LOCUS       sequence-299988-       11198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing flag-tagged MIB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Bauer M, Flatt JW, Seiler D, Cardel B, Emmenlauer M, Boucke K, 
            Suomalainen M, Hemmi S, Greber UF
  TITLE     The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome 
            Release at the Nuclear Pore Complex.
  JOURNAL   Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 
            10.1016/j.celrep.2019.11.064.
  PUBMED    31851912
REFERENCE   2  (bases 1 to 11198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.11"; journalName: "Cell Rep"; date: 
            "2019-12-17- 17"; volume: "29"; issue: "12"; pages: "3785-3795.e8"
COMMENT     Sequence Label: pLVX-Flag-MIB1-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    440..565
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1787..1902
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        1960..2263
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2264..2467
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2418..2438
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2464..2488
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2571..2636
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(5707..6281,6282..6284,6285..6293)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5874..5891)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6101..6120
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6313..6912
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6313..6332)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6809..6829
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6926..7514
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6979..6999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7397..7408)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7721..8354
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8482..8498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8482..8498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8495..8517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8506..8522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8530..8536,8537..8554,8555..8560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8575..8596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8713..8730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8884..9472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8964..8983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9643..10434,10435..10503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10266..10285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10504..10608)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10656..10790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10693..10712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10747..10766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10958..11198,1..393)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
       61 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      121 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      181 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      361 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      421 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      481 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      541 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      601 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      661 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      721 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
      781 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
      841 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
      901 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     1801 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1861 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1921 cagcagagat ccagtttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     1981 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2041 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2101 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2161 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2221 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2281 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2341 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2401 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2461 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2521 ccatagaaga caccgactct actagaggat ctatttccgg tgaattcatg gactacaaag
     2581 accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagggtg
     2641 gaggcggttc cagtaactcc cggaataacc gggtgatggt ggaaggggtt ggcgctcggg
     2701 tagtgcgcgg cccggactgg aagtggggga agcaggacgg cggcgagggc catgtgggca
     2761 ccgtccggag cttcgagagc cccgaggagg tggtggtagt gtgggacaac ggcacagctg
     2821 ccaactaccg ctgctccggg gcttacgacc tccgcatcct ggacagcgcg cccaccggca
     2881 tcaagcatga tggaaccatg tgtgatacct gccgccagca accaatcatt ggcattcgat
     2941 ggaagtgtgc agagtgtaca aattatgatt tgtgcacagt gtgttatcat ggagataaac
     3001 atcatttaag acatcgcttt taccgaatta ctacaccggg aagtgagagg gttctgttag
     3061 agtctcgtag gaaatctaag aagattacag ccagaggaat ctttgcaggt gccagagtgg
     3121 tgcgaggagt ggactggcag tgggaagatc aagatggagg aaatggacgt aggggaaagg
     3181 taacagaaat ccaggactgg agtgcatcaa gcccacatag cgcagcatat gtcctctggg
     3241 ataatggtgc taagaacctt tacagagttg gctttgaggg catgtctgat ctgaaatgtg
     3301 tccaggatgc caagggaggt tctttctaca gagatcactg ccctgtgcta ggtgagcaga
     3361 atggcaacag gaatcctggt ggattgcaga ttggtgacct ggtaaatata gatctcgacc
     3421 tcgaaattgt acagtctttg cagcatggtc atggaggatg gactgatgga atgtttgaga
     3481 ctttaactac aactggaact gtttgtggca ttgatgaaga tcatgacatt gtagtacagt
     3541 atccaagtgg caataggtgg accttcaatc ctgctgttct cactaaagcg aacattgtcc
     3601 gaagtggaga tgctgctcag ggtgcagaag gaggcacctc gcagtttcaa gtgggtgatc
     3661 ttgtacaagt ttgttatgac ctggaacgaa ttaaacttct acaaagagga catggagaat
     3721 gggctgaagc gatgcttcca actttaggta aagttggccg agtacaacag atttattcag
     3781 acagtgattt aaaggtggaa gtttgtggaa catcttggac atacaatcca gcagcagttt
     3841 ccaaggtggc atctgcagga tcagccatta gcaatgcatc tggtgaaaga ctctcacaac
     3901 tcctgaagaa attatttgaa acccaagaat ctggtgacct caatgaagaa ttagttaagg
     3961 ctgctgccaa tggagatgtt gctaaagtgg aagatttgct taaaagacca gatgtggatg
     4021 taaatgggca atgtgctggc cacacagcta tgcaagctgc tagtcagaat ggacatgttg
     4081 acattttgaa gttacttttg aagcaaaacg tggatgtcga agcagaggat aaagatggtg
     4141 atagagcagt tcaccatgca gcttttggag atgaaggcgc tgttatagaa gtactacatc
