LOCUS       sequence-300131-       14878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, 
            with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) 
            sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata 
            S, Ikawa M, Okano H
  TITLE     Developmental analyses of mouse embryos and adults using a 
            non-overlapping tracing system for all three germ layers.
  JOURNAL   Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 
            10.1242/dev.174938.
  PUBMED    31597657
REFERENCE   2  (bases 1 to 14878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2019-11-4"; volume: "146(21). pii: 
            dev.174938. doi"; pages: " 10.1242/dev.174938"
COMMENT     Sequence Label: Sox1(2A-Cre)
FEATURES             Location/Qualifiers
     source          1..14878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4491..4547
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4551..4571
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4575..4595
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4596..5627
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     protein_bind    complement(5633..5666)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    5672..5720
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(5727..6326)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5810..5830)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6307..6326
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(6345..6392)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6404..6903)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6452..6471)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     6845..6868
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(6966..6999)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(12495..12511)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12495..12511)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12508..12530)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12519..12535
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12543..12549,12550..12567,12568..12573))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12588..12609
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12668..12863
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12714..12849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12869..13003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12906..12925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12960..12979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13071..13088)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13242..13830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13322..13341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14001..14792,14793..14861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14624..14643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaggtctggc acaccatgcc caccactgct tagttggcac agactgcaac caccaatctt
       61 ctctgtcact tggcaacagg ttacacctgc atctctgatt tcatacttct gcaggtgggc
      121 acccataggt tttgcaccag cttggacttg cttctgcaga atagttccca gaatgccaaa
      181 caccaacatg gcagtatgca ttgtgatgcc gtttacctcc tatttagatg tcctagaaaa
      241 caagagtaac atcctatttg gaaacaatgt tcagccctat ggtaggaaga aatgagactg
      301 tttcaaagtc atctatccaa gtttcctaag gcataaagca ttcctgcaaa gctcactgga
      361 gtgcagggtg tttataaaat aaattgcccc attgatatgg aaaataatgt gccaggcttg
      421 atttacacaa agcattttca gggggtcttt ggctatttag tttaaaatct aaattgaaaa
      481 gcacaagaaa gaaatctttc aattgctaag tggataatat agtttataat ttgtaaaccg
      541 tttcctttat ttcagtcttg ctgacttgcc ctaacacagc ggatctccca gcacacccat
      601 cacctcctaa gttatccaag gaaaatggtt ctacggcatt cttaaacaat agtcctattc
      661 gtttttgttc atttacaatg taactgatat ttgtaggtag ttcgtgaaac aaatcaaaag
      721 atttaataaa cttgatggct gaaataagaa ggtagctgaa aactaagttt atgacctaga
      781 tgtttataaa aatatgaaac gatgtttccc ttctagcaca attctatatt atttgatgag
      841 ttttctagta agtgctgaat ggcctgaaat gaaagctcaa tcgggctgag ggctgtactg
      901 tgctgtaaca acctgagata aaaaaaaata ataataaaat aaaataaacc actgtaataa
      961 tcatagcaag ggagcaacgg cgtcaggaaa ttcacagcct cagatgtttt tgttagtggg
