LOCUS       sequence-300442-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136070
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(684..752,753..1544)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(902..921)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      1557..1693
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    1845..1916
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1921..1967)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    1985..2033
                     /note="synthetic polyadenylation signal"
     enhancer        2114..2417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2419..2621
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2572..2592
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2877..2998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2888..2907)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2942..2961
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      3113..3159
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3189..3325
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3370..3441
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(3667..4255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3747..3766)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
       61 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcatttaa
      181 atttccgaac tctccaaggc cctcgtcgga aaatcttcaa acctttcgtc cgatccatct
      241 tgcaggctac ctctcgaacg aactatcgca agtctcttgg ccggccttgc gccttggcta
      301 ttgcttggca gcgcctatcg ccaggtatta ctccaatccc gaatatccga gatcgggatc
      361 acccgagaga agttcaacct acatcctcaa tcccgatcta tccgagatcc gaggaatatc
      421 gaaatcgggg cgcgcctggt gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc
      481 atatcgttgc ttggcagtca gccagtcgga atccagcttg ggacccagga agtccaatcg
      541 tcagatattg tactcaagcc tggtcacggc agcgtaccga tctgtttaaa cctagatatt
      601 gatagtctga tcggtcaacg tataatcgag tcctagcttt tgcaaacatc tatcaagaga
      661 caggatcagc aggaggcttt cgcatgagta ttcaacattt ccgtgtcgcc cttattccct
      721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      781 atgctgaaga tcagttgggt gcgcgagtgg gttacatcga actggatctc aacagcggta
      841 agatccttga gagttttcgc cccgaagaac gctttccaat gatgagcact tttaaagttc
      901 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      961 tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg
     1021 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1081 ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca
     1141 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1201 acgacgagcg tgacaccacg atgcctgtag caatggcaac aaccttgcgt aaactattaa
     1261 ctggcgaact acttactcta gcttcccggc aacagttgat agactggatg gaggcggata
     1321 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1381 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     1441 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1501 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaaccgatt ctaggtgcat
     1561 tggcgcagaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
     1621 ttcagcaacg gatacggctt ccccaacttg cccacttcca tacgtgtcct ccttaccaga
     1681 aatttatcct taagatcccg aatcgtttaa actcgactct ggctctatcg aatctccgtc
     1741 gtttcgagct tacgcgaaca gccgtggcgc tcatttgctc gtcgggcatc gaatctcgtc
     1801 agctatcgtc agcttacctt tttggcagcg atcgcggctc ccgacatctt ggaccattag
     1861 ctccacaggt atcttcttcc ctctagtggt cataacagca gcttcagcta cctctcaatt
     1921 caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt tatgctatca atcgttgcgt
     1981 tacacacaca aaaaaccaac acacatccat cttcgatgga tagcgatttt attatctaac
     2041 tgctgatcga gtgtagccag atctagtaat caattacggg gtcattagtt catagcccat
     2101 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2161 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2221 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2281 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2341 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2401 tcatcgctat taccatgctg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2461 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2521 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2581 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     2641 tcagatcttt gtcgatccta ccatccactc gacacacccg ccagcggccg ccaccatgat
     2701 agaactatcg ctcatcgact tttatctgtg cttcttggct ttcctgcttt ttctggtgct
     2761 cattatgcta atcatttttt ggttcagtct ggagttacaa gatcataatg agacatgtca
     2821 cgcctaagct agcttgactg actgagatac agcgtacctt cagctcacag acatgataag
     2881 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     2941 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3001 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3061 aagcaagtaa aacctctaca aatgtggtat tggcccatct ctatcggtat cgtagcataa
     3121 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt gcccctcggg ccggattgct
     3181 atctaccggc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc ttatactccc
     3241 acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc catacgtgtc
     3301 ctccttacca gaaatttatc cttaaggtcg tcagctatcc tgcaggcgat ctctcgattt
     3361 cgatcaagac attcctttaa tggtcttttc tggacaccac taggggtcag aagtagttca
     3421 tcaaactttc ttccctccct aatctcattg gttaccttgg gctatcgaaa cttaattaac
     3481 cagtcaagtc agctacttgg cgagatcgac ttgtctgggt ttcgactacg ctcagaattg
     3541 cgtcagtcaa gttcgatctg gtccttgcta ttgcacccgt tctccgatta cgagtttcat
     3601 ttaaatcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3661 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3721 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3781 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3841 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3901 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3961 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4021 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4081 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4141 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4201 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4261 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagg
//
