LOCUS       sequence-300342-        2340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 4E1H.95_LUMABS sensor in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Yang C, Sesterhenn F, Bonet J, van Aalen EA, Scheller L, Abriata LA,
            Cramer JT, Wen X, Rosset S, Georgeon S, Jardetzky T, Krey T, 
            Fussenegger M, Merkx M, Correia BE
  TITLE     Bottom-up de novo design of functional proteins with complex 
            structural features.
  JOURNAL   Nat Chem Biol. 2021 Apr;17(4):492-500. doi: 
            10.1038/s41589-020-00699-x. Epub 2021 Jan 4.
  PUBMED    33398169
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-020-00699-x"; journalName: "Nat Chem Biol"; date: 
            "2021-04"; volume: "17"; issue: "4"; pages: "492-500"
COMMENT     Sequence Label: 4E1H.95_LUMABS
FEATURES             Location/Qualifiers
     source          1..2340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             178..189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagcg acgacaactt tatatacaag gcgaaggcgc tctatcccta tgatgcagat
      121 gatgatgacg catacgaaat ctcattcgaa caaaatgaaa tccttcaggt atcagacatt
      181 gaggggcggt ggtggaaagc ccgccgcgcc aatggagaga caggcatcat cccgtcgaat
      241 tatgttcagt tgattgacgg tcctgaggaa atgcatcggg gcggctcggg aggctcaggc
      301 agccatatgg taagtaaagg tgaagaagac aatatggctt ctctgcctgc cacacatgag
      361 cttcatattt ttgggagcat aaacggagtg gatttcgaca tggtaggtca gggtacgggg
      421 aaccctaacg atggatatga ggagttgaat cttaaaagca caaagggtga tctgcagttc
      481 tcgccctgga tcctggtgcc gcatataggt tatggtttcc atcagtatct tccatacccg
      541 gatggcatga gcccttttca ggccgcaatg gtagatggct caggatatca agtgcatcgg
      601 accatgcagt ttgaagatgg ggcgtctttg acggtaaatt acaggtacac ctatgagggt
      661 agccatataa agggagaagc gcaggtgaag ggaactggat tcccagcgga tggcccagtc
      721 atgacaaaca gcctcaccgc tgctgattgg tgccgatcca agaaaacgta tccaaacgat
      781 aaaactatca tttctacttt taagtggtcc tatacaacag gaaacgggaa acgctatcgt
      841 tcaacggccc gcacgaccta cacgtttgca aagccaatgg ctgcgaatta tctgaaaaac
      901 cagccgatgt atgtgttccg taaaaccgaa ctgaaacatt ctaaaacgga gctcaatttc
      961 aaggaatggc agaaggcatt tacggatgtc atgggaatgg acgaactgta taagtcgggc
     1021 ggaggtacca agtattttga ctgtaccgtc tccggcgagc gcggcattat caagacgtat
     1081 gggatacaac ttcctgaaga ggcccttaag gagcacgttc gcgagtacgt cgaaaaactc
     1141 agggaaggct ctgctataac aatcacttgt acggcagggg atcgagtatt caagtttaag
     1201 gataaggtag gtgggggttc tggcggttct ggcggctcgg gaggttctgg gggttcagga
     1261 ggtagtggtg cagaagcagc cgctaaagaa gccgcggcta aagaagccgc agcgaaagaa
     1321 gctgcggcaa aagaggccgc agcaaaagag gctgcggcga aggccggatc cggtggttcc
     1381 gggggcagcg gagggagtgg cggaagcggg ggttctggtg ctgaggcggc ggcgaaggaa
     1441 gctgccgcta aggaagcggc agcaaaagag gcggcagcca aagaggcggc cgccaaggag
     1501 gcagcggcta aagcgggaag tggaggttcc ggtggttcag gtggaagtgg cggatctggt
     1561 ggctctggcg ggaagtattt tgactgcacg gtgtctggtg agcgcggaat catcaaaact
     1621 tacggcatcc agcttcccga ggaggctctg aaagagcacg ttcgcgaata cgtggagaaa
     1681 ctgcgtgaag gatcggcaat taccatcacc tgcactgctg gtgatcgtgt tttcaagttc
     1741 aaagataagg ttggtactag tggaggcagc atggtattta ctcttgaaga ttttgtcggt
     1801 gattggcgcc agaccgccgg ctataacctg gaccaagtgc ttgaacaggg cggggttagc
     1861 agcctgtttc aaaacctggg ggtgagtgtc acgccaattc agcgcatcgt tctgtcggga
     1921 gagaatggtc tgaaaatcga tatccacgtc attatcccgt acgaaggtct ttctggtgat
     1981 cagatggggc agatagaaaa aatattcaaa gtggtgtacc cagtagacga tcatcacttc
     2041 aaggttatac tgcactatgg caccctcgtt atcgatggcg ttactccgaa tatgatcgat
     2101 tactttgggc gtccttatga aggtattgcg gtgttcgacg gtaaaaaaat tacggttacc
     2161 gggacgctct ggaatggtaa taaaatcatt gatgagcgct tgataaaccc agatggcagc
     2221 cttctgttca gagttacgat aaacggggtt acgggttggc gactgtgcga aagaatatta
     2281 gcttctagcg gtggaggatc tattcgttca aagccgctgc cacccctacc cgtgtaatag
//
