LOCUS       sequence-300381-         351 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  membrane anchor for FKBP-FRB system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T
  TITLE     Membrane-proximal F-actin restricts local membrane protrusions and 
            directs cell migration.
  JOURNAL   Science. 2020 Jun 12;368(6496):1205-1210. doi: 
            10.1126/science.aay7794.
  PUBMED    32527825
REFERENCE   2  (bases 1 to 351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-06-12- 12"; volume: "368"; 
            issue: "6496"; pages: "1205-1210"
COMMENT     Sequence Label: pCAG-Lyn11-FRB
FEATURES             Location/Qualifiers
     source          1..351
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..351
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 atgggatgta taaaatcaaa agggaaagac agcgcgggag cagatagtgc tggtagtgct
       61 ggtagtgctg gtatcctctg gcatgagatg tggcatgaag gcctggaaga ggcatctcgt
      121 ttgtactttg gggaaaggaa cgtgaaaggc atgtttgagg tgctggagcc cttgcatgct
      181 atgatggaac ggggccccca gactctgaag gaaacatcct ttaatcaggc ctatggtcga
      241 gatttaatgg aggcccaaga gtggtgcagg aagtacatga aatcagggaa tgtcaaggac
      301 ctcctccaag cctgggacct ctattatcat gtgttccgac gaatctcaaa g
//
