LOCUS       sequence-300086-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  transfection plasmid for the exogneous expression of C-terminal 
            miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for 
            BioID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Aregger M, Lawson KA, Billmann M, Costanzo M, Tong AHY, Chan K, 
            Rahman M, Brown KR, Ross C, Usaj M, Nedyalkova L, Sizova O, Habsid 
            A, Pawling J, Lin ZY, Abdouni H, Wong CJ, Weiss A, Mero P, Dennis 
            JW, Gingras AC, Myers CL, Andrews BJ, Boone C, Moffat J
  TITLE     Systematic mapping of genetic interactions for de novo fatty acid 
            synthesis identifies C12orf49 as a regulator of lipid metabolism.
  JOURNAL   Nat Metab. 2020 Jun;2(6):499-513. doi: 10.1038/s42255-020-0211-z. 
            Epub 2020 Jun 1.
  PUBMED    32694731
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-020-0211-z"; journalName: "Nat Metab"; date: 
            "2020-06"; volume: "2"; issue: "6"; pages: "499-513"
COMMENT     Sequence Label: pCDNA5_C12orf49-miniTurbo
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..771
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
ORIGIN
        1 atcccgctgc tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc
       61 ctgcctgtgg tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt
      121 ggggatgctt gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg
      181 ttctctcctt ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca
      241 gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg
      301 ggagcagaca aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg
      361 gcaggcatcc tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc
      421 gccgggatta acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc
      481 acactgcagg aagcagggat taacctggac aggaatactc tggccgctat gctgatccga
      541 gagctgcggg cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg
      601 tgggagaagc tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag
      661 attttcggga ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga
      721 gttatcaaac cctggatggg cggagaaatc agtctgcggt ctgccgaaaa g
//
