LOCUS       sequence-300231-       10935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10935)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133774
FEATURES             Location/Qualifiers
     source          1..10935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4402..4422
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4456..4533
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             4561..4581
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    5046..5167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5057..5076)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5111..5130
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5282..5328
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5358..5494
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5539..5610
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5836..6424)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5916..5935)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6924..8133
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7970..7989
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             8137..9165
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    9192..9240
                     /note="synthetic polyadenylation signal"
     CDS             join(9506..9574,9575..10366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9724..9743)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      10379..10515
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    10667..10738
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10743..10789)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10807..10855
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgctgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatca gatctttgtc
      541 gatcctacca tccactcgac acacccgcca gcggccgcca ccatgttcgt ctttcttgtg
      601 ttactccccc tggtttcgtc ccagtgcgtg aacttgacta cgcgaaccca actgccgcca
      661 gcatatacaa atagtttcac ccgtggagta tactaccctg ataaagtgtt tagatcaagc
      721 gtactacact ctactcagga cctgtttctc cccttcttct ccaatgtcac atggtttcat
      781 gcgatccacg ttagcggcac caacgggaca aagcgctttg acaaccctgt gctgccattc
      841 aatgatggtg tgtattttgc ttctaccgag aagagcaaca taattcgggg ctggatcttc
      901 ggaactaccc tcgacagtaa aactcagtca ttgctgattg tgaataacgc cacaaatgtc
      961 gttatcaagg tctgtgaatt tcaattctgc aacgatccat tccttggggt gtattaccat
     1021 aaaaataata agtcctggat ggagagcgaa tttagggtat actcgtctgc caacaattgt
     1081 actttcgagt atgtgtctca gcccttctta atggatctgg aaggtaaaca gggcaacttt
     1141 aagaacctcc gcgagtttgt attcaaaaat atagacggat actttaagat ttattccaag
     1201 cacacaccaa tcaatttggt gcgggatctc ccgcaagggt tcagcgctct ggaacctcta
     1261 gtggacctcc ccatcggcat taacatcaca agattccaga cgctgttagc cctgcataga
     1321 tcatacctta ctcccggtga cagtagttct gggtggaccg caggcgcggc tgcatattac
     1381 gtgggatatt tgcagcctcg gacgtttctg ctgaaataca acgagaacgg caccattaca
     1441 gatgccgtgg attgcgccct tgacccccta agcgaaacta agtgtacgct taaaagcttc
     1501 accgtcgaga agggaattta ccagacttcc aactttaggg tccagccgac agaatcaata
     1561 gttaggttcc caaatatcac caatttgtgc ccttttgggg aagtcttcaa tgctacacga
     1621 tttgcttcgg tttatgcctg gaacaggaag cgtatctcaa attgcgtcgc ggactacagc
     1681 gtgctctata atagtgcaag tttctcgacc tttaaatgtt atggcgtgtc cccgaccaaa
     1741 ctaaatgacc tctgttttac aaacgtttac gccgattctt ttgtgattcg cggtgatgag
     1801 gtacgccaaa tcgcaccggg ccagactggc aagattgccg attacaacta caagttgcct
     1861 gacgacttca ccggatgtgt tatagcctgg aatagcaata atctggattc taaggtcgga
     1921 ggtaactata actacctgta tagactgttc cgtaagtcaa acctgaaacc ctttgagcgg
     1981 gacatctcca cagagattta ccaagcaggg tctaccccct gcaatggggt tgaaggattc
     2041 aattgctact ttccattaca gagctacggg ttccagccta cgaacggggt tggttaccag
     2101 ccttataggg tagtggtgct gtcgttcgaa ctgttgcacg ctccagctac agtgtgcggg
     2161 cctaaaaaat caactaacct ggttaaaaat aagtgtgtta attttaactt caatggctta
     2221 accggaactg gagtgctcac ggagtcaaac aagaaatttc taccgtttca gcaattcggg
     2281 agggatatcg cagacactac ggacgcggtc agagatccac agacccttga gatccttgac
     2341 ataacaccct gctctttcgg aggtgtctcc gtaattacac caggcactaa tacctctaat
     2401 caggtggccg tgctttatca ggatgttaac tgtacagagg tcccggtggc tatccatgcc
     2461 gaccaattga cacctacgtg gcgcgtctat tctaccggct ccaacgtctt ccagacccgg
     2521 gctggatgct tgataggcgc agaacacgta aacaactctt acgaatgtga tattcccatt
     2581 ggagccggga tctgcgcaag ctaccagacc caaacaaaca gtcccagacg ggctcgtagc
     2641 gtggcctctc agagcataat agcctatacc atgtcccttg gcgcggagaa tagtgtggca
     2701 tactcaaata atagcatcgc tatccccact aactttacta tttccgtgac gactgagatt
     2761 ctgccagtgt ctatgactaa gacatccgtg gattgcacaa tgtatatttg tggggattcc
     2821 acagagtgta gcaacttgtt gctgcaatat ggatcattct gtacgcagct gaatcgcgcc
     2881 ctcaccggca tcgctgtgga acaggacaag aacactcaag aggtctttgc tcaggtgaag