     1021 ggggggggct gtcatatgtt caccgtccaa catattggaa cacctgtatt cttgctatta
     1081 tccgggttat ctgtcatcag aagagcctct ggattacacg cgaggcggtc tgcaggcatc
     1141 tgaccaggct tgctttaccc accagcaacc tcccgaagca cctagatctt agaagtagaa
     1201 actctatcct ctcccctctg taccccgccc caggatatct gaagaacttg tagactctaa
     1261 aggtctttct ggaagaagcc tttacaataa ctcccccccc cttttttttt ttggacatga
     1321 tggtctgatc ccaagtagcc tggggtgggg ttggaaatct cttatacatc ccggaatcat
     1381 atttgttctg agattcatgg attacggcca tttgtgtatg tcttgatcat ccccagaact
     1441 gccctttctt tttcacaaca tacgaagaaa caaaaacaac aacatacaaa aaggcggccc
     1501 tgtcactgga ccttccaatt ccgtttcagc gcccgcaccc cacccccacc ctcgcaaacc
     1561 cgagttcaca cgattttcaa aatcctttgg caaacagctt ctgggagaat cgcttcagaa
     1621 aagcataggc taggtaggta ttcttttgct gtttccacaa acttcttttt actgtcggag
     1681 taacaagatt ccagctagat cttggttttg accttcgcag aaattcaaga ggatatagag
     1741 taaattggca cctgactata acactccgct ccattaaaag acaccccctc ccccaagaaa
     1801 gttcggggta gattagggag ctaggagcgt agcaccacac aaaaaccgct gcacctcttg
     1861 ccacggagac ccgaattata gatcccgaat ctgtaaaggc ctttgcaggc tctgagaatg
     1921 caccgagcgc caggggtggg agagactgag ccgccccgct tggcctgaaa cccagcagag
     1981 gtactttggg cctaagcccc ccccccccgg gaggatctcc cccgcaccgc tgttattctg
     2041 cactcccggc tgagctgagt gcaaagtgag gcctctatct ccaactttcc ccttcccagc
     2101 gggcacagag ttggctggct ggggacaggg gaggagctgg gtctccaatg agcgcattta
     2161 aacacgaccc agggctgtat tccccccgcc tcccccgaca gcgattcttt cttcctctgg
     2221 gttttcaacc caatcgttaa tcattcggag cgcgcgggcg gggagaggca aagggggcga
     2281 gctctgggtt cgccgccgcc gccgccgccg tcgccgccgc gcgcgcgcgc tcaggaagca
     2341 gtgtggctgt gaccccgccc ccccgcgccc ccttttcccc ctccttcctg cccttgttcc
     2401 ccccctctct cctcctccac ctccttgctc cgccgcccgc gcccagtgta tctactccct
     2461 ccccacgtca ctcgccagcg cgccatgcaa atcaccgccg ccgccggttc ccattggccg
     2521 cggcgcgctc atttaatggc agcccgggcc cggcgtatgg ctgctgggcc ccccgcgcca
     2581 ccggccccgc gtgcgcctcc gctccgagcg cacggcctcg ggcaggcagc ggacagcttg
     2641 tcctgggcac agcggcacag ctctctggct ctctgctcgc gttggcactc ccactgctgt
     2701 ccctaacccc ctccctggct gcaccgcgat cgccccctcc gaacccccct ccgagtcccc
     2761 ctcagcgcct ggctcccccc aggcacctgg gaccagcaca tgcccagcgc acgcggcgca
     2821 ccgccttgct agaagttgcg gtcccagagt tggaagccgc caaggaagcc agcacaggaa
     2881 cggagacttc gagccgacaa gaggaggcag cgaaccctgc gtcgggccca ggggcaccgc
     2941 ttcagaccca gaaagtggag cctcaacttg gccacgactg cacctgtttg cacagttcag
     3001 ccctgagtga ccggacggca gcaatcaacc tggccatcgg cctctttggc aagtggtttg
     3061 tgcaccggga gaaactttcc acctgcgagc tggacccgcg ctaagtgcgt gtgcttttgc
     3121 ctcttttttg ttgttgttgt tgttgtggcc tccacccaac ccccttctct ccgctaggca
     3181 cccaccgcac acacaccccc ccccccagtc tctctgggct gatcctctct ccacccaccc
     3241 acccccaccc ggccgtctat gctccaggcc ctctctttgc ggtaccggtg aacccgctag
     3301 ccgcccagat gtacagcatg atgatggaga ccgacctgca ctcgcccggc ggcgcccagg
     3361 cgcccacgaa cctctcgggc ccggccgggg cgggcggggg cggcggtggg ggcgggggcg
     3421 gcggcggcgg cgggggcacc aaggccaacc aggatcgggt caagcggccc atgaacgcct
     3481 tcatggtgtg gtcccgcgga cagcggcgca agatggccca ggaaaacccc aagatgcaca
     3541 actcggagat cagcaagcgc ctcggggccg agtggaaggt catgtccgag gccgagaagc
     3601 ggccgttcat cgacgaggcc aagagactgc gcgcgctgca catgaaggaa cacccggatt
     3661 acaagtaccg gccgcgccgc aagaccaaga cgctgctcaa gaaggacaag tactcgctgg
     3721 ccggcgggct gctagcggcc ggcgcgggtg gcggcggcgc ggccgtggcc atgggtgtgg
     3781 gcgtgggcgt cggggcggcg gcggtgggcc agcgcttgga gagcccaggc ggcgcggcgg
     3841 gaggaggcta cgcgcatgtc aacggctggg ctaacggcgc ctaccccggc tcggtggccg
     3901 cggcggctgc ggccgcggcc atgatgcagg aggcacagct ggcctacggg cagcacccag
     3961 gcgcgggcgg cgcgcacccg cacgcacacc cggcgcaccc gcacccgcac catccgcacg
     4021 cgcatcctca caacccgcag cccatgcacc gctacgacat gggcgcgctg cagtacagcc
     4081 ccatctccaa ctctcagggc tacatgagcg cgtcgccttc gggctacggc ggcatccctt
     4141 acggcgccgc ggccgccgcc gccgccgctg cgggcggcgc gcaccagaac tcggcggtgg
     4201 cggcagcggc agccgcggca gccgcgtcgt cgggggccct gggcgccctc ggatctctgg
     4261 tcaagtcgga gccgagcggc agtccgccgg ccccggctca ctcacgggca ccgtgtcccg
