LOCUS       sequence-300402-        4302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF10 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4302)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136074
FEATURES             Location/Qualifiers
     source          1..4302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(125..171)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    286..407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(323..342)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     377..396
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      570..579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(648..850)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(677..697)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        852..1155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    1236..1284
                     /note="synthetic polyadenylation signal"
     terminator      1302..1348
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1353..1424
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1576..1712)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1725..2516,2517..2585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2348..2367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3331..3919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3820..3839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4145..4216
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(join(4261..4302,1..95))
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
ORIGIN
        1 gcaagttggg gaagccgtat ccgttgctga atctggcata tgtgggagta taagacgcgc
       61 agcgtcgcat caggcatttt tttctgcgcc aatgccggta gatagcaatc cggcccgagg
      121 ggcacaaaaa acccctcaag acccgtttag aggccccaag gggttatgct acgataccga
      181 tagagatggg ccaataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      241 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      301 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      361 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtgag
      421 ctgaaggtac gctgtatctc agtcagtcaa gctagcttat gtgagattaa agttaactac
      481 atctacttgt gctatgtagt tacgagaatt cattctgcac aagagtagac tatatatcgt
      541 aaacggaaaa gcgaaaacgt ttatatagcc catggtggcg gccgctggcg ggtgtgtcga
      601 gtggatggta ggatcgacaa agatctgatc tgacggttca ctaaaccagc tctgcttata
      661 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      721 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      781 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      841 aaccgcatca gcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      901 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      961 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     1021 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     1081 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     1141 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     1201 tactagatct ggctacactc gatcagcagt tagataataa aatcgctatc catcgaagat
     1261 ggatgtgtgt tggttttttg tgtgtgtaac gcaacgattg atagcataac cccttggggc
     1321 ctctaaacgg gtcttgaggg gttttttgaa ttgagaggta gctgaagctg ctgttatgac
     1381 cactagaggg aagaagatac ctgtggagct aatggtccaa gatgtcggga gccgcgatcg
     1441 ctgccaaaaa ggtaagctga cgatagctga cgagattcga tgcccgacga gcaaatgagc
     1501 gccacggctg ttcgcgtaag ctcgaaacga cggagattcg atagagccag agtcgagttt
     1561 aaacgattcg ggatcttaag gataaatttc tggtaaggag gacacgtatg gaagtgggca
     1621 agttggggaa gccgtatccg ttgctgaatc tggcatatgt gggagtataa gacgcgcagc
     1681 gtcgcatcag gcattttttt ctgcgccaat gcacctagaa tcggttacca atgcttaatc
     1741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat caactgttgc
     1981 cgggaagcta gagtaagtag ttcgccagtt aatagtttac gcaaggttgt tgccattgct
     2041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2161 cctccaatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaatac
     2281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2341 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaagcgt
     2401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2461 actcgcgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2581 ctcatgcgaa agcctcctgc tgatcctgtc tcttgataga tgtttgcaaa agctaggact
     2641 cgattatacg ttgaccgatc agactatcaa tatctaggtt taaacagatc ggtacgctgc
     2701 cgtgaccagg cttgagtaca atatctgacg attggacttc ctgggtccca agctggattc
     2761 cgactggctg actgccaagc aacgatatgg atcgtcgaga ctcgcactga gaggatcgtt
     2821 ctcggtacac caggcgcgcc ccgatttcga tattcctcgg atctcggata gatcgggatt
     2881 gaggatgtag gttgaacttc tctcgggtga tcccgatctc ggatattcgg gattggagta
     2941 atacctggcg ataggcgctg ccaagcaata gccaaggcgc aaggccggcc aagagacttg
     3001 cgatagttcg ttcgagaggt agcctgcaag atggatcgga cgaaaggttt gaagattttc
     3061 cgacgagggc cttggagagt tcggaaattt aaatgcaact atggatgaac gaaatagaca
     3121 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3181 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3241 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3301 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3361 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3421 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3481 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3541 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3601 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3661 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3721 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3781 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3841 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3901 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3961 ctggcctttt gctcacatga tttaaatgaa actcgtaatc ggagaacggg tgcaatagca
     4021 aggaccagat cgaacttgac tgacgcaatt ctgagcgtag tcgaaaccca gacaagtcga
     4081 tctcgccaag tagctgactt gactggttaa ttaagtttcg atagcccaag gtaaccaatg
     4141 agattaggga gggaagaaag tttgatgaac tacttctgac ccctagtggt gtccagaaaa
     4201 gaccattaaa ggaatgtctt gatcgaaatc gagagatcgc ctgcaggata gctgacgacc
     4261 ttaaggataa atttctggta aggaggacac gtatggaagt gg
//
