LOCUS       sequence-300340-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified Tobacco Rattle Virus RNA 2; used for virus induced gene 
            silencing in plants along with pYL192 (TRV RNA1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Liu Y, Schiff M, Marathe R, Dinesh-Kumar SP
  TITLE     Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for 
            N-mediated resistance to tobacco mosaic virus.
  JOURNAL   Plant J. 2002 May;30(4):415-29. doi: 
            10.1046/j.1365-313x.2002.01297.x.
  PUBMED    12028572
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1046/j.1365-313x.2002.01297"; journalName: "Plant J"; date: 
            "2002-05"; volume: "30"; issue: "4"; pages: "415-29"
COMMENT     Sequence Label: pYL156 (TRV RNA2)
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        427..772
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     715..735
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2933..2950
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2985..3237
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3259..3283
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4584..5213
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5642..6715
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6781..6975
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7211..7233)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7319..7459
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7374..7393
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7474..7491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7645..8233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7725..7744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9658..10452)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10877..10901
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
       61 atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca aagggtaata
      121 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta
      181 gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
      241 gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg tcaacatggt
      361 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag
      421 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
      481 agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca
      541 tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga
      601 tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      661 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
      721 ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga taaaacattg
      781 cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg tactaattga
      841 caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc tctccctgac
      901 gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc gtacgggaaa
      961 gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta ggttagtaag
     1021 aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg aaagtagcaa
     1081 tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt tttaattaac
     1141 ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt agagaaaact
     1201 agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt gtctgctgat
     1261 aagatcattt taatttggac gattagactt gttgaactac aggttactga atcacttgcg
     1321 ctaatcaaca tgggagatat gtacgatgaa tcatttgaca agtcgggcgg tcctgctgac
     1381 ttgatggacg attcttgggt ggaatcagtt tcgtggaaag atctgttgaa gaagttacac
     1441 agcataaaat ttgcactaca gtctggtaga gatgagatca ctgggttact agcggcactg
     1501 aatagacagt gtccttattc accatatgag cagtttccag ataagaaggt gtatttcctt
     1561 ttagactcac gggctaacag tgctcttggt gtgattcaga acgcttcagc gttcaagaga
     1621 cgagctgatg agaagaatgc agtggcgggt gttacaaata ttcctgcgaa tccaaacaca
     1681 acggttacga cgaaccaagg gagtactact actaccaagg cgaacactgg ctcgactttg
     1741 gaagaagact tgtacactta ttacaaattc gatgatgcct ctacagcttt ccacaaatct
     1801 ctaacttcgt tagagaacat ggagttgaag agttattacc gaaggaactt tgagaaagta
     1861 ttcgggatta agtttggtgg agcagctgct agttcatctg caccgcctcc agcgagtgga
     1921 ggtccgatac gtcctaatcc ctagggattt aaggacgtga actctgttga gatctctgtg
     1981 aaattcagag ggtgggtgat accatattca ctgatgccat tagcgacatc taaatagggc
     2041 taattgtgac taatttgagg gaatttcctt taccattgac gtcagtgtcg ttggtagcat
     2101 ttgagtttcg caatgcacga attacttagg aagtggcttg acgacactaa tgtgttattg
     2161 ttagataatg gtttggtggt caaggtacgt agtagagtcc cacatattcg cacgtatgaa
     2221 gtaattggaa agttgtcagt ttttgataat tcactgggag atgatacgct gtttgaggga
     2281 aaagtagaga acgtatttgt ttttatgttc aggcggttct tgtgtgtcaa caaagatgga
     2341 cattgttact caaggaagca cgatgagctt tattattacg gacgagtgga cttagattct
     2401 gtgagtaagg ttaccgaatt ctctagaagg cctccatggg gatccggtac cgagctcacg
     2461 cgtctcgagg cccgggcatg tcccgaagac attaaactac ggttctttaa gtagatccgt
     2521 gtctgaagtt ttaggttcaa tttaaaccta cgagattgac attctcgact gatcttgatt
     2581 gatcggtaag tcttttgtaa tttaattttc tttttgattt tattttaaat tgttatctgt
     2641 ttctgtgtat agactgtttg agatcggcgt ttggccgact cattgtctta ccatagggga
     2701 acggactttg tttgtgttgt tattttattt gtattttatt aaaattctca acgatctgaa
     2761 aaagcctcgc ggctaagaga ttgttggggg gtgagtaagt acttttaaag tgatgatggt
     2821 tacaaaggca aaaggggtaa aacccctcgc ctacgtaagc gttattacgc ccgtctgtac
     2881 ttatatcagt acactgacga gtccctaaag gacgaaacgg gagaacgcta gccaccacca
     2941 ccaccaccac gtgtgaatta caggtgacca gctcgaattt ccccgatcgt tcaaacattt
     3001 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     3061 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     3121 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     3181 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     3241 aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     3301 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     3361 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     3421 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     3481 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     3541 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     3601 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     3661 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     3721 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     3781 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     3841 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     3901 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     3961 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4021 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4081 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4141 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4201 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4261 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     4321 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     4381 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     4441 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     4501 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     