LOCUS       sequence-300101-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression and labeling mitofusin 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abrisch RG, Gumbin SC, Wisniewski BT, Lackner LL, Voeltz GK
  TITLE     Fission and fusion machineries converge at ER contact sites to 
            regulate mitochondrial morphology.
  JOURNAL   J Cell Biol. 2020 Apr 6;219(4). pii: 133843. doi: 
            10.1083/jcb.201911122.
  PUBMED    32328629
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-04-6"; volume: "219(4). pii: 133843. doi"; 
            pages: " 10.1083/jcb.201911122"
COMMENT     Sequence Label: mCh-Mfn2
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     gap             2..20
                     /estimated_length=19
     regulatory      68..77
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             74..781
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(219..237)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     487..506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cnnnnnnnnn nnnnnnnnnn agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
       61 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
      121 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      181 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      241 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      301 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      361 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      421 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      481 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
      541 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      601 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      661 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      721 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      781 gtccggactc agatctcgag tgatgtccct gctcttctct cgatgcaact ctatcgtcac
      841 agtcaagaaa aataagagac acatggntga nnngaatgca tccccactta agcactttgt
      901 cactgccnag aanaagatca atggnatttt tgagcagctg ggggnctaca tccagnagag
      961 cgccnccntn cnngaagann ngtacaggaa tgcnnaactg gancccnnnc nnnnananng
     1021 gnncngnngt nannnncnnt cnnnngnngn atcnnnnngn nnnnngnncg agnnnnngaa
     1081 nnngcttttt ttnnnnnnnn ancnngggnn nnancnccnn nnannaatg
//
