LOCUS       sequence-300417-        8586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8586)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4046680
FEATURES             Location/Qualifiers
     source          1..8586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(320..1123)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     1474..1492
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(5367..5808)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(5373..5396)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    5691..5808
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     terminator      5875..5906
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        5907..6009
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(6010..6078,6079..6870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6228..6247)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             6871..7062
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      7086..7113
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(7125..7712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(7734..7763)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      join(7841..8586,1..237)
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 ctctcgcgtt gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta
       61 gcgctctcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
      121 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
      181 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttact
      241 cgggtaataa ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat
      301 ttacttataa tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc
      361 ctgcttttct gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc
      421 ttccaacaat aataatgtca gatcctgtag aaaccacatc atccacggtt ctatactgtt
      481 gacccaatgc atctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt
      541 aaccttcatc tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt
      601 cgctcttcgc aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc
      661 atgacaattc tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct
      721 gcttcaaacc gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc
      781 attctgctat tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag
      841 caaattttct gtcctcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct
      901 taactgtgcc ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa
      961 ttttgggacc taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg
     1021 aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac
     1081 taggatgagt agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc
     1141 tgcacgtttt tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa
     1201 cactacatat gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct
     1261 gttcggagat taccgaatca aaaaaatttc aaggaaaccg tgtactgcag tgcactagtg
     1321 gttcggcggc cgctttgtac agaaaaaaaa gaaaaatttg aaatataaat aacgttctta
     1381 atactaacat aactattaaa aaaaataaat agggacctag acttcaggtt gtctaactcc
     1441 ttccttttcg gttagagcgg atgtgggagg agggcgtgaa tgtaagcgtg acataactaa
     1501 ttacatgata tcgacaaagg aaaaggggcc tgtttacgtg tagtgcaatt taacaccctt
     1561 caatacaggt tcagagtcgt cttcatcgaa cttgcaacaa gaaccgcagc tgcaacaacc
     1621 ctttaaacaa gaacaacatg aggtcataca gcacaacatg atggtcacca taacaatagc
     1681 aatcagccct gcgataaaac ccaaccagat gtaccacggc catttaatgt attgttcgta
     1741 cttacctagt tcttggagat caatcaatga ctcgttcaag ttcttggcaa cctcatttaa
     1801 cctgtctatt tccttttgaa tgttaaccac ggaagcgtta atgccagata tgtcacctag
     1861 atcaacgtca ggggaggtgt gattcttaaa gtatttatcc aattcttcct tgaaggaatc
     1921 taattctggt tggagtggat cataaacggt gttgttgaca ataccgatga caacgtcaca
     1981 attaccggat acgaaggtgt tgtcagtagt aataatttgg ggctcgtaga agtttctttg
     2041 tgtgacgaac cagtgagtac cattagaaac gaaaacacct tctcttggga agtgagcttt
     2101 accatcgtga cagatggcag gagctgtagt gaagttcttt tcctgagccg gcacgtaagt
     2161 aacatgcaaa aagacgacac cgtgcggggc agattgggga aaggacatca aatgataacc
     2221 tttaccacag aagtcaacac gcttagactg acctaggaca cattcactca tcttggtcgc
     2281 ggctaaatta gcactagctc taatttcagc agcgcggata agttgttgag taacgtaggt
     2341 ttgtagcgat tgcaatcttc cggtgatcaa tctatcgatt tgtacttcgg cctcaacctt
     2401 atccaaccta gataagatat cgttgagtac ggaagaaatg gccccgaagt tagaggacaa
     2461 ctgttttacg agggtgttca aagcttgagc attctggtta acaacgtctt gtagtttacc
     2521 aagcgccgaa gcagtcgagg acaaagagtc ttgaatctta ccaatggccg agttgaattg
     2581 attagcgatt agcttttggt tttcatacag aacgttttga gtaactccta tcccgttgaa
     2641 tctgtaagcc atttgcatag caaatgggat ttgcaatgca gcaccagcac caaatgtcca
     2701 accagaagta atcgtcccag ccaagagcgc tgaggtatat tgagcgatca tttcatcagt
     2761 caatagtggg ggcaacacgg taagaccatt gaacttctgc gcacatatca agtctcgggc
     2821 tgcaatgtca cccaagcaat cgccatattg cttgatgaat ccagcgtcgg ctaacgtaac
     2881 tttattaaat aataaatctt cgataaacga tctcttggat ggctttgatg gatctggcaa
     2941 gatttggcta aagttgaaac cgccgaaatc cttaatgggt ggggtcttgt agatttgctt
     3001 gacttgggca aatacttcct gggtgttttt gtcttgttcg acagcaatac ccgtaagagc
     3061 acggttcaat tgtgtgcaga aggaaccgta ttgtaacaac aaattagaac actcagtaga
     3121 gtcaccacag atatacattg tacaatccac tgaggtcttt gtcatagaga caggtaagat
     3181 ttcagtagta acagaaatag tgaaattagt tggtatagca atagaattgt tactgtatgc
     3241 gactgagttt tcggcaccta ggctcatggt gtatgcaatg atactttgtg aagcaacact
     3301 tctagctctt cttggtgaat tagtctgtgt ttggtaggat gcacagattc cggcaccaat
     3361 tggaatgtca cattcatagg agttgttgac atgctcagca cctatcaaac aaccagctct
     3421 tgtttggaaa acattagaac cagtagagta gactctccaa gtaggggtta gttggtcagc
     3481 gtggatagca actggcactt ccgtacagtt gacgtcttgg tataaaacgg cgacctgatt
     3541 agaagtgttg gttccagggg taataacgct aacaccacca aaggaacatg gagtgatgtc
