LOCUS       sequence-300430-        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136057
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     34..53
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(437..639)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(466..486)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        641..944
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    1025..1073
                     /note="synthetic polyadenylation signal"
     terminator      1091..1137
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1142..1213
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1365..1501)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1514..2305,2306..2374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2137..2156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3120..3708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3609..3628
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3934..4005
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4050..4186)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(4216..4262)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    join(4377..4434,1..64)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4414..4433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
       61 cttatcatgt ctgtgagctg aaggtacgct gtatctcagt cagtcaagct agcttataag
      121 gttgccctgt tgtccagcat ttcttcacaa agcttgttta tgtctacagc accctgcatg
      181 gaaagcaaaa cagaaagtag tgaaaccatt ttttcaaagg cttcagtagt atctttagct
      241 aagagaatgt cattgtgtaa ctggacacat tgagcccaca atttagatga tgattctact
      301 ctgagttgtt gcaaaactga gagtaagact actgatgtgc actttacatc tgacattttc
      361 atggtggcgg ccgctggcgg gtgtgtcgag tggatggtag gatcgacaaa gatctgatct
      421 gacggttcac taaaccagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
      481 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
      541 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
      601 gctatccacg cccattgatg tactgccaaa accgcatcag catggtaata gcgatgacta
      661 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
      721 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
      781 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
      841 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
      901 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
      961 ggctatgaac taatgacccc gtaattgatt actagatctg gctacactcg atcagcagtt
     1021 agataataaa atcgctatcc atcgaagatg gatgtgtgtt ggttttttgt gtgtgtaacg
     1081 caacgattga tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgaat
     1141 tgagaggtag ctgaagctgc tgttatgacc actagaggga agaagatacc tgtggagcta
     1201 atggtccaag atgtcgggag ccgcgatcgc tgccaaaaag gtaagctgac gatagctgac
     1261 gagattcgat gcccgacgag caaatgagcg ccacggctgt tcgcgtaagc tcgaaacgac
     1321 ggagattcga tagagccaga gtcgagttta aacgattcgg gatcttaagg ataaatttct
     1381 ggtaaggagg acacgtatgg aagtgggcaa gttggggaag ccgtatccgt tgctgaatct
     1441 ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg catttttttc tgcgccaatg
     1501 cacctagaat cggttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     1561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     1621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1741 ccgcctccat ccagtctatc aactgttgcc gggaagctag agtaagtagt tcgccagtta
     1801 atagtttacg caaggttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1921 tgtgcaaaaa agcggttagc tccttcggtc ctccaatcgt tgtcagaagt aagttggccg
     1981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2041 taagatgctt ttctgtgact ggtgaatact caaccaagtc attctgagaa tagtgtatgc
     2101 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     2161 ctttaaaagt gctcatcatt ggaaagcgtt cttcggggcg aaaactctca aggatcttac
     2221 cgctgttgag atccagttcg atgtaaccca ctcgcgcacc caactgatct tcagcatctt
     2281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     2341 gaataagggc gacacggaaa tgttgaatac tcatgcgaaa gcctcctgct gatcctgtct
     2401 cttgatagat gtttgcaaaa gctaggactc gattatacgt tgaccgatca gactatcaat
     2461 atctaggttt aaacagatcg gtacgctgcc gtgaccaggc ttgagtacaa tatctgacga
     2521 ttggacttcc tgggtcccaa gctggattcc gactggctga ctgccaagca acgatatgga
     2581 tcgtcgagac tcgcactgag aggatcgttc tcggtacacc aggcgcgccc cgatttcgat
     2641 attcctcgga tctcggatag atcgggattg aggatgtagg ttgaacttct ctcgggtgat
     2701 cccgatctcg gatattcggg attggagtaa tacctggcga taggcgctgc caagcaatag
     2761 ccaaggcgca aggccggcca agagacttgc gatagttcgt tcgagaggta gcctgcaaga
     2821 tggatcggac gaaaggtttg aagattttcc gacgagggcc ttggagagtt cggaaattta
     2881 aatgcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2941 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     3001 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3061 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3121 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3181 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3241 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3301 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3361 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3421 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3481 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3541 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3601 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3661 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3721 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgat ttaaatgaaa
     3781 ctcgtaatcg gagaacgggt gcaatagcaa ggaccagatc gaacttgact gacgcaattc
     3841 tgagcgtagt cgaaacccag acaagtcgat ctcgccaagt agctgacttg actggttaat
     3901 taagtttcga tagcccaagg taaccaatga gattagggag ggaagaaagt ttgatgaact
     3961 acttctgacc cctagtggtg tccagaaaag accattaaag gaatgtcttg atcgaaatcg
     4021 agagatcgcc tgcaggatag ctgacgacct taaggataaa tttctggtaa ggaggacacg
     4081 tatggaagtg ggcaagttgg ggaagccgta tccgttgctg aatctggcat atgtgggagt
     4141 ataagacgcg cagcgtcgca tcaggcattt ttttctgcgc caatgccggt agatagcaat
     4201 ccggcccgag gggcacaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     4261 tacgataccg atagagatgg gccaatacca catttgtaga ggttttactt gctttaaaaa
     4321 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4381 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttca
//
