LOCUS       sequence-300007-         106 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a 
            Direct Repeat (DR) fragment into the pLCHKO hgRNA vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 106)
  AUTHORS   Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, 
            Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, 
            Myers CL, Blencowe BJ, Moffat J
  TITLE     Genetic interaction mapping and exon-resolution functional genomics 
            with a hybrid Cas9-Cas12a platform.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):638-648. doi: 
            10.1038/s41587-020-0437-z. Epub 2020 Mar 16.
  PUBMED    32249828
REFERENCE   2  (bases 1 to 106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0437-z"; journalName: "Nat Biotechnol"; date: 
            "2020-05"; volume: "38"; issue: "5"; pages: "638-648"
COMMENT     Sequence Label: Topo_SpCas9.tracr_AsCas12a.DR
FEATURES             Location/Qualifiers
     source          1..106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atctacaaga gtagaaatta gcaccgactc ggtgccactt tttcaagttg ataacggact
       61 agccttattt caacttgcta tgctgtttcc agcatagctc tgaaac
//