     2941 caaatctaca agaccccacc tataaaagac ttcgggggtt ttaacttctc gcagattctc
     3001 cccgacccca gcaagccttc aaaaaggagc tttattgagg acctgttgtt caacaaggtt
     3061 actttagccg atgccggttt tatcaaacag tacggcgact gtcttggcga tatagcagcc
     3121 cgggatctga tttgcgcaca aaaattcaat ggccttaccg tactgccacc ccttctcacc
     3181 gacgaaatga tcgcgcagta cacctccgca ctgctggctg ggaccatcac ttcgggatgg
     3241 acctttgggg ctggtgctgc tttgcaaatt cccttcgcca tgcagatggc ctatcgattc
     3301 aatggcatag gggtaacaca gaacgtattg tatgaaaatc aaaagctaat cgctaaccag
     3361 tttaattcag ccattggtaa gatccaggat tcactctcca gtacagcctc tgccctggga
     3421 aagctccagg acgtggtcaa ccagaacgcg caggcattga atacactcgt caaacagctg
     3481 agtagcaatt tcggcgcaat cagtagtgtc ttaaacgata tactgagtag gcttgataaa
     3541 gtagaggccg aggtccagat tgacagacta atcacggggc gtcttcagag tttacagacc
     3601 tatgtgaccc aacagctgat ccgagcagcg gaaattcgag caagcgcgaa cctggccgct
     3661 actaaaatga gtgagtgcgt gctgggccaa agtaaacgag tcgacttttg cggcaaaggg
     3721 tatcatctca tgtctttccc tcagtccgcc cctcacggtg ttgttttcct ccacgtgaca
     3781 tacgttcccg cgcaggaaaa gaattttact acggcacccg ctatttgcca tgacggaaag
     3841 gctcattttc cacgggaggg agtgttcgtt tcaaacggga cacactggtt tgtaactcag
     3901 cggaactttt atgagcctca aatcatcacg acagacaata ccttcgtgag tggcaactgc
     3961 gatgtagtca taggcattgt gaataacact gtttatgatc cacttcagcc ggaactcgac
     4021 agcttcaaag aggaattgga taagtacttt aaaaatcata ccagtccaga cgtagatctc
     4081 ggcgatatat cgggcattaa cgccagtgtg gtcaatatac aaaaggagat tgacagactg
     4141 aatgaagtag ctaagaacct caacgaatct ctgattgacc tgcaagagct agggaagtac
     4201 gagcagtata tcaaatggcc atggtacata tggctgggtt ttatcgcggg tcttattgcc
     4261 atagtcatgg tgacgattat gttgtgctgt atgacctcct gctgttcttg tttgaaaggc
     4321 tgctgctcct gtggtagctg ttgcaaattc gatgaagatg actctgaacc cgtgttgaaa
     4381 ggcgttaagc tacactacac agaaaacctt tactttcagt cgggacggcg aatcccgggg
     4441 ctgataaatc cctggaaaag gagatggaag aagaacttta ttgcagtgtc tgcggctaac
     4501 cggttcaaaa agatcagttc aagcggcgcc ctagattacg acattcctac gaccgcctcc
     4561 gagaatctct atttccaggg tgaatttggc ttggcacaac atgacgaggc cgtcgataac
     4621 aagtttaata aagaacagca gaacgctttc tatgagatcc ttcacttacc aaatctgaac
     4681 gaagagcaac gcaacgcttt cattcagagc ctgaaagacg atccctcgca gtctgcaaat
     4741 ctcctggccg aagccaagaa gctgaatgac gcacaggctc caaaagttga taacaagttt
     4801 aacaaggagc agcagaatgc tttctacgag atattgcacc ttcctaatct gaatgaggaa
     4861 caaagaaatg catttatcca gtccctaaaa gacgatccat cacagagtgc caacctgctt
     4921 gcggaagcca aaaagctcaa tgatgcccag gccccgaaag tagacgcgaa ccatcaataa
     4981 ggatccgcag gcctctgcta gcttgactga ctgagataca gcgtaccttc agctcacaga
     5041 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     5101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     5161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     5221 ggttttttaa agcaagtaaa acctctacaa atgtggtatt ggcccatctc tatcggtatc
     5281 gtagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtg cccctcgggc
     5341 cggattgcta tctaccggca ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct
     5401 tatactccca catatgccag attcagcaac ggatacggct tccccaactt gcccacttcc
     5461 atacgtgtcc tccttaccag aaatttatcc ttaaggtcgt cagctatcct gcaggcgatc
     5521 tctcgatttc gatcaagaca ttcctttaat ggtcttttct ggacaccact aggggtcaga
     5581 agtagttcat caaactttct tccctcccta atctcattgg ttaccttggg ctatcgaaac
     5641 ttaattaacc agtcaagtca gctacttggc gagatcgact tgtctgggtt tcgactacgc
     5701 tcagaattgc gtcagtcaag ttcgatctgg tccttgctat tgcacccgtt ctccgattac
     5761 gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5821 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5881 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     5941 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6001 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6061 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6121 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6181 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6241 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6301 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6361 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     6421 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     6481 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     6541 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6601 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6661 atttaaattt ccgaactctc caaggccctc gtcggaaaat cttcaaacct ttcgtccgat
     6721 ccatcttgca ggctacctct cgaacgaact atcgcaagtc tcttggccgg ccttgcgcct
     6781 tggctattgc ttggcagcgc ctatcgccag gtattactcc aatcccgaat atccgagatc
     6841 gggatcaccc gagagaagtt caacctacat cctcaatccc gatctatccg agatccgagg
     6901 aatatcgaaa tcggggcgcg cctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc
