LOCUS       sequence-300325-         921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
ORIGIN
        1 atgtccggat tccgcaagat ggccttccct tctggcaagg tggagggctg catggtgcag
       61 gtgacctgcg gaaccaccac cctgaacggt ctgtggctgg acgacgtggt gtactgcccc
      121 aggcacgtga tctgcacctc tgaggacatg ctgaacccta actacgaaga cctgctgatc
      181 agaaagagca accacaactt cctggtgcag gccggtaacg tgcagctgcg tgtgatcggc
      241 cactctatgc agaactgcgt gctgaagctg aaggtggaca ccgctaaccc caagacccct
      301 aagtacaagt tcgtgagaat ccagcctggt cagaccttct ccgtgctggc ctgctacaac
      361 ggaagcccat ccggcgtgta ccagtgcgct atgcgtccca acttcaccat caagggcagc
      421 ttcctgaacg gctctgccgg aagcgtgggt ttcaacatcg actacgactg cgtgtccttc
      481 tgctacatgc accacatgga gctgccaact ggagtgcacg ctggaactga cctggagggt
      541 aacttctacg gccccttcgt ggacagacag actgctcagg ctgctggcac cgacaccacc
      601 atcaccgtga acgtgctggc ttggctgtac gccgctgtga tcaacggaga ccgctggttc
      661 ctgaacaggt tcaccaccac cctgaacgac ttcaacctgg tggctatgaa gtacaactac
      721 gagcctctga cccaggacca cgtggatatc ctgggtccac tgagcgccca gactggaatc
      781 gctgtgctgg acatgtgcgc ctccctgaag gaactgctgc agaacggcat gaacggacgc
      841 accatcctgg gatccgctct gctggaggac gaattcaccc ccttcgacgt ggtgaggcag
      901 tgctctggtg tgaccttcca g
//
