LOCUS       sequence-30020-e         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  TITLE     Plasmids for Tagging Genes In-situ
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag4H-crelox-HYG
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..88
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(106..139)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cctcgagtac ccttacgatg tgcctgatta cgcgtaccca tacgacgtgc cagactacgc
       61 atacccgtac gatgtgcccg attacgcata gtcgacgata agcttataac ttcgtatagc
      121 atacattata cgaagttata ccggtacacg ttctcgtcgg ccggccgctg cacgcgcctt
      181 cgagtttttt ttccttttcc ccattttttt caacttgaag acttcaatta caccaaaaaa
      241 taaaattcac aaacttggta ttcctttgtg ttacattctt gatcgctcgc actgacatta
      301 cagatcttac tgttggtaaa tgatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
      361 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
      421 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
      481 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
      541 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
      601 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
      661 gccggtcgc
//
