LOCUS       sequence-300440-        4371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF6 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4371)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136072
FEATURES             Location/Qualifiers
     source          1..4371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    20..68
                     /note="synthetic polyadenylation signal"
     terminator      86..132
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    137..208
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(360..496)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(509..1300,1301..1369))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1132..1151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2115..2703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2604..2623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2929..3000
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(3045..3181)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(3211..3257)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3372..3493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3409..3428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3463..3482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(3803..4005)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3832..3852)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4007..4310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 actcgatcag cagttagata ataaaatcgc tatccatcga agatggatgt gtgttggttt
       61 tttgtgtgtg taacgcaacg attgatagca taaccccttg gggcctctaa acgggtcttg
      121 aggggttttt tgaattgaga ggtagctgaa gctgctgtta tgaccactag agggaagaag
      181 atacctgtgg agctaatggt ccaagatgtc gggagccgcg atcgctgcca aaaaggtaag
      241 ctgacgatag ctgacgagat tcgatgcccg acgagcaaat gagcgccacg gctgttcgcg
      301 taagctcgaa acgacggaga ttcgatagag ccagagtcga gtttaaacga ttcgggatct
      361 taaggataaa tttctggtaa ggaggacacg tatggaagtg ggcaagttgg ggaagccgta
      421 tccgttgctg aatctggcat atgtgggagt ataagacgcg cagcgtcgca tcaggcattt
      481 ttttctgcgc caatgcacct agaatcggtt accaatgctt aatcagtgag gcacctatct
      541 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      601 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      661 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      721 gtcctgcaac tttatccgcc tccatccagt ctatcaactg ttgccgggaa gctagagtaa
      781 gtagttcgcc agttaatagt ttacgcaagg ttgttgccat tgctacaggc atcgtggtgt
      841 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      901 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctcca atcgttgtca
      961 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1021 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga atactcaacc aagtcattct
     1081 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1141 cgccacatag cagaacttta aaagtgctca tcattggaaa gcgttcttcg gggcgaaaac
     1201 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgc gcacccaact
     1261 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1321 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcatg cgaaagcctc
     1381 ctgctgatcc tgtctcttga tagatgtttg caaaagctag gactcgatta tacgttgacc
     1441 gatcagacta tcaatatcta ggtttaaaca gatcggtacg ctgccgtgac caggcttgag
     1501 tacaatatct gacgattgga cttcctgggt cccaagctgg attccgactg gctgactgcc
     1561 aagcaacgat atggatcgtc gagactcgca ctgagaggat cgttctcggt acaccaggcg
     1621 cgccccgatt tcgatattcc tcggatctcg gatagatcgg gattgaggat gtaggttgaa
     1681 cttctctcgg gtgatcccga tctcggatat tcgggattgg agtaatacct ggcgataggc
     1741 gctgccaagc aatagccaag gcgcaaggcc ggccaagaga cttgcgatag ttcgttcgag
     1801 aggtagcctg caagatggat cggacgaaag gtttgaagat tttccgacga gggccttgga
     1861 gagttcggaa atttaaatgc aactatggat gaacgaaata gacagatcgc tgagataggt
     1921 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2761 atgatttaaa tgaaactcgt aatcggagaa cgggtgcaat agcaaggacc agatcgaact
     2821 tgactgacgc aattctgagc gtagtcgaaa cccagacaag tcgatctcgc caagtagctg
     2881 acttgactgg ttaattaagt ttcgatagcc caaggtaacc aatgagatta gggagggaag
     2941 aaagtttgat gaactacttc tgacccctag tggtgtccag aaaagaccat taaaggaatg
     3001 tcttgatcga aatcgagaga tcgcctgcag gatagctgac gaccttaagg ataaatttct
     3061 ggtaaggagg acacgtatgg aagtgggcaa gttggggaag ccgtatccgt tgctgaatct
     3121 ggcatatgtg ggagtataag acgcgcagcg tcgcatcagg catttttttc tgcgccaatg
     3181 ccggtagata gcaatccggc ccgaggggca caaaaaaccc ctcaagaccc gtttagaggc
     3241 cccaaggggt tatgctacga taccgataga gatgggccaa taccacattt gtagaggttt
     3301 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     3361 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     3421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     3481 tcaatgtatc ttatcatgtc tgtgagctga aggtacgctg tatctcagtc agtcaagcta
     3541 gcttagtcaa tctccattgg ctgttcctca tccagttggg aatatttatt ctcagttaaa
     3601 gacttactaa ggttttttat gatgagatta atgatgtagt ccaggttcca aatgctaacc
     3661 ttgaaggttc gcatgataat tagcagtatt tctgcgatcg tgacctgaaa atccaccaag
     3721 tggaacatgg tggcggccgc tggcgggtgt gtcgagtgga tggtaggatc gacaaagatc
     3781 tgatctgacg gttcactaaa ccagctctgc ttatatagac ctcccaccgt acacgcctac
     3841 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     3901 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     3961 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcagcatg gtaatagcga
     4021 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     4081 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     4141 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     4201 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     4261 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     4321 tatatgggct atgaactaat gaccccgtaa ttgattacta gatctggcta c
//
