LOCUS       sequence-300366-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Monitor global F-actin distribution.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T
  TITLE     Membrane-proximal F-actin restricts local membrane protrusions and 
            directs cell migration.
  JOURNAL   Science. 2020 Jun 12;368(6496):1205-1210. doi: 
            10.1126/science.aay7794.
  PUBMED    32527825
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-06-12- 12"; volume: "368"; 
            issue: "6496"; pages: "1205-1210"
COMMENT     Sequence Label: C1-F-tractin-mCherry
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      190..199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             196..906
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(341..359)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     609..628
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcaagctta tgggcatggc gcgaccacgg
       61 ggcgcggggc cctgcagccc cgggttggag cgggctccgc gccggagcgt cggggagctg
      121 cgcctgctct tcgaagcgcg ctgcgccgca gtcgccgccg cagcggccgc agggtcggat
      181 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
      241 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      301 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      361 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      421 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      481 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      541 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      601 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      661 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      721 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      781 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      841 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      901 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
      961 ttgctttaaa aaacctccca cacctccccc t
//