     4201 gaggtagtgc tgatttgaat gctcgaaaca agcgccgaca gacaccactt catattgctg
     4261 tcaataaagg tcatcttcaa gttgtgaaga ctttattgga ctttggctgt catcccagtc
     4321 tccaggattc tgaaggtgat acccctcttc atgatgcaat aagtaagaaa cgtgatgata
     4381 tcctagcagt tcttttggaa gctggagcag atgttaccat cacaaacaat aatggattta
     4441 atgctctgca tcatgctgca ctaaggggaa atcccagtgc aatgcgtgtt ttactatcta
     4501 aattaccaag accatggatt gtggatgaga agaaagatga tggttatact gccttacatc
     4561 tggctgccct taataatcac gtagaagtgg ctgaactgtt ggtacatcag ggtaatgcaa
     4621 acctggatat ccagaatgtg aaccaacaaa ctgccctaca ccttgctgtt gaacgacagc
     4681 atacccagat tgttaggctt ttggtccgtg caggtgccaa gcttgatatt caggataagg
     4741 atggggatac tcctttgcat gaagctctaa ggcatcacac tttgtctcag ctacgtcagc
     4801 tccaagatat gcaagatgtg gggaaggtgg atgctgcctg ggagccatcc aaaaacacgt
     4861 taataatggg acttggtacc cagggggcag agaagaagag tgcagcatct attgcctgtt
     4921 tcttggcagc caatggtgct gacctgagca ttcgaaataa gaagggtcaa tcgccacttg
     4981 atctctgtcc tgatccgaat ctctgcaaag cactggcaaa gtgtcataag gaaaaagtca
     5041 gtggtcaagt gggttctcgg agtccttcta tgattagtaa tgattctgaa accttagaag
     5101 agtgtatggt gtgctcagat atgaagagag atactctttt tggtccatgt ggacatattg
     5161 ctacctgttc tttatgttct ccacgtgtca agaaatgcct catctgtaaa gaacaggttc
     5221 aatccaggac aaagattgaa gaatgtgtgg tatgctctga caagaaagca gctgttcttt
     5281 ttcaaccctg tggccacatg tgtgcttgtg agaactgtgc taacctgatg aaaaagtgtg
     5341 tgcagtgtcg agcagtagtt gaacgaagag tgcctttcat tatgtgctgt ggagggaaaa
     5401 gttcagaaga tgccactgat gatatctcaa gtgggaatat tccagtatta caaaaggaca
     5461 aggataatac caatgtcaat gcagatgtgc aaaagttgca gcaacagtta caagacatta
     5521 aagagcagac aatgtgccct gtgtgtctag atcgtctgaa gaatatgatt ttcctttgtg
     5581 gtcacggaac ctgtcaactc tgtggagacc gcatgagtga atgtcctatc tgtcgcaagg
     5641 ctattgaacg aaggattctt ttgtattaaa gtaaagaaag ggctaattcc gcggccgcgg
     5701 atcccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     5761 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     5821 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     5881 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     5941 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     6001 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     6061 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     6121 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     6181 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     6241 ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccaca acccacaagg
     6301 agacgacctt ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6361 ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6421 gtcgacccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6481 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6541 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6601 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6661 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6721 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6781 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6841 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6901 cccggtgcct agacgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt
     6961 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7021 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7081 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7141 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7201 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7261 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7321 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7381 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7441 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7501 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc
     7561 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     7621 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     7681 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc
     7741 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     7801 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     7861 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     7921 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     7981 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     8041 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     8101 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     8161 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8221 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8281 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8341 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8401 gaaatgaata tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag
     8461 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9301 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9421 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9481 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9541 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9601 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9661 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9721 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9781 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9841 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9901 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9961 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10021 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10081 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10141 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10201 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10261 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10321 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10381 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10441 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10501 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10561 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10621 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
    10681 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10741 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10801 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10861 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10921 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10981 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    11041 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    11101 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    11161 ttacaccctg tgagcctgca tgggatggat gacccgga
//
