LOCUS       sequence-300245-        5493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5493)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134237
FEATURES             Location/Qualifiers
     source          1..5493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      242..378
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    530..601
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(606..652)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    670..718
                     /note="synthetic polyadenylation signal"
     enhancer        799..1102
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1104..1306
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1257..1277
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2638..2658
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2659..2685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2738..2859
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2749..2768)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2803..2822
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      2974..3020
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3050..3186
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3231..3302
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(3528..4116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3608..3627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(4862..4930,4931..5493,1..229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5080..5099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
       61 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      121 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
      181 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac cgattctagg
      241 tgcattggcg cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg
      301 ccagattcag caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta
      361 ccagaaattt atccttaaga tcccgaatcg tttaaactcg actctggctc tatcgaatct
      421 ccgtcgtttc gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc
      481 tcgtcagcta tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc
      541 attagctcca caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct
      601 caattcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt
      661 tgcgttacac acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat
      721 ctaactgctg atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag
      781 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      841 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      901 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      961 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1021 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1081 attagtcatc gctattacca tgctgatgcg gttttggcag tacatcaatg ggcgtggata
     1141 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1201 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     1261 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     1321 tcagatcaga tctttgtcga tcctaccatc cactcgacac acccgccagc ggccgccacc
     1381 atgtctgata atggacccca aaatcagcga aatgcacccc gcattacgtt tggtggaccc
     1441 tcagattcaa ctggcagtaa ccagaatgga gaacgcagtg gggcgcgatc aaaacaacgt
     1501 cggccccaag gtttacccaa taatactgcg tcttggttca ccgctctcac tcaacatggc
     1561 aaggaagacc ttaaattccc tcgaggacaa ggcgttccaa ttaacaccaa tagcagtcca
     1621 gatgaccaaa ttggctacta ccgaagagct accagacgaa ttcgtggtgg tgacggtaaa
     1681 atgaaagatc tcagtccaag atggtatttc tactacctag gaactgggcc agaagctgga
     1741 cttccctatg gtgctaacaa agacggcatc atatgggttg caactgaggg agccttgaat
     1801 acaccaaaag atcacattgg cacccgcaat cctgctaaca atgctgcaat cgtgctacaa
     1861 cttcctcaag gaacaacatt gccaaaaggc ttctacgcag aagggagcag aggcggcagt
     1921 caagcctctt ctcgttcctc atcacgtagt cgcaacagtt caagaaattc aactccaggc
     1981 agcagtaggg gaacttctcc tgctagaatg gctggcaatg gcggtgatgc tgctcttgct
     2041 ttgctgctgc ttgacagatt gaaccagctt gagagcaaaa tgtctggtaa aggccaacaa
     2101 caacaaggcc aaactgtcac taagaaatct gctgctgagg cttctaagaa gcctcggcaa
     2161 aaacgtactg ccactaaagc atacaatgta acacaagctt tcggcagacg tggtccagaa
     2221 caaacccaag gaaattttgg ggaccaggaa ctaatcagac aaggaactga ttacaaacat
     2281 tggccgcaaa ttgcacaatt tgcccccagc gcttcagcgt tcttcggaat gtcgcgcatt
     2341 ggcatggaag tcacaccttc gggaacgtgg ttgacctaca caggtgccat caaattggat
     2401 gacaaagatc caaatttcaa agatcaagtc attttgctga ataagcatat tgacgcatac
     2461 aaaacattcc caccaacaga gcctaaaaag gacaaaaaga agaaggctga tgaaactcaa
     2521 gccttaccgc agagacagaa gaaacagcaa actgtgactc ttcttcctgc tgcagatttg
     2581 gatgatttct ccaaacaatt gcaacaatcc atgagcagtg ctgactcaac tcaggccgaa
     2641 aacctttact ttcagtcgta cccctatgac gtaccagatt atgcgtaagc tagcttgact
     2701 gactgagata cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga
     2761 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2821 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2881 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     2941 aaatgtggta ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac
     3001 gggtcttgag gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca
     3061 gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca
     3121 acggatacgg cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat
     3181 ccttaaggtc gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta
     3241 atggtctttt ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc
     3301 taatctcatt ggttaccttg ggctatcgaa acttaattaa ccagtcaagt cagctacttg
     3361 gcgagatcga cttgtctggg tttcgactac gctcagaatt gcgtcagtca agttcgatct
     3421 ggtccttgct attgcacccg ttctccgatt acgagtttca tttaaatcat gtgagcaaaa
     3481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4321 gcgatctgtc tatttcgttc atccatagtt gcatttaaat ttccgaactc tccaaggccc
     4381 tcgtcggaaa atcttcaaac ctttcgtccg atccatcttg caggctacct ctcgaacgaa
     4441 ctatcgcaag tctcttggcc ggccttgcgc cttggctatt gcttggcagc gcctatcgcc
     4501 aggtattact ccaatcccga atatccgaga tcgggatcac ccgagagaag ttcaacctac
     4561 atcctcaatc ccgatctatc cgagatccga ggaatatcga aatcggggcg cgcctggtgt
     4621 accgagaacg atcctctcag tgcgagtctc gacgatccat atcgttgctt ggcagtcagc
     4681 cagtcggaat ccagcttggg acccaggaag tccaatcgtc agatattgta ctcaagcctg
     4741 gtcacggcag cgtaccgatc tgtttaaacc tagatattga tagtctgatc ggtcaacgta
     4801 taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag gaggctttcg
     4861 catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4921 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4981 gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5041 cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5101 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5161 ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5221 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5281 tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5341 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5401 gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac ttactctagc
     5461 ttcccggcaa cagttgatag actggatgga ggc
//
