LOCUS       sequence-299977-        1476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of Cas9 and sgRNA targeting SLC35B2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Zhang Q, Xu Y, Lee J, Jarnik M, Wu X, Bonifacino JS, Shen J, Ye Y
  TITLE     A myosin-7B-dependent endocytosis pathway mediates cellular entry of
            alpha-synuclein fibrils and polycation-bearing cargos.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 May 19;117(20):10865-10875. doi: 
            10.1073/pnas.1918617117. Epub 2020 May 4.
  PUBMED    32366666
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1918617117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-05-19- 19"; volume: "117"; issue: "20"; pages: 
            "10865-10875"
COMMENT     Sequence Label: Lenti-SLC35B2-sgRNA
FEATURES             Location/Qualifiers
     source          1..1476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(26..237)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(299..374)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(396..644)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(454..473)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(624..644)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    695..812
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1111..1155)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     gap             1342..1351
                     /estimated_length=10
     gap             1365..1374
                     /estimated_length=10
ORIGIN
        1 tggtggcagc gctctagaac cggtcctgtg ttctggcggc aaacccgttg cgaaaaagaa
       61 cgttcacggc gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga
      121 gccagtacac gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggatc
      181 aattgccgac ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact
      241 gacgggcacc ggagccaatt cccactcctt tcaagaccta gctagcgaat tcaaaaaagc
      301 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
      361 tctagctcta aaacggtgca gtacttcagg cggacggtgt ttcgtccttt ccacaagata
      421 tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta
      481 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt
      541 tgtactaata tctttgtgtt tacagtcaaa ttaattctaa ttatctctct aacagccttg
      601 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcggtacc ttaattaacc
      661 aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt
      721 tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac
      781 tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtctgc
      841 agaattggcg cacgcagtgc cgatccgttc actaatcgaa tggatctgtc tctgtctctc
      901 tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tggggagggg
      961 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     1021 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     1081 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     1141 ttcttgttca ttcttttcct ggcggnttgg cgattcttca attaaggnng gnnntaagct
     1201 tgggnaangn ttaatttccn nggccnnntc catccaggtc ggggnattcc aaancgnttc
     1261 caaaantttt nnnccnnact nncnttcnnn ggcnannnan gggggncnaa nnnntttncc
     1321 naaaaanccc naantccccn gnnnnnnnnn naaccttngn nnttnnnnnn nnnngnnntt
     1381 nggngggnaa gnnnnnnnnc ccnaangngn nnnngnnaaa annnnnnnng ccnnnnnaan
     1441 ccccccnaaa ntnnnnnnnn ngnccnannn naaaaa
//