     4321 gggacctgcg cgagatgatc agcatgtacc tgcccgccgg cgagggtggc gacccggcgg
     4381 cggcagcggc tgcggcggcc caaagccggc tgcactcgct gccacagcac taccagggcg
     4441 cgggcgcggg cgtcaacggc acggtgcccc tgacgcacat cggaagcgga gctactaact
     4501 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgat ccaaaaaaga
     4561 agagaaaggt agatccaaaa aagaagagaa aggtaatgtc caatttactg accgtacacc
     4621 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
     4681 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
     4741 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
     4801 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     4861 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     4921 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     4981 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
     5041 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
     5101 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
     5161 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
     5221 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
     5281 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
     5341 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
     5401 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
     5461 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
     5521 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     5581 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattagcca ttgaagttcc
     5641 tatactttct agagaatagg aacttcgatc cgaacaaacg acccaacacc cgtgcgtttt
     5701 attctgtctt tttattgccg atcccctcag gcaccgggct tgcgggtcat gcaccaggtg
     5761 cgcggtcctt cgggcacctc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     5821 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     5881 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     5941 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     6001 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     6061 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     6121 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     6181 agctcggtga cccgctcgat gtggcggtcc ggatcgacgg tgtggcgcgt ggcggggtag
     6241 tcggcgaacg cggcggcgag ggtgcgtacg gccctgggga cgtcgtcgcg ggtggcgagg
     6301 cgcaccgtgg gcttgtactc ggtcatggtt tagttcctca ccttgtcgta ttatactatg
     6361 ccgatatact atgccgatga ttaattgtca acaggctgca ggtcgaaagg cccggagatg
     6421 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
     6481 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     6541 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     6601 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
     6661 gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
     6721 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
     6781 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
     6841 tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta
     6901 cccggtagaa ttgacctgca ggggccctcg atagcttggc tggacgtaaa ctcctcttca
     6961 gacctgaagt tcctatactt tctagagaat aggaacttcc gccgcgggga cgccggggac
     7021 actgcggctt aaggccggcg ccccggcgac gaagagcgag gcctgcgccc cagcctccag
     7081 agcccgactt tgtaccgagg tccccgcgct ctcgataaaa ggccgctctg gagagccgag
     7141 cgccaggtga catctgcccc catcaccttc cccaggactc cgaggcgctg acaccagact
     7201 ggcctcttag actgaacttt ggtgttttca tgagaccttt tgtacagtat ttatcgtccg
     7261 cagaggaggc acacagcgtt ttctcggctt cggaggacaa aagacaaaaa cccagcgagg
     7321 cgatgccaac ttttgtatga ctgccggctc tgtaactttt tccggggttt acttcccgcc
     7381 agctcttctg cctgaggccg agtgacggac ctcgagccct tctcacttgt tataaatcta
     7441 agtaaggcag atccaaacat ttacaagttt tttgtagttg ttaccgctct tttgggttgg
     7501 ttggttaatt tataccgcaa tcccctctca gacggtggag ttatattctg ggttttgtaa
     7561 atctctgtat ccgagcattt ccaatttttt gttttgtttt gtattatttc ttgtaaatgc
     7621 gttgtgacat ttttatttta ggcgttgcga tacgggggga agaggagtcg gatgttgtac
     7681 atagcctgca agtctttcat ctaaaagcaa aaaacaaaga gagatacccc caaaatgcat
     7741 caaatttgaa tcaatacatt taagagagaa aagcccgttt ccctgtgaaa tcgaaacgtg
     7801 ctaattttat atgcatgttt tatgagttca aaatacaatt agcaatctgt tggaggagaa
