LOCUS       sequence-300462-       11020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector to generate hACE2 stable expressing cell line, 
            Hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11020)
  AUTHORS   Notarbartolo S, Ranzani V, Bandera A, Gruarin P, Bevilacqua V, 
            Putignano AR, Gobbini A, Galeota E, Manara C, Bombaci M, Pesce E, 
            Zagato E, Favalli A, Sarnicola ML, Curti S, Crosti M, Martinovic M, 
            Fabbris T, Marini F, Donnici L, Lorenzo M, Mancino M, Ungaro R, 
            Lombardi A, Mangioni D, Muscatello A, Aliberti S, Blasi F, De Feo T,
            Prati D, Manganaro L, Granucci F, Lanzavecchia A, De Francesco R, 
            Gori A, Grifantini R, Abrignani S
  TITLE     Integrated longitudinal immunophenotypic, transcriptional and 
            repertoire analyses delineate immune responses in COVID-19 patients.
  JOURNAL   Sci Immunol. 2021 Aug 10;6(62). pii: 6/62/eabg5021. doi: 
            10.1126/sciimmunol.abg5021.
  PUBMED    34376481
REFERENCE   2  (bases 1 to 11020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-08-10"; volume: "6(62). pii: 6/62/eabg5021. 
            doi"; pages: " 10.1126/sciimmunol.abg5021"
COMMENT     Sequence Label: pLENTI_hACE2_HygR
FEATURES             Location/Qualifiers
     source          1..11020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    228..249
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(264..269,270..287,288..294)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    302..318
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     307..329
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     326..342
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     326..342
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     361..381
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        363..381
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        409..635
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             636..816
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    863..988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1481..1714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1899..1943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2210..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2349..2652
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2653..2856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2807..2827
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2853..2877
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    5387..5975
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5440..5460)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5858..5869)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6031..6530
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6066..6089)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6463..6482
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6548..7573
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6548..7573
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7947..8180
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8252..8373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8289..8308)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8343..8362
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8413..8548
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8568..8587)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8569..8587)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8597..8614)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8597..8613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8606..8628)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8755..9210
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8842..8861)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9052..9073
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9236..9340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9341..9409,9410..10201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9559..9578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10372..10960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10861..10880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
      361 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
      421 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      481 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      541 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
      601 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
      661 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
      721 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      781 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
      841 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
      901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
      961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     1381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     1441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     1501 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     1561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     1621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     1681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     1741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     1801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     1861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     1921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     1981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     2041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     2101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     2161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     2221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2281 tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg ataagcttgg
     2341 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2401 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2461 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     2521 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     2581 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     2641 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     2701 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     2761 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     2821 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2881 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaatgtcaag
     2941 ctcttcctgg ctccttctca gccttgttgc tgtaactgct gctcagtcca ccattgagga
     3001 acaggccaag acatttttgg acaagtttaa ccacgaagcc gaagacctgt tctatcaaag
     3061 ttcacttgct tcttggaatt ataacaccaa tattactgaa gagaatgtcc aaaacatgaa
     3121 taatgctggg gacaaatggt ctgccttttt aaaggaacag tccacacttg cccaaatgta
     3181 tccactacaa gaaattcaga atctcacagt caagcttcag ctgcaggctc ttcagcaaaa
     3241 tgggtcttca gtgctctcag aagacaagag caaacggttg aacacaattc taaatacaat
     3301 gagcaccatc tacagtactg gaaaagtttg taacccagat aatccacaag aatgcttatt
     3361 acttgaacca ggtttgaatg aaataatggc aaacagttta gactacaatg agaggctctg
     3421 ggcttgggaa agctggagat ctgaggtcgg caagcagctg aggccattat atgaagagta
     3481 tgtggtcttg aaaaatgaga tggcaagagc aaatcattat gaggactatg gggattattg
     3541 gagaggagac tatgaagtaa atggggtaga tggctatgac tacagccgcg gccagttgat
     3601 tgaagatgtg gaacatacct ttgaagagat taaaccatta tatgaacatc ttcatgccta
     3661 tgtgagggca aagttgatga atgcctatcc ttcctatatc agtccaattg gatgcctccc
     3721 tgctcatttg cttggtgata tgtggggtag attttggaca aatctgtact ctttgacagt
     3781 tccctttgga cagaaaccaa acatagatgt tactgatgca atggtggacc aggcctggga
     3841 tgcacagaga atattcaagg aggccgagaa gttctttgta tctgttggtc ttcctaatat
