LOCUS       sequence-300452-        4224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4224)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136048
FEATURES             Location/Qualifiers
     source          1..4224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(125..713)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(205..224)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(1459..1527,1528..2319)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1677..1696)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2332..2468
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    2620..2691
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(2696..2742)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    2760..2808
                     /note="synthetic polyadenylation signal"
     enhancer        2889..3192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3194..3396
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3347..3367
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3465..3474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3559..3680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3570..3589)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3624..3643
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      3795..3841
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3871..4007
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4052..4123
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
ORIGIN
        1 tcagtcaagt tcgatctggt ccttgctatt gcacccgttc tccgattacg agtttcattt
       61 aaatcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      541 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgca tttaaatttc
      961 cgaactctcc aaggccctcg tcggaaaatc ttcaaacctt tcgtccgatc catcttgcag
     1021 gctacctctc gaacgaacta tcgcaagtct cttggccggc cttgcgcctt ggctattgct
     1081 tggcagcgcc tatcgccagg tattactcca atcccgaata tccgagatcg ggatcacccg
     1141 agagaagttc aacctacatc ctcaatcccg atctatccga gatccgagga atatcgaaat
     1201 cggggcgcgc ctggtgtacc gagaacgatc ctctcagtgc gagtctcgac gatccatatc
     1261 gttgcttggc agtcagccag tcggaatcca gcttgggacc caggaagtcc aatcgtcaga
     1321 tattgtactc aagcctggtc acggcagcgt accgatctgt ttaaacctag atattgatag
     1381 tctgatcggt caacgtataa tcgagtccta gcttttgcaa acatctatca agagacagga
     1441 tcagcaggag gctttcgcat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1501 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1561 gaagatcagt tgggtgcgcg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1621 cttgagagtt ttcgccccga agaacgcttt ccaatgatga gcacttttaa agttctgcta
     1681 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1741 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc
     1801 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     1861 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     1921 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     1981 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacct tgcgtaaact attaactggc
     2041 gaactactta ctctagcttc ccggcaacag ttgatagact ggatggaggc ggataaagtt
     2101 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     2161 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2221 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     2281 atcgctgaga taggtgcctc actgattaag cattggtaac cgattctagg tgcattggcg
     2341 cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag
     2401 caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt
     2461 atccttaaga tcccgaatcg tttaaactcg actctggctc tatcgaatct ccgtcgtttc
     2521 gagcttacgc gaacagccgt ggcgctcatt tgctcgtcgg gcatcgaatc tcgtcagcta
     2581 tcgtcagctt acctttttgg cagcgatcgc ggctcccgac atcttggacc attagctcca
     2641 caggtatctt cttccctcta gtggtcataa cagcagcttc agctacctct caattcaaaa
     2701 aacccctcaa gacccgttta gaggccccaa ggggttatgc tatcaatcgt tgcgttacac
     2761 acacaaaaaa ccaacacaca tccatcttcg atggatagcg attttattat ctaactgctg
     2821 atcgagtgta gccagatcta gtaatcaatt acggggtcat tagttcatag cccatatatg
     2881 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2941 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3001 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3061 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3121 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3181 gctattacca tgctgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3241 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3301 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3361 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatcaga
     3421 tctttgtcga tcctaccatc cactcgacac acccgccagc ggccgccacc atggctgatg
     3481 cacaatcgtt tttaaacggg tttgcgtaag ctagcttgac tgactgagat acagcgtacc
     3541 ttcagctcac agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3601 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3661 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3721 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt attggcccat
     3781 ctctatcggt atcgtagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     3841 gtgcccctcg ggccggattg ctatctaccg gcattggcgc agaaaaaaat gcctgatgcg
     3901 acgctgcgcg tcttatactc ccacatatgc cagattcagc aacggatacg gcttccccaa
     3961 cttgcccact tccatacgtg tcctccttac cagaaattta tccttaaggt cgtcagctat
     4021 cctgcaggcg atctctcgat ttcgatcaag acattccttt aatggtcttt tctggacacc
     4081 actaggggtc agaagtagtt catcaaactt tcttccctcc ctaatctcat tggttacctt
     4141 gggctatcga aacttaatta accagtcaag tcagctactt ggcgagatcg acttgtctgg
     4201 gtttcgacta cgctcagaat tgcg
//
