LOCUS       sequence-30035-e         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Bonano VI, Oltean S, Garcia-Blanco MA
  TITLE     A protocol for imaging alternative splicing regulation in vivo using
            fluorescence reporters in transgenic mice.
  JOURNAL   Nat Protoc. 2007  . 2(9):2166-81.
  PUBMED    17853873
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2007  . 2(9):2166-81."
COMMENT     Sequence Label: pRint
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(239..257)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(289..309)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctg gctagcgttt aaacttaagc ttaccatggt ggcctcctcc
      121 gaggacgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      181 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      241 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      301 ttccagtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ctacaagaag
      361 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      421 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgagtatg
      481 gatccctcta aaagcgggca tgacttctag agtagtccag ggtttccgag ggtttccgtc
      541 gactcagctc gtctcgaggg cgtactaact
//