     3901 gactcaagga ttctgggaaa attccatgct aacggaccca ggaaatgttc agaaagcagt
     3961 ctgccatccc acagcttggg acctggggaa gggcgacttc aggatcctta tgtgcacaaa
     4021 ggtgacaatg gacgacttcc tgacagctca tcatgagatg gggcatatcc agtatgatat
     4081 ggcatatgct gcacaacctt ttctgctaag aaatggagct aatgaaggat tccatgaagc
     4141 tgttggggaa atcatgtcac tttctgcagc cacacctaag catttaaaat ccattggtct
     4201 tctgtcaccc gattttcaag aagacaatga aacagaaata aacttcctgc tcaaacaagc
     4261 actcacgatt gttgggactc tgccatttac ttacatgtta gagaagtgga ggtggatggt
     4321 ctttaaaggg gaaattccca aagaccagtg gatgaaaaag tggtgggaga tgaagcgaga
     4381 gatagttggg gtggtggaac ctgtgcccca tgatgaaaca tactgtgacc ccgcatctct
     4441 gttccatgtt tctaatgatt actcattcat tcgatattac acaaggaccc tttaccaatt
     4501 ccagtttcaa gaagcacttt gtcaagcagc taaacatgaa ggccctctgc acaaatgtga
     4561 catctcaaac tctacagaag ctggacagaa actgttcaat atgctgaggc ttggaaaatc
     4621 agaaccctgg accctagcat tggaaaatgt tgtaggagca aagaacatga atgtaaggcc
     4681 actgctcaac tactttgagc ccttatttac ctggctgaaa gaccagaaca agaattcttt
     4741 tgtgggatgg agtaccgact ggagtccata tgcagaccaa agcatcaaag tgaggataag
     4801 cctaaaatca gctcttggag ataaagcata tgaatggaac gacaatgaaa tgtacctgtt
     4861 ccgatcatct gttgcatatg ctatgaggca gtacttttta aaagtaaaaa atcagatgat
     4921 tctttttggg gaggaggatg tgcgagtggc taatttgaaa ccaagaatct cctttaattt
     4981 ctttgtcact gcacctaaaa atgtgtctga tatcattcct agaactgaag ttgaaaaggc
     5041 catcaggatg tcccggagcc gtatcaatga tgctttccgt ctgaatgaca acagcctaga
     5101 gtttctgggg atacagccaa cacttggacc tcctaaccag ccccctgttt ccatatggct
     5161 gattgttttt ggagttgtga tgggagtgat agtggttggc attgtcatcc tgatcttcac
     5221 tgggatcaga gatcggaaga agaaaaataa agcaagaagt ggagaaaatc cttatgcctc
     5281 catcgatatt agcaaaggag aaaataatcc aggattccaa aacactgatg atgttcagac
     5341 ctcctttggt accgagacct cccaggtggc gcccgcttaa gtcgacaatc aacctctgga
     5401 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5461 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5521 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5581 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5641 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5701 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5761 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5821 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5881 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5941 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctgcaga tatccggact
     6001 agtgatctct cgaggttaac gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     6061 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     6121 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     6181 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     6241 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     6301 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     6361 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     6421 tcaggggcgg ggcgggcgcc cgaaggccct ccggaggccc ggcattctgc acgcttcaaa
     6481 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc
     6541 cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     6601 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     6661 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     6721 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     6781 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     6841 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     6901 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     6961 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     7021 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     7081 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     7141 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     7201 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     7261 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     7321 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     7381 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     7441 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     7501 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     7561 ggcaaaggaa tagagtagat gccgaccgaa caagagctga tttcgagaac gcctcagcca
     7621 gcaactcgcg cgagcctagc aaggcaaatg cgagagaacg gccttacgct tggtggcaca
     7681 gttctcgtcc acagttcgct aagctcgctc ggctgggtcg cgggagggcc ggtcgcagtg
     7741 attcaggccc ttctggattg tgttggtccc cagggcacga ttgtcatgcc cacgcactcg
     7801 ggtgatctga ctgatcccgc agattggaga tcgccgcccg tgcctgccga ttgggtgcag
     7861 atccgtcgag ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7921 ccacttttta aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga
     7981 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8041 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     8101 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     8161 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     8221 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     8281 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8341 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     8401 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8461 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8521 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     8581 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     8641 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     8701 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     8761 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     8821 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     8881 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     8941 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     9001 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     9061 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     9121 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     9181 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     9241 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     9301 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     9361 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     9421 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     9481 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9541 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9601 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9661 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9721 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9781 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9841 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9901 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9961 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    10021 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    10081 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    10141 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    10201 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    10261 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10321 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10381 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10441 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10501 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10561 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10621 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10681 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10741 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10801 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10861 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10921 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10981 tttacggttc ctggcctttt gctggccttt tgctcacatg
//