     6961 ccccctcgtc acggcgagcg ctgccacgtc agacgaaggg cgcaggagcg tcctgatcct
     7021 tccgcccgga cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag
     7081 tatcagcaga aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt
     7141 ccagagagcg gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct
     7201 ccgtggggcg gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt
     7261 ccgtcgcagc cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg
     7321 gtgagtagcg ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga
     7381 cggaagcgtg tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct
     7441 gaactggggg ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag
     7501 acgcttgtga ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa
     7561 gaacccaagg tcttgagccc ttcgctaatg cgggaaagct cttattcggg tgagatgggc
     7621 tgggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc
     7681 gtctgttgcg ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg
     7741 agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg
     7801 ggccacctgc cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc
     7861 ctagggtagg ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg
     7921 aggcgtcagt ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg
     7981 ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt
     8041 gaaatgtaat catttgggtc aatatgtaat tttcagtgtt agacttgtaa attgtccgct
     8101 aaattctggc cgtttttggc ttttttgtta gacaacatgg gtaaaaagcc tgaactcacc
     8161 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     8221 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     8281 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     8341 gcatccgccg cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg
     8401 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     8461 ctgcccgctg ttctgcagcc ggtcgcggag gcaatggatg ccatcgctgc cgccgatctt
     8521 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     8581 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     8641 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     8701 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     8761 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     8821 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     8881 tacttcgagc ggaggcatcc ggagcttgca ggatcgcccc ggctccgggc gtatatgctc
     8941 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     9001 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     9061 caaatcgccc gcagaagcgc cgccgtctgg accgatggct gtgtagaagt actcgccgat
     9121 agtggaaacc gacgccccag cactcgtccg agggcaaagg aataagctag tatgtaagcc
     9181 tagtcttaga taataaaatc gctatccatc gaagatggat gtgtgttggt tttttgtgtg
     9241 tgtaacgcta ggcgcgcctg gtgtaccgag aacgatcctc tcagtgcgag tctcgacgat
     9301 ccatatcgtt gcttggcagt cagccagtcg gaatccagct tgggacccag gaagtccaat
     9361 cgtcagatat tgtactcaag cctggtcacg gcagcgtacc gatctgttta aacctagata
     9421 ttgatagtct gatcggtcaa cgtataatcg agtcctagct tttgcaaaca tctatcaaga
     9481 gacaggatca gcaggaggct ttcgcatgag tattcaacat ttccgtgtcg cccttattcc
     9541 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9601 agatgctgaa gatcagttgg gtgcgcgagt gggttacatc gaactggatc tcaacagcgg
     9661 taagatcctt gagagttttc gccccgaaga acgctttcca atgatgagca cttttaaagt
     9721 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     9781 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     9841 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9901 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     9961 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10021 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaaccttgc gtaaactatt
    10081 aactggcgaa ctacttactc tagcttcccg gcaacagttg atagactgga tggaggcgga
    10141 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    10201 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    10261 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    10321 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaaccga ttctaggtgc
    10381 attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc acatatgcca
    10441 gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc ctccttacca
    10501 gaaatttatc cttaagatcc cgaatcgttt aaactcgact ctggctctat cgaatctccg
    10561 tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc tcgtcgggca tcgaatctcg
    10621 tcagctatcg tcagcttacc tttttggcag cgatcgcggc tcccgacatc ttggaccatt
    10681 agctccacag gtatcttctt ccctctagtg gtcataacag cagcttcagc tacctctcaa
    10741 ttcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctat caatcgttgc
    10801 gttacacaca caaaaaacca acacacatcc atcttcgatg gatagcgatt ttattatcta
    10861 actgctgatc gagtgtagcc agatctagta atcaattacg gggtcattag ttcatagccc
    10921 atatatggag ttccg
//
