3961..3984
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    4797..4845
                     /note="synthetic polyadenylation signal"
     polyA_signal    4854..5330
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5053..5072)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    complement(5334..5367)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6446..7034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6499..6519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6917..6928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7059..7076)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7065..7272
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    9776..9809
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        9836..10335
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9871..9894)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10268..10287
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(10983..10999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10983..10999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10996..11018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11007..11023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11031..11037,11038..11055,11056..11061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11076..11097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11214..11231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11385..11973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11465..11484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12144..12935,12936..13004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12767..12786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13005..13109)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13177..13195
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt accgaagttc
      421 ctattcttca aatagtatag gaacttcttt cggccggcca atcgattgga tccgttttat
      481 gccgcggtta ccgtgaagat atcttaatta agaattcgag ctcgctagag ccccatcctc
      541 actgactccg tcctggagtt ggatgagaga taatggcctt acgttgtgcc aggggagggt
      601 cgggctggat ttagcaagat ttaccttctc caaagagcgg tgctgcagtg gcacagctgc
      661 ccacggaggt gggggggtca ccgtccctgg aggtgatgaa gaactgtggg gatgtggcac
      721 tgagggacat ggccagtggg cacggtgggt gggttggggt tggtcttggg gatcttggag
      781 ggcttttcca gccttcatga tttgacgatt gtatgaacat ctacatggca attctccagc
      841 tgcctgtccc agtcctactg acccagctgt atctctccag gcaagctctt ccaccccttc
      901 tgcttgcatc cagacaccat caaacatgca ggctcagaca cagggaccag cagtgtctgt
      961 ggcctttttg tgctcctctc catgctgggt tttaacttgc tctttgtcct tctatcctat
     1021 cttcttatcc ttaaggctgt tctgaacgct gtgacttgga gagtgtccca gagccctcaa
     1081 cacctgcatg tcccacgtcc atgctgtcct gcacttcctt atccccaaga tctgcctctc
     1141 cgtgatgcac tgaattggca aacatgtgtc accccagacc aacaatgtca cagcaaactc
     1201 ccccttgata ggacaagggg gaatggcttt acactgagac aggggaggtt tgggttggat
     1261 atgaggaggc agtttttccc ccagagggtg gtgacgcact gaacaggttg cccaaggagg
     1321 ctgtggatgc cccatccctg caggcattca aggccaggct ggatgtggct ctgggcagcc
     1381 tgggctgctg gttgatgacc ctgcacatag cagggggttg gatctggatg agcactgtgc
     1441 tcctttgcaa cccaggccgt tctatgattc tgtcattcta aatctctctt tcagcctaaa
     1501 gctttttccc cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag
     1561 aggaaagcga tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc
     1621 ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct
     1681 agcgggggag ggacgtaatt acatccctgg gggctttggg ggggggctgt cccgagctcg
     1741 gtaccgcccc atcctcactg actccgtcct ggagttggat gagagataat ggccttacgt
     1801 tgtgccaggg gagggtcggg ctggatttag caagatttac cttctccaaa gagcggtgct
     1861 gcagtggcac agctgcccac ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac
     1921 tgtggggatg tggcactgag ggacatggcc agtgggcacg gtgggtgggt tggggttggt
     1981 cttggggatc ttggagggct tttccagcct tcatgatttg acgattgtat gaacatctac
     2041 atggcaattc tccagctgcc tgtcccagtc ctactgaccc agctgtatct ctccaggcaa
     2101 gctcttccac cccttctgct tgcatccaga caccatcaaa catgcaggct cagacacagg
     2161 gaccagcagt gtctgtggcc tttttgtgct cctctccatg ctgggtttta acttgctctt
     2221 tgtccttcta tcctatcttc ttatccttaa ggctgttctg aacgctgtga cttggagagt
     2281 gtcccagagc cctcaacacc tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc
     2341 ccaagatctg cctctccgtg atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca
     2401 atgtcacagc aaactccccc ttgataggac aagggggaat ggctttacac tgagacaggg
     2461 gaggtttggg ttggatatga ggaggcagtt tttcccccag agggtggtga cgcactgaac
     2521 aggttgccca aggaggctgt ggatgcccca tccctgcagg cattcaaggc caggctggat
     2581 gtggctctgg gcagcctggg ctgctggttg atgaccctgc acatagcagg gggttggatc
     2641 tggatgagca ctgtgctcct ttgcaaccca ggccgttcta tgattctgtc attctaaatc
     2701 tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg ctcaaagagc
     2761 agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc
     2821 cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg
     2881 ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg
     2941 ggctgtcccg gtacccgggg atccaccggt atcgattacc tatcacaaat tcgagtttac
     3001 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat
     3061 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     3121 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc
     3181 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
     3241 tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca
     3301 gatcgcctat ttaaatataa cttcgtatag catacattat acgaagttat tcgcgagccg
     3361 ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