     7861 attatctgca gggcccaaga gtaaggaaat cccaccaagc ggagtgcagc gtttccgtga
     7921 ctttattcgg tggggaaaga gaaatcttaa actccataga ttattttgta gagttcaagt
     7981 tgacacagaa gggaagtaat tacaaaatgc ttcctaatgg tttagcgttt taactgccat
     8041 ggccctggct tatttttttt tgcatttctt tcctgtggtt ctgccttttg catactgtag
     8101 cagttttgag gcagctgggt ctcagaagga ggatgggttg tgctcagtgg tgggacaagg
     8161 ccaaggatag ggacaacagc caccgccaag acagcgtgcc tttgatttct ctgtaattgg
     8221 aattgtaagt gaaattatct tcctgtgctt caaggaagca atcctgaccc agacctcacc
     8281 accacagagt ctctcaccca ggtcttatcc catcccctcc cgattcctct ctgggaaaat
     8341 ccaacataat tctaccgatt ttcttacaca cacacacaca cacacacaca cacacactct
     8401 ctcctgtgtg aaccgggatg cagattgcca gggaatgtaa acacagaatg ctgtgtttat
     8461 cattcctgac cagatcttga ggttgtttga tgctttaaat atttttaatt atattttttc
     8521 taggtgttta ttggtatatt gcaggtcccc cccccctgga atttaaagtt ccctgtaaaa
     8581 ctttgtcttc aagtaatctg acagcattaa atattgcatt taaaagttat actgtagcaa
     8641 atatgtttta aaattaatca tgacattaga atgaaattct atttttgaag gaaattttct
     8701 ttaagtccag atggggaatg atactatttt cagcatcctt tcccctgatt ttttcccaat
     8761 ttcttttctt ggaatgaata tggactagcc attcaaaagt ggacttaatc tagcaattca
     8821 gtaagaccag agctcaacct acattggaga atatctttca gtataaatgc taatattaca
     8881 agtgacacgt gtgatttttt ttaaagtcat ttaatgattt tgcttaaacc cctcattcct
     8941 taaagtgatt gcaagagatt caagaatgac gcccactgct ttctgatgag gtaataaaag
     9001 aacacccctc cctttttcct ttctttgttt ttcttttagg gttttgcttt gttgtttcta
     9061 gagctgctta gcctaccgct taggagtcca ggagggaggc cacactctgt ggcagtctag
     9121 gaagaaatgc cccaatagcc tcagggaaat tctagagagt gtttagagtc caacccactt
     9181 cccccttaaa acgccaagac ctttgcagac tggcccccaa caccaccctg ctctttattt
     9241 agggagaatc aaaggtggcc acactcacct ctgttttgcc tgggcttctg gagttcctgg
     9301 tgtgccttgg ctgggggcaa ctaaaccagg gctcagtgca taggagttat ggccacttta
     9361 agtgaagcac cccacataaa gttagtcatc ttgttagtat cagacagctc agcccccacc
     9421 cccaccccaa gactttcaga ggtttgtgct ccctacttga gctctaccca gtgaacaaga
     9481 actctttgtg ccaggctttc aagccacctt cctttgggtt ctactgatgt gaaagacaag
     9541 gagaaattct ctaatgactt aatttgaaaa caatacagag gggggggggc ttctctcaag
     9601 atcgaggcca gatccagaca gacaaacttt ctccatttta aataaatgga gagaattttc
     9661 tcctaggtct tacttagagc ccaactcaag ggtagtttca gtaccttacc gagccatgca
     9721 catttttatt caagtgaggg ttttttgttg ttgttgtttt tgtttttttt ttttatggct
     9781 atgattacca gataactcac ttccaaagtt cgggcggtgt ttccttctat tagtgcgatc
     9841 ttgtttaaaa ctctgtgtgg agaatggggc aggatctttg cgaagcgtgg ataaagtttg
     9901 cataacttcg tgtagggcgc ctgggtggct tctaacccac gggtaaactg agcgggatgg
     9961 tttgcctggt ggtcgccccc cctccgcccc cttgcccctc tccctccctc tctcctattc
    10021 ccctttcttt gctcacttaa aaaaaaaaaa ctcactggcc cctttttatt gttgccactg
    10081 agactttgac accctccctc aaacgcacta gtaggcagat gaaacgaatt aattttttta
    10141 aaggagttgc gggcgcagcg cgcctgatct gcagatgtgt cgggatgtga ctgataggtc
    10201 tattaaaatc atgcgtgtgg gagaagaata acactcatat tcacgcattt cattttcccc
    10261 gacaaagact gggcactgcc gagctctgtc tccggaaaat tccagtttat tccagaagcc
    10321 ctccccagca ctcatgttct ggggacgcag gaaagcaagc tctgtccagc ccgggttcag
    10381 cactgctggc tcggggcccc tgagctcttg tggtcacgcc ctctccccaa cccagcttcc
    10441 gcagcgggtg tccgggagcc ccgcggtgga atctaaccgt aggttagagt cctgggaaag
    10501 ttcaggagga gagcagactt ggccttggcg cagcacttgg cgggattgag agggcggacg
    10561 gataaggaag agagaggcgc agcgcgggcc cccggggcga gaatgccaga gtccctctgt
    10621 gcggggtggc gggtggcggg tggactggtc caggctcctg cagcgcagct caagcctgcc
    10681 tccccagctc gaagctggga agcctcacca cccctccttc tcttctcagc cctcctcttc
    10741 ccccctcccg cccggccctt cctctccagc tgtgcctggc aggacccagc ttcacccgcc
    10801 cccagaacca gcggggcagt gcggcggcgg acagcatcct ccgtcccagg cattgaggcc
    10861 aggacctctg ggggagcggg ccggcctcca gtggatcttc cacgctgcct cctgagttcc
    10921 aagtcacccg tgcttctgtg gttctgctgt ccgctgagac ccgcaccttg ctctgctctc
    10981 atgtccctct caccactttc ctgtccacca agtcttctca tccctgccct tcccctagcc
    11041 tccggtctgc agcctctgct cttgcttggc ttttccacca cgcgctgcca agccctccct
    11101 ttgtcttggt attcggtccg cttcccgcct cctcagcccc tctcttcccc agccgcccca