4561 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     4621 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     4681 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     4741 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     4801 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     4861 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     4921 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     4981 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5041 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5101 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5161 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5221 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     5281 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     5341 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     5401 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     5461 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     5521 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     5581 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     5641 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     5701 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     5761 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     5821 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     5881 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     5941 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     6001 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     6061 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     6121 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     6181 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     6241 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     6301 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     6361 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     6421 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     6481 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     6541 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     6601 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     6661 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     6721 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     6781 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     6841 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     6901 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     6961 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     7021 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     7081 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     7141 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     7201 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     7261 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     7321 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     7381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7441 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7501 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7561 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7621 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7681 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7741 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7801 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7861 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7921 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7981 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8041 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8101 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8161 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8221 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     8281 aaactcacgt taagggctga tgaatcccct aatgattttt atcaaaatca ttaagttaag
     8341 gtagatacac atcttgtcat atgatcaaat ggtttcgcca aaaatcaata atcagacaac
     8401 aaaatgtgcg aactcgatat tttacacgac tctctttacc aattctgccc cgaattacac
     8461 ttaaaacgac tcaacagctt aacgttggct tgccacgcct tacttgactg taaaactctc
     8521 actcttaccg aacttggccg taacctgcca accaaagcga gaacaaaaca taacatcaaa
     8581 cgaatcgacc gattgttagg taatcgtcac ctccacaaag agcgactcgc tgtataccgt
     8641 tggcatgcta gctttatctg ttcgggcaat acgatgccca ttgtacttgt tgactggtct
     8701 gatatccgtg agcaaaaacg gcttatggta ttgcgagctt cagtcgcact acacggtcgt
     8761 tctgttactc tttatgagaa agcgttcccg ctttcagagc aatgttcaaa gaaagctcat
     8821 gaccaatttc tagccgacct tgcgagcatt ctaccgagta acaccacacc gctcattgtc
     8881 agtgatgctg gctttaaagt gccatggtat aaatccgttg agaagctggg ttggtactgg
     8941 ttaagtcgag taagaggaaa agtacaatat gcagacctag gagcggaaaa ctggaaacct
     9001 atcagcaact tacatgatat gtcatctagt cactcaaaga ctttaggcta taagaggctg
     9061 actaaaagca atccaatctc atgccaaatt ctattgtata aatctcgctc taaaggccga
     9121 aaaaatcagc gctcgacacg gactcattgt caccacccgt cacctaaaat ctactcagcg
     9181 tcggcaaagg agccatggat tctagcaact aacttacctg ttgaaattcg aacacccaaa
     9241 caacttgtta atatctattc gaagcgaatg cagattgaag aaaccttccg agacttgaaa
     9301 agtcctgcct acggactagg cctacgccat agccgaacga gcagctcaga gcgttttgat
     9361 atcatgctgc taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg cgttcatgct
     9421 cagaaacaag gttgggacaa gcacttccag gctaacacag tcagaaatcg aaacgtactc
     9481 tcaacagttc gcttaggcat ggaagttttg cggcattctg gctacacaat aacaagggaa
     9541 gactcactcg tggctgcaac cctgcttact caaaatctat tcacacatgg ttacgttttg
     9601 gggaaattat gaggggatct ctcagcgtta agggattttg gtcatgcatt ctaggtacta
     9661 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
     9721 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
     9781 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
     9841 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
     9901 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
     9961 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    10021 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    10081 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    10141 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    10201 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    10261 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    10321 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    10381 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    10441 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    10501 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    10561 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    10621 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    10681 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    10741 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    10801 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    10861 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
    10921 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcctaggc
    10981 caccatgttg ggcccggcgc gcc
//
