LOCUS       sequence-300133-       10602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NSP5 gene along with HA and BASU.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Meyers JM, Ramanathan M, Shanderson RL, Beck A, Donohue L, Ferguson 
            I, Guo MG, Rao DS, Miao W, Reynolds D, Yang X, Zhao Y, Yang YY, 
            Blish C, Wang Y, Khavari PA
  TITLE     The proximal proteome of 17 SARS-CoV-2 proteins links to disrupted 
            antiviral signaling and host translation.
  JOURNAL   PLoS Pathog. 2021 Oct 1;17(10):e1009412. doi: 
            10.1371/journal.ppat.1009412.
  PUBMED    34597346
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-10-1"; volume: "17(10):e1009412. doi"; pages: 
            " 10.1371/journal.ppat.1009412"
COMMENT     Sequence Label: SARS_COV2_nsp5_HA_BASU
FEATURES             Location/Qualifiers
     source          1..10602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4489..4512
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4516..4542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(5352..5926,5927..5929,5930..5938)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5519..5536)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5746..5765
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5958..6557
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5958..5977)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6454..6474
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6571..7159
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6624..6644)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7042..7053)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7366..7999
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8127..8143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8127..8143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8140..8162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8151..8167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8175..8181,8182..8199,8200..8205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8220..8241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8358..8375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8529..9117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8609..8628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9288..10079,10080..10148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9911..9930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10149..10253)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10301..10435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10338..10357)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10392..10411
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tgtctggttt taggaaaatg gcgttcccca gcggtaaagt tgaaggatgt
     3601 atggtccaag taacctgtgg taccactacc cttaatgggc tttggttgga cgacgtagtc
     3661 tactgccccc gacacgtaat ctgcactagt gaggatatgc ttaatcccaa ttacgaagac
     3721 cttttgattc ggaaatccaa tcacaacttc ctggtccaag cgggcaacgt ccaactcagg
     3781 gttattggac atagtatgca gaattgcgta ctgaagctca aagtcgatac tgcaaacccc
     3841 aagacgccca agtataaatt cgtccgaatc caaccaggcc aaacattttc cgtattggct
     3901 tgctataatg gaagccccag cggtgtctac caatgtgcaa tgagaccaaa ctttacgata
     3961 aagggttcat ttctgaacgg ctcttgcggt tccgttggtt ttaacatcga ctatgactgt
     4021 gtatcctttt gctacatgca ccatatggaa ctccctaccg gtgtccacgc cggtacagat
     4081 ctggaaggaa atttctacgg tccgttcgtt gaccggcaaa ccgcgcaagc ggctggaacc
     4141 gacacaacga ttacagtgaa tgtgctcgcg tggctgtacg cagcagtcat aaacggagac
     4201 aggtggtttc tgaaccgatt tacgacgact ctcaatgact tcaaccttgt tgcgatgaag
     4261 tacaattacg agccactcac ccaggaccat gttgatatcc tgggtcccct cagtgcccag
     4321 acagggatcg cagttctcga tatgtgcgcg tcactgaagg agcttctcca aaatggaatg
     4381 aatgggcgga ccatacttgg ttccgcactc ctcgaagatg aatttactcc atttgacgtg
     4441 gtcagacaat gcagtggggt cactttccag ctcgaaggcg gcgggggatg gagccatcca
     4501 caattcgaga aaggctaccc atacgatgtg cctgactatg ctggcaagtt gtctgaatca
     4561 gagattaggt ttggactgaa aacggaggtg atgggccaac atcttatcta tcacgatgtt
     4621 ttgtccagca cacaaaagac agcccatgaa ttggcaaata acaatgcccc cgagggtacc
     4681 ctcgtggttg cagataaaca gacagccggt aggggcggga tgagccgagt gtggcattct
     4741 caagaaggaa acggagtatg gatgagtttg atattgcgcc cggacattcc gctccaaaag
     4801 acgccccaac ttaccttgct ggcagctgta gcggtggttc agggaataga agaggccgcg
     4861 ggcatacaga ctgatataaa gtggccgaac gacatactca ttaacgggaa aaaaacagta
     4921 gggatactta ccgaaatgca agctgaggag gatcgagtgc gctccgtgat tattggtatt
     4981 ggcattaatg taaatcagca acccaatgat ttccccgatg agctcaagga cattgcgacg
     5041 agtctgtccc aggcagctgg ggaaaagatc gacagggcag gtgtaatcca gcatatcttg
     5101 ctttgtttcg aaaagcgata ccgagactac atgacccacg gtttcacgcc catcaagctc
     5161 ttgtgggagt catacgccct cggtattggc acgaacatga gggcgagaac tctcaacggg
     5221 acattttacg gtaaggcgtt gggtattgac gacgaagggg tacttttgtt ggaaactaat
     5281 gaaggcatta agaagatata cagcgcggac atcagcctta ggtgaccgca gtttgaaaag
     5341 taaggatccc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5401 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5461 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5521 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5581 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5641 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5701 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5761 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5821 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5881 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca
     5941 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     6001 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     6061 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     6121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     6181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     6481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     6541 gcaagcccgg tgcctagacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa
     6601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6721 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6781 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6841 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6901 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6961 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
     7021 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     7081 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     7141 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag
     7201 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag
     7261 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca
     7321 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc
     7381 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
     7441 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt
     7501 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca
     7561 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag
     7621 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt
     7681 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag
     7741 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag
     7801 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7981 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8041 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc
     8101 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10261 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta
    10321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10441 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10501 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10561 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