    11161 tcgtaggcac aggttcaggg cgatcggtgc tttcttccag cttgtctgtc cttgcggggg
    11221 tggcaagtaa gggcttggcg tccaccgtac tcatgcccac gcttgcccga aggccgttgg
    11281 ggaattaaga aaagagcggt gtgtgttaaa gactgcaggg ttatggcgga gaaagtcctg
    11341 acctggggtc agcctcaagc caaacccttt tacaatcagt cgggaaacca agcaggtccc
    11401 aggcgggacc gcaacaagtg ggcgcagaga gggggagggg aagcagggtt tccaggcaag
    11461 caaagacaag gaaaagacag agtggcaagc agctccaggc agaactgggt tctgggcaac
    11521 agcggacaca ggctgcgtct aggcctggac agtgactagc taccttcaaa ggtttcgttc
    11581 tcagggaacc gtctgcactg gctaagtcta gggtgcctcc tgggtactat tccttgggat
    11641 tcgagtctaa agagaaagct gcacgggcct ctacccaaga ggtcccgttt atttgagtag
    11701 caatacataa ataaataaaa ataagggcaa gttcttggaa gctggactca gtggtcctct
    11761 cgtaacccct gcattatttt agactcagta cagccatccg tgtctttgca agctgtagat
    11821 gagatggtat tgaaaggaca gtgtctttgc aagctataga tgagatggta ttgaacggaa
    11881 ggcattacta agacagttta gtcgccagac ttggttgagt gaaatactaa agggcaaaat
    11941 ttactattta ttcacgttga tggagaccag gaacgtggag ggaggcctga ggacagggaa
    12001 ataggtttgt cctccttagc gccccattgg gaattcctac tgagatgata tcggttaact
    12061 gataatgttt gacaaatgtg gaaaatataa aagcccaagg aagtaatata cacgcacaga
    12121 caaagagcct atgtttgggt aggacaaata aaaaacacaa aattgccaaa tcttctaaat
    12181 tctcacctgc acatgaacag actccaccag cagggacagg acagagccag ttttcagcgt
    12241 ttctttgttt ttgttttaaa taacaaactc gcacatccct gcacagtggg cgcaccctgt
    12301 ggactcacac acttaggtag aaagtaggct gtgagttcga agttcaaagt ggaagagcag
    12361 aatgctgcag aagcaaatgc taagtgacct tctggggaaa tttgcctgtg tcagagacgg
    12421 tggccttaga gtcttcctga atgccctgcg gatttctcgt gagcgacctg cagcccaagc
    12481 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12541 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12601 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12661 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
    12721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
    12781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    12841 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
    12901 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    12961 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
    13021 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
    13081 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    13141 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    13201 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    13261 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    13321 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    13381 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    13441 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    13501 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    13561 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    13621 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    13681 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    13741 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    13801 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    13861 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    13921 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    13981 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    14041 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    14101 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    14161 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    14221 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    14281 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    14341 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    14401 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    14461 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    14521 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    14581 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    14641 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    14701 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    14761 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    14821 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaat
//