     3421 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3481 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
     3541 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
     3601 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     3661 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
     3721 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
     3781 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
     3841 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
     3901 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
     3961 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
     4021 agggccgcca ccacctgttc ctggggcatg gtaccggcag caccggcagc ggcagctccg
     4081 gcaccgcctc ctccgaggac aacaacatgg ccgtgattaa ggagtttatg aggtttaaag
     4141 tcaggatgga gggcagcatg aacgggcacg agtttgagat tgaaggagag ggggaaggga
     4201 ggccctatga gggcacccag acagcaaagc tgaaagtgac aaagggcggc cctctgccat
     4261 tcgcatggga catcctgtct cctcagttta tgtatggaag taaagcctac gtgaaacacc
     4321 ctgctgacat tccagattac aagaaactga gcttcccaga ggggtttaag tgggaaagag
     4381 tgatgaactt cgaagacggg ggcctggtga ctgtcaccca ggacagctct ctgcaggacg
     4441 gcactctgat ctataaggtg aaaatgcgcg gcaccaattt tccccctgat ggcccagtca
     4501 tgcagaagaa gacaatgggc tgggaggctt caacagaaag gctgtacccc agagacggcg
     4561 tgctgaaggg agagatccac caggcactga agctgaaaga tggagggcat tatctggtcg
     4621 aattcaaaac catctacatg gccaagaaac ccgtgcagct gcctggctac tattacgtcg
     4681 acacaaagct ggatattact agccataacg aagactacac cattgtggag cagtatgagc
     4741 ggagcgaggg gaggcaccat ctgtttctgt acggcatgga cgagctgtac aagtaaaata
     4801 aaatatcttt attttcattc catctgtgtg ttggtttttt gtgtgagatc tacgggtggc
     4861 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     4921 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     4981 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     5041 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     5101 caatctccgc ctcctgggtt caagcgattc tcctgcctca ggctcccgag ttgttgggat
     5161 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     5221 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     5281 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgataactt
     5341 cgtatagcat acattatacg aagttatcac gcgtgccacc atgctcgcct ccgtcgcagg
     5401 tcccatcagc ctcgccttgg tgctcctcgc tctctgcacc cggcctgcta tgggccagga
     5461 ctgcagcgcg caatgtcagt gcgcagccga agcagcgccg cactgccccg ccggcgtgag
     5521 cctggtgctg gacggctgcg gctgctgccg cgtctgcgcc aagcagctgg gagaactgtg
     5581 tacggagcgt gacccctgcg acccacacaa gggcctcttc tgcgatttcg gctcccccgc
     5641 caaccgcaag atcggagtgt gcactgccaa agatggtgca ccctgtgtct tcggtgggtc
     5701 ggtgtaccgc agcggtgagt ccttccaaag cagctgcaaa taccaatgca cttgcctgga
     5761 tggggccgtg ggctgcgtgc ccctgtgcag catggacgtg cgcctgccca gccctgactg
     5821 ccccttcccg agaagggtca agctgcctgg gaaatgctgc gaggagtggg tgtgtgacga
     5881 gcccaaggac cgcacagcag ttggccctgc cctagctgcc taccgactgg aagacacatt
     5941 tggcccagac ccaactatga tgcgagccaa ctgcctggtc cagaccacag agtggagcgc
     6001 ctgttctaag acctgtggga tgggcatctc cacccgagtt accaatgaca ataccttctg
     6061 cagactggag aagcagagcc gcctctgcat ggtcaggccc tgcgaagctg acctggagga
     6121 aaacattaag aagggcaaaa agtgcatccg gacacctaaa atcgccaagc ctgtcaagtt
     6181 tgagctttct ggctgcacca gtgtgaagac atacagggct aagttctgcg gggtgtgcac
     6241 agacggccgc tgctgcacac cgcacagaac caccactctg ccagtggagt tcaaatgccc
     6301 cgatggcgag atcatgaaaa agaatatgat gttcatcaag acctgtgcct gccattacaa
     6361 ctgtcctggg gacaatgaca tctttgagtc cctgtactac aggaagatgt acggagacat
     6421 ggcgtaatac gtaaagcttg tcgataatca acctctggat tacaaaattt gtgaaagatt
     6481 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6541 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6601 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6661 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6721 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6781 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6841 atcatcgtcc tttccttggc tgctcgccta tgttgccacc tggattctgc gcgggacgtc
     6901 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6961 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7021 ggccgcctcc ccgcatcgac accgagcgct gctcgaggcc tcgactgtgc cttctagttg
     7081 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     7141 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     7201 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7261 gcatgctggg gacacgtggg atcctctagt ctagagcccc atcctcactg actccgtcct
     7321 ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg ctggatttag
     7381 caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac ggaggtgggg
     7441 gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag ggacatggcc
     7501 agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct tttccagcct
     7561 tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc tgtcccagtc
     7621 ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct tgcatccaga
     7681 caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc tttttgtgct
     7741 cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc ttatccttaa
     7801 ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc tgcatgtccc
     7861 acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg atgcactgaa
     7921 ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc ttgataggac
     7981 aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga ggaggcagtt
     8041 tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt ggatgcccca
     8101 tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg
     8161 atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca
