LOCUS       sequence-30018-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  TITLE     Plasmids for Tagging Genes In-situ
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag2T-crelox-HYG
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..84)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgaggtcca tactaaccag gacccacttg actgagtcga cgataagctt ataacttcgt
       61 atagcataca ttatacgaag ttataccggt acacgttctc gtcggccggc cgctgcacgc
      121 gccttcgagt tttttttcct tttccccatt tttttcaact tgaagacttc aattacacca
      181 aaaaataaaa ttcacaaact tggtattcct ttgtgttaca ttcttgatcg ctcgcactga
      241 cattacagat cttactgttg gtaaatgatg aaaaagcctg aactcaccgc gacgtctgtc
      301 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
      361 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
      421 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
      481 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
      541 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
      601 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
      661 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatgg
//
