LOCUS       sequence-300349-       10398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10398)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4133930
FEATURES             Location/Qualifiers
     source          1..10398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4098..4118
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4119..4136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4205..4326
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4216..4235)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4270..4289
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4441..4487
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4517..4653
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4698..4769
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(4995..5583)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5075..5094)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6083..7292
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7129..7148
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             7296..8324
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    8351..8399
                     /note="synthetic polyadenylation signal"
     CDS             join(8665..8733,8734..9525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8883..8902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      9538..9674
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9826..9897
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9902..9948)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9966..10014
                     /note="synthetic polyadenylation signal"
     enhancer        10095..10398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatcagatc tttgtcgatc
      241 ctaccatcca ctcgacacac ccgccagcgg ccgccaccat gtttgttttt cttgttttat
      301 tgccactagt ctctagtcag tgtgttaatc ttacaaccag aactcaatta ccccctgcat
      361 acactaattc tttcacacgt ggtgtttatt accctgacaa agttttcaga tcctcagttt
      421 tacattcaac tcaggacttg ttcttacctt tcttttccaa tgttacttgg ttccatgcta
      481 tacatgtctc tgggaccaat ggtactaaga ggtttgataa ccctgtccta ccatttaatg
      541 atggtgttta ttttgcttcc actgagaagt ctaacataat aagaggctgg atttttggta
      601 ctactttaga ttcgaagacc cagtccctac ttattgttaa taacgctact aatgttgtta
      661 ttaaagtctg tgaatttcaa ttttgtaatg atccattttt gggtgtttat taccacaaaa
      721 acaacaaaag ttggatggaa agtgagttca gagtttattc tagtgcgaat aattgcactt
      781 ttgaatatgt ctctcagcct tttcttatgg accttgaagg aaaacagggt aatttcaaaa
      841 atcttaggga atttgtgttt aagaatattg atggttattt taaaatatat tctaagcaca
      901 cgcctattaa tttagtgcgt gatctccctc agggtttttc ggctttagaa ccattggtag
      961 atttgccaat aggtattaac atcactaggt ttcaaacttt acttgcttta catagaagtt
     1021 atttgactcc tggtgattct tcttcaggtt ggacagctgg tgctgcagct tattatgtgg
     1081 gttatcttca acctaggact tttctattaa aatataatga aaatggaacc attacagatg
     1141 ctgtagactg tgcacttgac cctctctcag aaacaaagtg tacgttgaaa tccttcactg
     1201 tagaaaaagg aatctatcaa acttctaact ttagagtcca accaacagaa tctattgtta
     1261 gatttcctaa tattacaaac ttgtgccctt ttggtgaagt ttttaacgcc accagatttg
     1321 catctgttta tgcttggaac aggaagagaa tcagcaactg tgttgctgat tattctgtcc
     1381 tatataattc cgcatcattt tccactttta agtgttatgg agtgtctcct actaaattaa
     1441 atgatctctg ctttactaat gtctatgcag attcatttgt aattagaggt gatgaagtca
     1501 gacaaatcgc tccagggcaa actggaaaga ttgctgatta taattataaa ttaccagatg
     1561 attttacagg ctgcgttata gcttggaatt ctaacaatct tgattctaag gttggtggta
     1621 attataatta cctgtataga ttgtttagga agtctaatct caaacctttt gagagagata
     1681 tttcaactga aatctatcag gccggtagca caccttgtaa tggtgttgaa ggttttaatt
     1741 gttactttcc tttacaatca tatggtttcc aacccactaa tggtgttggt taccaaccat
     1801 acagagtagt agtactttct tttgaacttc tacatgcacc agcaactgtt tgtggaccta
     1861 aaaagtctac taatttggtt aaaaacaaat gtgtcaattt caacttcaat ggtttaacag
     1921 gcacaggtgt tcttactgag tctaacaaaa agtttctgcc tttccaacaa tttggcagag
     1981 acattgctga cactactgat gctgtccgtg atccacagac acttgagatt cttgacatta
     2041 caccatgttc ttttggtggt gtcagtgtta taacaccagg aacaaatact tctaaccagg
     2101 ttgctgttct ttatcaggat gttaactgca cagaagtccc tgttgctatt catgcagatc
     2161 aacttactcc tacttggcgt gtttattcta caggttctaa tgtttttcaa acacgtgcag
     2221 gctgtttaat aggggctgaa catgtcaaca actcatatga gtgtgacata cccattggtg
     2281 caggtatatg cgctagttat cagactcaga ctaattctcc tcggcgggca cgtagtgtag
     2341 ctagtcaatc catcattgcc tacactatgt cacttggtgc agaaaattca gttgcttact
     2401 ctaataactc tattgccata cccacaaatt ttactattag tgttaccaca gaaattctac
     2461 cagtgtctat gaccaagaca tcagtagatt gtacaatgta catttgtggt gattcaactg