     8221 ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
     8281 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     8341 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     8401 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     8461 gtaattacat ccctgggggc tttggggggg ggctgtccct ctagagcccc atcctcactg
     8521 actccgtcct ggagttggat gagagataat ggccttacgt tgtgccaggg gagggtcggg
     8581 ctggatttag caagatttac cttctccaaa gagcggtgct gcagtggcac agctgcccac
     8641 ggaggtgggg gggtcaccgt ccctggaggt gatgaagaac tgtggggatg tggcactgag
     8701 ggacatggcc agtgggcacg gtgggtgggt tggggttggt cttggggatc ttggagggct
     8761 tttccagcct tcatgatttg acgattgtat gaacatctac atggcaattc tccagctgcc
     8821 tgtcccagtc ctactgaccc agctgtatct ctccaggcaa gctcttccac cccttctgct
     8881 tgcatccaga caccatcaaa catgcaggct cagacacagg gaccagcagt gtctgtggcc
     8941 tttttgtgct cctctccatg ctgggtttta acttgctctt tgtccttcta tcctatcttc
     9001 ttatccttaa ggctgttctg aacgctgtga cttggagagt gtcccagagc cctcaacacc
     9061 tgcatgtccc acgtccatgc tgtcctgcac ttccttatcc ccaagatctg cctctccgtg
     9121 atgcactgaa ttggcaaaca tgtgtcaccc cagaccaaca atgtcacagc aaactccccc
     9181 ttgataggac aagggggaat ggctttacac tgagacaggg gaggtttggg ttggatatga
     9241 ggaggcagtt tttcccccag agggtggtga cgcactgaac aggttgccca aggaggctgt
     9301 ggatgcccca tccctgcagg cattcaaggc caggctggat gtggctctgg gcagcctggg
     9361 ctgctggttg atgaccctgc acatagcagg gggttggatc tggatgagca ctgtgctcct
     9421 ttgcaaccca ggccgttcta tgattctgtc attctaaatc tctctttcag cctaaagctt
     9481 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     9541 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     9601 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     9661 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct ctagaagtcg
     9721 acggcgcgcc gtaaaagctt ggcatagatc ttattgcaga agccatatgt tttaggtgcc
     9781 agggcgtgcc cttgggctcc ccgggcgcgt ttgggcggcc gcatcgaatt ctaccgggta
     9841 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     9901 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     9961 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
    10021 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
    10081 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
    10141 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
    10201 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
    10261 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
    10321 tctccgggcc tttcgacctg caatcgccgc tagccgccac catgaaaaag cctgaactca
    10381 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    10441 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    10501 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    10561 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc agcgagagcc
    10621 tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    10681 aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc
    10741 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    10801 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    10861 acgacaccgt cagtgtaagt gctagcccct aggcctgaat cgaagttcct attcttcaaa
    10921 aggtatagga acttcgagct cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca
    10981 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
    11041 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
    11101 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
    11161 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
    11221 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    11281 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    11341 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    11401 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    11461 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    11521 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    11581 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    11641 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    11701 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    11761 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
    11821 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
    11881 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
    11941 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
    12001 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
    12061 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    12121 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    12181 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    12241 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    12301 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    12361 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    12421 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    12481 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    12541 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    12601 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    12661 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    12721 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    12781 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    12841 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    12901 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    12961 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    13021 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    13081 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    13141 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
    13201 cgtc
//
