LOCUS       sequence-300343-        2490 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 4E2H.210_LUMABS sensor in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Yang C, Sesterhenn F, Bonet J, van Aalen EA, Scheller L, Abriata LA,
            Cramer JT, Wen X, Rosset S, Georgeon S, Jardetzky T, Krey T, 
            Fussenegger M, Merkx M, Correia BE
  TITLE     Bottom-up de novo design of functional proteins with complex 
            structural features.
  JOURNAL   Nat Chem Biol. 2021 Apr;17(4):492-500. doi: 
            10.1038/s41589-020-00699-x. Epub 2021 Jan 4.
  PUBMED    33398169
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-020-00699-x"; journalName: "Nat Chem Biol"; date: 
            "2021-04"; volume: "17"; issue: "4"; pages: "492-500"
COMMENT     Sequence Label: 4E2H.210_LUMABS
FEATURES             Location/Qualifiers
     source          1..2490
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             178..189
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atggctagcg acgacaactt tatatacaag gcgaaggcgc tctatcccta tgatgcagat
      121 gatgatgacg catacgaaat ctcattcgaa caaaatgaaa tccttcaggt atcagacatt
      181 gaggggcggt ggtggaaagc ccgccgcgcc aatggagaga caggcatcat cccgtcgaat
      241 tatgttcagt tgattgacgg tcctgaggaa atgcatcggg gcggctcggg aggctcaggc
      301 agccatatgg taagtaaagg tgaagaagac aatatggctt ctctgcctgc cacacatgag
      361 cttcatattt ttgggagcat aaacggagtg gatttcgaca tggtaggtca gggtacgggg
      421 aaccctaacg atggatatga ggagttgaat cttaaaagca caaagggtga tctgcagttc
      481 tcgccctgga tcctggtgcc gcatataggt tatggtttcc atcagtatct tccatacccg
      541 gatggcatga gcccttttca ggccgcaatg gtagatggct caggatatca agtgcatcgg
      601 accatgcagt ttgaagatgg ggcgtctttg acggtaaatt acaggtacac ctatgagggt
      661 agccatataa agggagaagc gcaggtgaag ggaactggat tcccagcgga tggcccagtc
      721 atgacaaaca gcctcaccgc tgctgattgg tgccgatcca agaaaacgta tccaaacgat
      781 aaaactatca tttctacttt taagtggtcc tatacaacag gaaacgggaa acgctatcgt
      841 tcaacggccc gcacgaccta cacgtttgca aagccaatgg ctgcgaatta tctgaaaaac
      901 cagccgatgt atgtgttccg taaaaccgaa ctgaaacatt ctaaaacgga gctcaatttc
      961 aaggaatggc agaaggcatt tacggatgtc atgggaatgg acgaactgta taagtcgggc
     1021 ggaggtaccc cgaacaccat ctcatgcacc gcccctaccc gtgatgagct tgctcagtgc
     1081 gtccaagaac attggaagga gtgggcgaag aaacttgacg gtagcaaagt gacgttctct
     1141 gcgaacgtcc cgaaattaag tcggggcatc attaaaactt cagatggcaa tatcgaagag
     1201 gtgctgcaag ctctggctaa agcactgaag gagggagtgt cggttgaagg cgggacagtc
     1261 acaataagcc tgaagggaag cacgcgtggg ggttcgggtg gttcaggcgg ctcaggaggc
     1321 tccgggggtt ccggagggag cggtgctgaa gccgcagcca aggaagcagc agctaaagag
     1381 gccgctgcga aggaagctgc cgcaaaggag gcggcggcga aagaggcggc agcaaaagcc
     1441 ggatctggtg gcagtggtgg ctccggcggg tcaggtggca gcgggggatc aggagctgag
     1501 gcagccgcca aagaggctgc ggccaaggag gccgccgcta aagaagccgc ggcaaaagag
     1561 gcagcggcaa aggaagcggc tgcgaaagcc ggaagtggtg ggtcgggcgg ctccggtggc
     1621 tctggcggca gtggcggtag tggcggggtc gacccgaata ctatctcatg cactgcacca
     1681 acgcgcgatg aattagccca atgcgtccaa gagcactgga aagaatgggc aaagaaactg
     1741 gacgggtcaa aagtcacctt ttcggccaac gtgccgaaac tttcgcgtgg aataattaag
     1801 acgtcggatg ggaatatcga agaagtgctg caggcccttg caaaggctct gaaggagggc
     1861 gttagtgttg agggcggaac agttaccatt tccttaaaag gcagcactag tggaggcagc
     1921 atggtattta ctcttgaaga ttttgtcggt gattggcgcc agaccgccgg ctataacctg
     1981 gaccaagtgc ttgaacaggg cggggttagc agcctgtttc aaaacctggg ggtgagtgtc
     2041 acgccaattc agcgcatcgt tctgtcggga gagaatggtc tgaaaatcga tatccacgtc
     2101 attatcccgt acgaaggtct ttctggtgat cagatggggc agatagaaaa aatattcaaa
     2161 gtggtgtacc cagtagacga tcatcacttc aaggttatac tgcactatgg caccctcgtt
     2221 atcgatggcg ttactccgaa tatgatcgat tactttgggc gtccttatga aggtattgcg
     2281 gtgttcgacg gtaaaaaaat tacggttacc gggacgctct ggaatggtaa taaaatcatt
     2341 gatgagcgct tgataaaccc agatggcagc cttctgttca gagttacgat aaacggggtt
     2401 acgggttggc gactgtgcga aagaatatta gcttctagcg gtggaggatc tattcgttca
     2461 aagccgctgc cacccctacc cgtgtaatag
//
