LOCUS       sequence-299990-       11787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline 
            treatment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11787)
  AUTHORS   Bauer M, Flatt JW, Seiler D, Cardel B, Emmenlauer M, Boucke K, 
            Suomalainen M, Hemmi S, Greber UF
  TITLE     The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome 
            Release at the Nuclear Pore Complex.
  JOURNAL   Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 
            10.1016/j.celrep.2019.11.064.
  PUBMED    31851912
REFERENCE   2  (bases 1 to 11787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.11"; journalName: "Cell Rep"; date: 
            "2019-12-17- 17"; volume: "29"; issue: "12"; pages: "3785-3795.e8"
COMMENT     Sequence Label: pLVX-BSD-tet-mEGFP-MIB1
FEATURES             Location/Qualifiers
     source          1..11787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..324
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    54..72
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     297..321
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      382..391
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(388..390,391..393,394..1104)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(433..454)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(694..713)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1041..1062
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4205..4416
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4437..4832
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4854..4910
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4911..5528,5529..5534,5535..5570,5571..5573,5574..5609,
                     5610..5612,5613..5648,5649..5657)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5684..6272
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5737..5757)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6155..6166)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6371..7004
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7132..7148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7132..7148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7145..7167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7156..7172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7180..7186,7187..7204,7205..7210))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7225..7246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7363..7380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7534..8122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7614..7633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8293..9084,9085..9153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8916..8935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9154..9258)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9306..9440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9343..9362)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9397..9416
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9608..10241
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10288..10413
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10910..11143
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11328..11372
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11635..11750
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgatatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
       61 cagtgataga gaacgatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
      121 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      181 cgtatgtcga gtttatccct atcagtgata gagaacgtat gtcgagttta ctccctatca
      241 gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      301 cgtttagtga accgtcagat cgcctggaga attggctagc agaattctta gtgaacgtca
      361 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
      421 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      481 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      541 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
      601 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      661 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      721 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      781 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
      841 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
      901 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      961 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
     1021 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     1081 ctcggcatgg acgagctgta caagtccgga ctcagatctc gagctatgag taactcccgg
     1141 aataaccggg tgatggtgga aggggtaggg gcaagagttg tcagaggccc ggactggaag
     1201 tgggggaagc aggacggcgg cgagggccat gtgggcaccg tccggagctt cgagagcccc
     1261 gaggaggtgg tggtagtgtg ggacaacggc acagctgcca actaccgctg ctccggggct
     1321 tacgacctcc gcatcctgga cagcgcgccc accggcatca agcatgatgg aaccatgtgt
     1381 gatacctgcc gccagcaacc aatcattggc attcgatgga agtgtgcaga gtgtacaaat
     1441 tatgatttgt gcacagtgtg ttatcatgga gataaacatc atttaagaca tcgcttttac
     1501 cgaattacta caccgggaag tgagagggtt ctgttagagt ctcgtaggaa atctaagaag
     1561 attacagcca gaggaatctt tgcaggtgcc agagtggtgc gaggagtgga ctggcagtgg
     1621 gaagatcaag atggaggaaa tggacgtagg ggaaaggtaa cagaaatcca ggactggagt
     1681 gcatcaagcc cacatagcgc agcatatgtc ctctgggata atggtgctaa gaacctttac
     1741 agagttggct ttgagggcat gtctgatctg aaatgtgtcc aggatgccaa gggaggttct
     1801 ttctacagag atcactgccc tgtgctaggt gagcagaatg gcaacaggaa tcctggtgga
     1861 ttgcagattg gtgacctggt aaatatagat ctcgacctcg aaattgtaca gtctttgcag
     1921 catggtcatg gaggatggac tgatggaatg tttgagactt taactacaac tggaactgtt
     1981 tgtggcattg atgaagatca tgacattgta gtacagtatc caagtggcaa taggtggacc
     2041 ttcaatcctg ctgttctcac taaagcgaac attgtccgaa gtggagatgc tgctcagggt
     2101 gcagaaggag gcacctcgca gtttcaagtg ggtgatcttg tacaagtttg ttatgacctg
     2161 gaacgaatta aacttctaca aagaggacat ggagaatggg ctgaagcgat gcttccaact
     2221 ttaggtaaag ttggccgagt acaacagatt tattcagaca gtgatttaaa ggtggaagtt
     2281 tgtggaacat cttggacata caatccagca gcagtttcca aggtggcatc tgcaggatca
     2341 gccattagca atgcatctgg tgaaagactc tcacaactcc tgaagaaatt atttgaaacc
     2401 caagaatctg gtgacctcaa tgaagaatta gttaaggctg ctgccaatgg agatgttgct
     2461 aaagtggaag atttgcttaa aagaccagat gtggatgtaa atgggcaatg tgctggccac
     2521 acagctatgc aagctgctag tcagaatgga catgttgaca ttttgaagtt acttttgaag
     2581 caaaacgtgg atgtcgaagc agaggataaa gatggtgata gagcagttca ccatgcagct
     2641 tttggagatg aaggcgctgt tatagaagta ctacatcgag gtagtgctga tttgaatgct
     2701 cgaaacaagc gccgacagac accacttcat attgctgtca ataaaggtca tcttcaagtt
     2761 gtgaagactt tattggactt tggctgtcat cccagtctcc aggattctga aggtgatacc
     2821 cctcttcatg atgcaataag taagaaacgt gatgatatcc tagcagttct tttggaagct
     2881 ggagcagatg ttaccatcac aaacaataat ggatttaatg ctctgcatca tgctgcacta
     2941 aggggaaatc ccagtgcaat gcgtgtttta ctatctaaat taccaagacc atggattgtg
     3001 gatgagaaga aagatgatgg ttatactgcc ttacatctgg ctgcccttaa taatcacgta
     3061 gaagtggctg aactgttggt acatcagggt aatgcaaacc tggatatcca gaatgtgaac
     3121 caacaaactg ccctacacct tgctgttgaa cgacagcata cccagattgt taggcttttg
     3181 gtccgtgcag gtgccaagct tgatattcag gataaggatg gggatactcc tttgcatgaa
     3241 gctctaaggc atcacacttt gtctcagcta cgtcagctcc aagatatgca agatgtgggg
     3301 aaggtggatg ctgcctggga gccatccaaa aacacgttaa taatgggact tggtacccag
     3361 ggggcagaga agaagagtgc agcatctatt gcctgtttct tggcagccaa tggtgctgac
     3421 ctgagcattc gaaataagaa gggtcaatcg ccacttgatc tctgtcctga tccgaatctc
     3481 tgcaaagcac tggcaaagtg tcataaggaa aaagtcagtg gtcaagtggg ttctcggagt
     3541 ccttctatga ttagtaatga ttctgaaacc ttagaagagt gtatggtgtg ctcagatatg
     3601 aagagagata ctctttttgg tccatgtgga catattgcta cctgttcttt atgttctcca
     3661 cgtgtcaaga aatgcctcat ctgtaaagaa caggttcaat ccaggacaaa gattgaagaa
     3721 tgtgtggtat gctctgacaa gaaagcagct gttctttttc aaccctgtgg ccacatgtgt
     3781 gcttgtgaga actgtgctaa cctgatgaaa aagtgtgtgc agtgtcgagc agtagttgaa
     3841 cgaagagtgc ctttcattat gtgctgtgga gggaaaagtt cagaagatgc cactgatgat
