LOCUS       sequence-300461-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate hACE2 stable expressing cell line.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  TITLE     De Francesco Lab Plasmids
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLENTI_hACE2_PURO
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    113..134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(149..154,155..172,173..179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    187..203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     192..214
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     211..227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     211..227
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     246..266
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        248..266
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        294..520
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             521..701
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    748..873
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1366..1599
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1784..1828
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2095..2211
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2234..2537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2538..2741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2692..2712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2738..2762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5300..5320)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5305..5634
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5485..5620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5644..6243
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5644..5663)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6140..6160
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6310..6898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6363..6383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6781..6792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6985..7218
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7290..7411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7327..7346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7381..7400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7451..7586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7606..7625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7607..7625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7635..7652)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7635..7651)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7644..7666)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7793..8248
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7880..7899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8090..8111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8274..8378
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8379..8447,8448..9239)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8597..8616)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9410..9998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9899..9918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10152..10169
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
       61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
      241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
      301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
      361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
      421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
      481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag
     2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagaatg
     2821 tcaagctctt cctggctcct tctcagcctt gttgctgtaa ctgctgctca gtccaccatt
     2881 gaggaacagg ccaagacatt tttggacaag tttaaccacg aagccgaaga cctgttctat
     2941 caaagttcac ttgcttcttg gaattataac accaatatta ctgaagagaa tgtccaaaac
     3001 atgaataatg ctggggacaa atggtctgcc tttttaaagg aacagtccac acttgcccaa
     3061 atgtatccac tacaagaaat tcagaatctc acagtcaagc ttcagctgca ggctcttcag
     3121 caaaatgggt cttcagtgct ctcagaagac aagagcaaac ggttgaacac aattctaaat
     3181 acaatgagca ccatctacag tactggaaaa gtttgtaacc cagataatcc acaagaatgc
     3241 ttattacttg aaccaggttt gaatgaaata atggcaaaca gtttagacta caatgagagg
     3301 ctctgggctt gggaaagctg gagatctgag gtcggcaagc agctgaggcc attatatgaa
     3361 gagtatgtgg tcttgaaaaa tgagatggca agagcaaatc attatgagga ctatggggat
     3421 tattggagag gagactatga agtaaatggg gtagatggct atgactacag ccgcggccag
     3481 ttgattgaag atgtggaaca tacctttgaa gagattaaac cattatatga acatcttcat
     3541 gcctatgtga gggcaaagtt gatgaatgcc tatccttcct atatcagtcc aattggatgc
     3601 ctccctgctc atttgcttgg tgatatgtgg ggtagatttt ggacaaatct gtactctttg
     3661 acagttccct ttggacagaa accaaacata gatgttactg atgcaatggt ggaccaggcc
     3721 tgggatgcac agagaatatt caaggaggcc gagaagttct ttgtatctgt tggtcttcct
     3781 aatatgactc aaggattctg ggaaaattcc atgctaacgg acccaggaaa tgttcagaaa
     3841 gcagtctgcc atcccacagc ttgggacctg gggaagggcg acttcaggat ccttatgtgc
     3901 acaaaggtga caatggacga cttcctgaca gctcatcatg agatggggca tatccagtat
     3961 gatatggcat atgctgcaca accttttctg ctaagaaatg gagctaatga aggattccat
     4021 gaagctgttg gggaaatcat gtcactttct gcagccacac ctaagcattt aaaatccatt
     4081 ggtcttctgt cacccgattt tcaagaagac aatgaaacag aaataaactt cctgctcaaa
     4141 caagcactca cgattgttgg gactctgcca tttacttaca tgttagagaa gtggaggtgg
     4201 atggtcttta aaggggaaat tcccaaagac cagtggatga aaaagtggtg ggagatgaag
     4261 cgagagatag ttggggtggt ggaacctgtg ccccatgatg aaacatactg tgaccccgca
     4321 tctctgttcc atgtttctaa tgattactca ttcattcgat attacacaag gaccctttac
     4381 caattccagt ttcaagaagc actttgtcaa gcagctaaac atgaaggccc tctgcacaaa
     4441 tgtgacatct caaactctac agaagctgga cagaaactgt tcaatatgct gaggcttgga
     4501 aaatcagaac cctggaccct agcattggaa aatgttgtag gagcaaagaa catgaatgta
     4561 aggccactgc tcaactactt tgagccctta tttacctggc tgaaagacca gaacaagaat
     4621 tcttttgtgg gatggagtac cgactggagt ccatatgcag accaaagcat caaagtgagg
     4681 ataagcctaa aatcagctct tggagataaa gcatatgaat ggaacgacaa tgaaatgtac
     4741 ctgttccgat catctgttgc atatgctatg aggcagtact ttttaaaagt aaaaaatcag
     4801 atgattcttt ttggggagga ggatgtgcga gtggctaatt tgaaaccaag aatctccttt
     4861 aatttctttg tcactgcacc taaaaatgtg tctgatatca ttcctagaac tgaagttgaa
     4921 aaggccatca ggatgtcccg gagccgtatc aatgatgctt tccgtctgaa tgacaacagc
     4981 ctagagtttc tggggataca gccaacactt ggacctccta accagccccc tgtttccata
     5041 tggctgattg tttttggagt tgtgatggga gtgatagtgg ttggcattgt catcctgatc
     5101 ttcactggga tcagagatcg gaagaagaaa aataaagcaa gaagtggaga aaatccttat
     5161 gcctccatcg atattagcaa aggagaaaat aatccaggat tccaaaacac tgatgatgtt
     5221 cagacctcct ttggtaccga gacctcccag gtggcgcccg cttaaactag ttaacgcgtc
     5281 gaccagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5461 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     5641 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     5701 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     5761 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     5821 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     5881 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     5941 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6001 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6061 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6121 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6181 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6241 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     6301 ccagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     6721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6901 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7561 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     7621 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     7681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     7741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     7801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8221 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     8281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10141 cgaccgagcg cagcgagtca gtgagcgagg aag
//