     2521 aatgcagcaa tcttttgttg caatatggca gtttttgtac acaattaaac cgtgctttaa
     2581 ctggaatagc tgttgaacaa gacaaaaaca cccaagaagt ttttgcacaa gtcaaacaaa
     2641 tttacaaaac accaccaatt aaagattttg gtggttttaa tttttcacaa atattaccag
     2701 atccatcaaa accaagcaag aggtcattta ttgaagatct acttttcaac aaagtgacac
     2761 ttgcagatgc tggcttcatc aaacaatatg gtgattgcct tggtgatatt gctgctagag
     2821 acctcatttg tgcacaaaag tttaacggcc ttactgtttt gccacctttg ctcacagatg
     2881 aaatgattgc tcaatacact tctgcactgt tagcgggtac aatcacttct ggttggacct
     2941 ttggtgcagg tgctgcatta caaataccat ttgctatgca aatggcttat aggtttaatg
     3001 gtattggagt tacacagaat gttctctatg agaaccaaaa attgattgcc aaccaattta
     3061 atagtgctat tggcaaaatt caagactcac tttcttccac agcaagtgca cttggaaaac
     3121 ttcaagatgt ggtcaaccaa aatgcacaag ctttaaacac gcttgttaaa caacttagct
     3181 ccaattttgg tgcaatttca agtgttttaa atgatatcct ttcacgtctt gacaaagttg
     3241 aggctgaagt gcaaattgat aggttgatca caggcagact tcaaagtttg cagacatatg
     3301 tgactcaaca attaattaga gctgcagaaa tcagagcttc tgctaatctt gctgctacta
     3361 aaatgtcaga gtgtgtactt ggacaatcaa aaagagttga tttttgtgga aagggctatc
     3421 atcttatgtc cttccctcag tcagcacctc atggtgtagt cttcttgcat gtgacttatg
     3481 tccctgcaca agaaaagaac ttcacaactg ctcctgccat ttgtcatgat ggaaaagcac
     3541 actttcctcg tgaaggtgtc tttgtttcaa atggcacaca ctggtttgta acacaaagga
     3601 atttttatga accacaaatc attactacag acaacacatt tgtgtctggt aactgtgatg
     3661 ttgtaatagg aattgtcaac aacacagttt atgatccttt gcaacctgaa ttagactcat
     3721 tcaaggagga gttagataaa tattttaaga atcatacatc accagatgtt gatttaggtg
     3781 acatctctgg cattaatgct tcagttgtaa acattcaaaa agaaattgac cgcctcaatg
     3841 aggttgccaa gaatttaaat gaatctctca tcgatctcca agaacttgga aagtatgagc
     3901 agtatataaa atggccatgg tacatttggc taggttttat agctggcttg attgccatag
     3961 taatggtgac aattatgctt tgctgtatga ccagttgctg tagttgtctc aagggctgtt
     4021 gttcttgtgg atcctgctgc aaatttgatg aagacgactc tgagccagtg ctcaaaggag
     4081 tcaaattaca ttacacagaa aacctttact ttcagtcgca ccatcaccat caccattaag
     4141 gatccgcagg cctctgctag cttgactgac tgagatacag cgtaccttca gctcacagac
     4201 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     4261 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4321 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4381 gttttttaaa gcaagtaaaa cctctacaaa tgtggtattg gcccatctct atcggtatcg
     4441 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgtgc ccctcgggcc
     4501 ggattgctat ctaccggcat tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt
     4561 atactcccac atatgccaga ttcagcaacg gatacggctt ccccaacttg cccacttcca
     4621 tacgtgtcct ccttaccaga aatttatcct taaggtcgtc agctatcctg caggcgatct
     4681 ctcgatttcg atcaagacat tcctttaatg gtcttttctg gacaccacta ggggtcagaa
     4741 gtagttcatc aaactttctt ccctccctaa tctcattggt taccttgggc tatcgaaact
     4801 taattaacca gtcaagtcag ctacttggcg agatcgactt gtctgggttt cgactacgct
     4861 cagaattgcg tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg
     4921 agtttcattt aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4981 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     5041 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     5101 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     5161 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     5221 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     5281 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5341 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5401 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5461 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5521 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5581 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5641 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5701 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5761 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca
     5821 tttaaatttc cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc
     5881 catcttgcag gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt
     5941 ggctattgct tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg
     6001 ggatcacccg agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga
     6061 atatcgaaat cggggcgcgc ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc
     6121 cccctcgtca cggcgagcgc tgccacgtca gacgaagggc gcaggagcgt cctgatcctt
     6181 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt
     6241 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc
     6301 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc
     6361 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc
     6421 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
     6481 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac
     6541 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg
     6601 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga
     6661 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag
     6721 aacccaaggt cttgagccct tcgctaatgc gggaaagctc ttattcgggt gagatgggct
     6781 gggcaccatc tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg
     6841 tctgttgcgg gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga
     6901 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg
     6961 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc
     7021 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga
     7081 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt
     7141 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg
     7201 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gacttgtaaa ttgtccgcta
     7261 aattctggcc gtttttggct tttttgttag acaacatggg taaaaagcct gaactcaccg
     7321 cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc
     7381 tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc
     7441 tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg
     7501 catccgccgc gctcccgatt ccggaagtgc ttgacattgg ggagttcagc gagagcctga
     7561 cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac
     7621 tgcccgctgt tctgcagccg gtcgcggagg caatggatgc catcgctgcc gccgatctta
     7681 gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc
     7741 gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg
     7801 acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact
     7861 gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca
     7921 atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg
     7981 aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct
     8041 acttcgagcg gaggcatccg gagcttgcag gatcgccccg gctccgggcg tatatgctcc
     8101 gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt
     8161 gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac
     8221 aaatcgcccg cagaagcgcc gccgtctgga ccgatggctg tgtagaagta ctcgccgata
     8281 gtggaaaccg acgccccagc actcgtccga gggcaaagga ataagctagt atgtaagcct
     8341 agtcttagat aataaaatcg ctatccatcg aagatggatg tgtgttggtt ttttgtgtgt
     8401 gtaacgctag gcgcgcctgg tgtaccgaga acgatcctct cagtgcgagt ctcgacgatc
     8461 catatcgttg cttggcagtc agccagtcgg aatccagctt gggacccagg aagtccaatc
     8521 gtcagatatt gtactcaagc ctggtcacgg cagcgtaccg atctgtttaa acctagatat
     8581 tgatagtctg atcggtcaac gtataatcga gtcctagctt ttgcaaacat ctatcaagag
     8641 acaggatcag caggaggctt tcgcatgagt attcaacatt tccgtgtcgc ccttattccc
     8701 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8761 gatgctgaag atcagttggg tgcgcgagtg ggttacatcg aactggatct caacagcggt
     8821 aagatccttg agagttttcg ccccgaagaa cgctttccaa tgatgagcac ttttaaagtt
     8881 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     8941 atacactatt ctcagaatga cttggttgag tattcaccag tcacagaaaa gcatcttacg
     9001 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9061 gccaacttac ttctgacaac gattggagga ccgaaggagc taaccgcttt tttgcacaac
     9121 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9181 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caaccttgcg taaactatta
     9241 actggcgaac tacttactct agcttcccgg caacagttga tagactggat ggaggcggat
     9301 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9361 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9421 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9481 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaaccgat tctaggtgca
     9541 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgccag
     9601 attcagcaac ggatacggct tccccaactt gcccacttcc atacgtgtcc tccttaccag
     9661 aaatttatcc ttaagatccc gaatcgttta aactcgactc tggctctatc gaatctccgt
     9721 cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat cgaatctcgt
     9781 cagctatcgt cagcttacct ttttggcagc gatcgcggct cccgacatct tggaccatta
     9841 gctccacagg tatcttcttc cctctagtgg tcataacagc agcttcagct acctctcaat
     9901 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctatc aatcgttgcg
     9961 ttacacacac aaaaaaccaa cacacatcca tcttcgatgg atagcgattt tattatctaa
    10021 ctgctgatcg agtgtagcca gatctagtaa tcaattacgg ggtcattagt tcatagccca
    10081 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
    10141 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
    10201 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
    10261 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
    10321 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
    10381 gtcatcgcta ttaccatg
//