     3901 atctcaagtg ggaatattcc agtattacaa aaggacaagg ataataccaa tgtcaatgca
     3961 gatgtgcaaa agttgcagca acagttacaa gacattaaag agcagacaat gtgccctgtg
     4021 tgtctagatc gtctgaagaa tatgattttc ctttgtggtc acggaacctg tcaactctgt
     4081 ggagaccgca tgagtgaatg tcctatctgt cgcaaggcta ttgaacgaag gattcttttg
     4141 tattaaagta aagaaagggc gggatccttg aaaggagtgg gaattggctc cggtgcccgt
     4201 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     4261 tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     4321 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     4381 gttctttttc gcaacgggtt tgccgccaga acacaggacc ggttctacgt acgaccatgg
     4441 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     4501 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     4561 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     4621 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     4681 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     4741 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     4801 aattgctgcc ctctggttat gtgtgggagg gcatgcatag ccgcggcagc ggcgctacta
     4861 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct atgtctagac
     4921 tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag
     4981 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
     5041 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
     5101 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
     5161 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
     5221 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
     5281 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
     5341 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
     5401 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
     5461 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
     5521 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
     5581 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
     5641 acatgctccc cgggtaacta agtaaggatc gatccaagat atcaatcaac ctctggatta
     5701 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5761 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5821 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5881 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5941 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6001 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     6061 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     6121 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     6181 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     6241 gagtcggatc tccctttggg ccgcctcccc gcctgtttcg cctcggcgtc cggactagag
     6301 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6361 aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6421 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6481 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6541 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     6601 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     6661 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     6721 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6781 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6841 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6901 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6961 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7021 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     7081 acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7141 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7201 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7261 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7321 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7381 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7441 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7501 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7561 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7621 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7681 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7741 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7801 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7861 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7921 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7981 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8041 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8101 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8161 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8221 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8281 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8341 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8401 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8461 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8521 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8581 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8641 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8701 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8761 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8821 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8881 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8941 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9001 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9061 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9121 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9181 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9241 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9301 agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9421 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
     9481 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
     9541 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
     9601 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
     9661 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
     9721 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
     9781 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
     9841 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
     9901 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
     9961 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
    10021 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
    10081 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
    10141 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
    10201 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
    10261 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
    10321 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
    10381 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
    10441 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
    10501 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
    10561 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
    10621 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
    10681 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
    10741 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
    10801 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
    10861 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
    10921 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
    10981 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
    11041 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
    11101 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
    11161 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
    11221 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
    11281 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
    11341 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
    11401 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    11461 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    11521 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    11581 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgcctttaaa
    11641 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
    11701 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
    11761 tacagggaca gcagagatcc agtttat
//