     3601 caaaatttcc aaagtttgag gatcccggac cgcatcggtc gtatctgcaa tatctctacc
     3661 gaattgttgg aatggcagga acttcttgtt ggattcggtc aatacgccag taccagtaag
     3721 accattgaaa ttgaagttga cacacttatt tttaaccaag ttcgtggact ttttgggacc
     3781 gcagactgta gctggggcat gcaatagttc aaaagacagg acaacaactc tatatggttg
     3841 gtaaccaact ccgttggttg gctgaaaacc gtaagattgt aaggggaagt aacagttaaa
     3901 accttcgacc ccattacaag gagtagaacc agcttgataa atctcggtac ttatgtctct
     3961 ttcgaaaggt ttcaaattag acttacgaaa taggcggtac aaatagttat agttcccacc
     4021 aaccttcgaa tccaagttgt tgctattcca agcgatcacg caaccggtga agtcgtctgg
     4081 caatttgtag ttgtaatcag caatcttacc ggtttgacct ggcgcgattt gtcttacttc
     4141 atcgcctcta atgacaaagg agtctgcgta aacattagtg aagcataagt cgttaagctt
     4201 ggttggcgaa accccgtaac atttgaaagt agagaaggaa gcactgttat atagtacaga
     4261 gtaatctgcc acacaattgg agattctctt acggttccaa gcgtacacag aagcaaacct
     4321 tgtggcatta aaaacctcac cgaatggaca caagttggtg atatttggga atctgactat
     4381 tgattcagtt ggttgaactc taaagttgct agtttggtat ataccctttt caactgtgaa
     4441 agattttagg gtgcacttag tttcgctcaa tggatccaaa gcacagtcta cagcatcggt
     4501 aattgtcccg ttttcattgt acttgagcag aaaggttctt ggctgcaaat aaccgacata
     4561 gtaggcagca gcacccgcag tccatccaga cgaggagtca ccaggagtta aatatgatcg
     4621 gtgtaaggct agcaaagttt gaaatctcgt aatgttgata ccaatcggca aatcaacgag
     4681 tggttccaaa gcagagaaac cctgaggtaa atctctaact aggttaattg gtgtgtgctt
     4741 agagtagatc ttaaagtaac cgtcgatatt tttgaagaca aattctctca agttcttgaa
     4801 gttgccttgc ttaccctcca aatccatcag gaaaggttga ctgacgtatt cgaaggtaca
     4861 gttgttagca cttgaataaa cccgaaattc ggattccatc cagcttttat tattcttatg
     4921 gtaataaaca cccaaaaacg ggtcgttaca gaattggaac tcacagactt taattacaac
     4981 attagtagcg ttgttgacaa ttaacaaaga ttgagtcttg gaatcaagcg tggttccaaa
     5041 gatccaacct ctaatgatgt tagacttttc tgtggaagcg aagtagacac cgtcattgaa
     5101 tggcaacact gggttatcga acctcttagt accattggta ccagaaacgt gtatggcgtg
     5161 aaaccaagta acgtttgaaa agaatggcaa gaacaaatct tgggtactat gtaaaaccga
     5221 agaacgaaat actttgtcgg ggtaatagac gcctctggtg aaagagttag tgtatgctgg
     5281 aggtagctgc gtgcgagttg tcagattaac gcattgggag ctcaccaatg gaagcaaaac
     5341 taaaaagacg aacattatag ttttttctcc ttgacgttaa agtatagagg tatattaaca
     5401 attttttgtt gatactttta tgacatttga ataagaagta atacaaactg aaaatgttga
     5461 aagtattagt taaagtggtt atgcagcttt tccatttata tatctgttaa tagatcaaaa
     5521 atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatca
     5581 tcctatggtt gttaatttga ttcgttaatt tgaaggtttg tggggccagg ttactgccaa
     5641 tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg
     5701 cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagacgagga cgcacggagg
     5761 agagtcttcc gtcggagggc tgtcgcccgc tcggcggctt ctaatccgta cttcaatata
     5821 gcaagcggcc gctctaactg accatttaaa tcatacctga cctccatagc agaaagtcaa
     5881 aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat
     5941 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
     6001 gaagctaaaa tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6061 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6121 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6181 ttacgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6241 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6301 aatgacttgg ttgagtactc accagtcaca gaaaagcatc tcacggatgg catgacagta
     6361 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6421 gcaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6481 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6541 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6601 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggatca
     6661 cttctgcgct cggccctccc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6721 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgcatcgta
     6781 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6841 ataggtgcct cactgattaa gcattggtaa gtgaccaaac aggaaaaaac cgcccttaac
     6901 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
     6961 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
     7021 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gatgagggcc caaatgtaat
     7081 cacctggctc accttcgggt gggcctttct gcgttgctgg cgtttttcca taggctccgc
     7141 ccccctgacg agcatcacaa aaatcgatgc tcaagtcaga ggtggcgaaa cccgacagga
     7201 ctataaagat accaggcgtt tacccctgga agctccctcg tgcgctctcc tgttccgacc
     7261 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7321 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7381 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7441 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7501 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7561 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttacctcgga aaaagagttg
     7621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgattttc taccgaagaa
     7741 aggcccaccc gtgaaggtga gccagtgagt tgattgcagt ccagttacgc tggagtcaag
     7801 cttgtaacac gcggccgctc gggaagttcc tattctctag ctagaaagta taggaacttc
     7861 agagcgcttt tgaaaaccaa aagcgctctg atgacgcact ttcaaaaaac caaaaacgca
     7921 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa
     7981 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc
     8041 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta
     8101 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga
     8161 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat
     8221 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc
     8281 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt
     8341 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag
     8401 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga
     8461 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat
     8521 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa
     8581 atagcg
//
