LOCUS       sequence-30022-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  TITLE     Plasmids for Tagging Genes In-situ
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag4HM-crelox-HYG
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             31..60
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             73..102
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             115..144
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             169..249
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     protein_bind    complement(267..300)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cccctcgagg gcggccgatc gagatccgag gagcagaagc tgatctcgga ggaggatctg
       61 ctgagatccg aggagcagaa gctgatctcg gaggaggatc tgctgagatc cgaggagcag
      121 aagctgatct cagaggagga tctgctgcga tctgaagcgg ccgtcgagta cccttacgat
      181 gtgcctgatt acgcgtaccc atacgacgtg ccagactacg catacccgta cgatgtgccc
      241 gattacgcat agtcgacgat aagcttataa cttcgtatag catacattat acgaagttat
      301 accggtacac gttctcgtcg gccggccgct gcacgcgcct tcgagttttt tttccttttc
      361 cccatttttt tcaacttgaa gacttcaatt acaccaaaaa ataaaattca caaacttggt
      421 attcctttgt gttacattct tgatcgctcg cactgacatt acagatctta ctgttggtaa
      481 atgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
      541 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      601 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      661 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga ngngcttgac
      721 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcac
//
