LOCUS       sequence-300463-        9118 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of spike with C-term deletion of 19 aa, used to generate 
            high efficiency SARS-CoV-2-pseudotyped lentiviral particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Conforti A, Marra E, Palombo F, Roscilli G, Rava M, Fumagalli V, 
            Muzi A, Maffei M, Luberto L, Lione L, Salvatori E, Compagnone M, 
            Pinto E, Pavoni E, Bucci F, Vitagliano G, Stoppoloni D, Pacello ML, 
            Cappelletti M, Ferrara FF, D'Acunto E, Chiarini V, Arriga R, Nyska 
            A, Di Lucia P, Marotta D, Bono E, Giustini L, Sala E, Perucchini C, 
            Paterson J, Ryan KA, Challis AR, Matusali G, Colavita F, Caselli G, 
            Criscuolo E, Clementi N, Mancini N, Gross R, Seidel A, Wettstein L, 
            Munch J, Donnici L, Conti M, De Francesco R, Kuka M, Ciliberto G, 
            Castilletti C, Capobianchi MR, Ippolito G, Guidotti LG, Rovati L, 
            Iannacone M, Aurisicchio L
  TITLE     COVID-eVax, an electroporated DNA vaccine candidate encoding the 
            SARS-CoV-2 RBD, elicits protective responses in animal models.
  JOURNAL   Mol Ther. 2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 
            10.1016/j.ymthe.2021.09.011.
  PUBMED    34547465
REFERENCE   2  (bases 1 to 9118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2021 Sep 20. pii: S1525-0016(21)00466-4. doi: 
            10.1016/j.ymthe.2021.09.011."
COMMENT     Sequence Label: pcDNA3.1_ spike_del19
FEATURES             Location/Qualifiers
     source          1..9118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     613..633
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4553..4570)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4559..4783
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4829..5257
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4916..4935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5126..5147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5266..5286)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5271..5600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5451..5586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5513..5532
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5667..6461
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5721..5740)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6331..6350
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6635..6756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6672..6691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6726..6745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6805..6821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6805..6821)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6818..6840)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6829..6845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6853..6859,6860..6877,6878..6883))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6898..6919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7036..7053)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7207..7795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7287..7306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7966..8757,8758..8826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8589..8608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8827..8931)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9006..9025)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
       61 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      721 atagggagac ccaagctggc tagcatgttt gtcttcctgg tcctgctgcc tctggtctcg
      781 tctcagtgcg tgaacctgac tactagaacc cagctgcctc ctgcctatac taactccttc
      841 acccgcggcg tgtactaccc agacaaggtg ttccgcagct ccgtgctgca ctccacccag
      901 gatctgttcc tgcccttctt cagcaacgtg acctggttcc acgccatcca cgtgagcggc
      961 accaatggca ccaagcggtt cgacaatccc gtgctgccat tcaacgatgg cgtgtacttc
     1021 gcctccaccg agaagagcaa catcatccgc ggctggatct tcggcaccac cctggactcc
     1081 aagacccaga gcctgctgat cgtgaacaat gccaccaacg tggtcatcaa ggtgtgcgag
     1141 ttccagttct gcaatgatcc attcctgggc gtgtactacc acaagaacaa taagtcctgg
     1201 atggagagcg agttccgcgt gtacagctcc gccaacaatt gcaccttcga gtacgtgtcc
     1261 cagcccttcc tgatggacct ggagggcaag cagggcaatt tcaagaacct gcgcgagttc
     1321 gtgttcaaga atatcgatgg ctacttcaag atctactcca agcacacccc catcaacctg
     1381 gtgcgcgacc tgccacaggg cttcagcgcc ctggagccac tggtggatct gccaatcggc
     1441 atcaacatca ccaggttcca gaccctgctg gccctgcacc gcagctacct gaccccaggc
     1501 gacagctcca gcggatggac cgctggagct gctgcctact acgtgggcta cctgcagccc
     1561 cgcaccttcc tgctgaagta caacgagaat ggcaccatca ccgacgccgt ggattgcgcc
     1621 ctggatccac tgtccgagac aaagtgcacc ctgaagagct tcaccgtgga gaagggcatc
     1681 taccagacct ccaatttccg cgtgcagcca accgagagca tcgtgcgctt ccccaatatc
     1741 accaacctgt gcccattcgg cgaggtgttc aacgctacca ggttcgccag cgtgtacgct
     1801 tggaatcgca agcgcatctc caactgcgtg gccgactaca gcgtgctgta caactccgcc
     1861 agcttctcca ccttcaagtg ctacggcgtg tcccccacca agctgaatga tctgtgcttc
     1921 accaacgtgt acgccgatag cttcgtgatc aggggcgacg aggtgcgcca gatcgctcca
     1981 ggacagaccg gcaagatcgc tgactacaat tacaagctgc ccgacgattt caccggctgc
     2041 gtgatcgcct ggaactccaa caatctggat agcaaagtgg gcggcaacta caattacctg
     2101 taccgcctgt tccgcaagtc caatctgaag ccattcgagc gcgacatctc caccgagatc
     2161 taccaggctg gaagcacccc atgcaatgga gtggagggct tcaactgcta cttccccctg
     2221 cagagctacg gcttccagcc aaccaacgga gtgggatacc agccatacag ggtggtggtg
     2281 ctgtccttcg agctgctgca cgctccagct accgtgtgcg gaccaaagaa gagcaccaat
     2341 ctggtgaaga acaagtgcgt gaacttcaat ttcaacggcc tgaccggaac cggcgtgctg
     2401 accgagtcca acaagaagtt cctgccattc cagcagttcg gaagggacat cgctgatacc
     2461 accgacgccg tgcgcgaccc acagaccctg gagatcctgg atatcacccc atgctccttc
     2521 ggcggcgtga gcgtgatcac cccaggaacc aataccagca accaggtggc cgtgctgtac
     2581 caggacgtga attgcaccga ggtgccagtg gctatccacg ctgatcagct gaccccaacc
     2641 tggcgcgtgt acagcaccgg atccaacgtg ttccagaccc gcgccggatg cctgatcgga
     2701 gctgagcacg tgaacaattc ctacgagtgc gacatcccaa tcggagctgg aatctgcgcc
     2761 agctaccaga cccagaccaa ctccccaagg agggctcgca gcgtggccag ccagtccatc
     2821 atcgcctaca ccatgtccct gggcgccgag aatagcgtgg cctacagcaa caattccatc
     2881 gccatcccaa ccaacttcac catctccgtg accaccgaga tcctgcccgt gtccatgacc
     2941 aagaccagcg tggactgcac catgtacatc tgcggcgatt ccaccgagtg cagcaacctg
     3001 ctgctgcagt acggcagctt ctgcacccag ctgaatcgcg ccctgaccgg aatcgctgtg
     3061 gagcaggata agaacaccca ggaggtgttc gcccaggtga agcagatcta caagaccccc
     3121 ccaatcaagg acttcggcgg cttcaatttc agccagatcc tgcccgatcc aagcaagccc
     3181 tccaagcgca gcttcatcga ggacctgctg ttcaacaagg tgaccctggc cgatgccggc
     3241 ttcatcaagc agtacggcga ttgcctgggc gacatcgctg cccgcgacct gatctgcgcc
     3301 cagaagttca atggcctgac cgtgctgcca ccactgctga ccgatgagat gatcgctcag
     3361 tacacctccg ccctgctggc cggaaccatc accagcggat ggaccttcgg cgctggagcc
     3421 gccctgcaga tccccttcgc catgcagatg gcctaccgct tcaacggcat cggcgtgacc
     3481 cagaatgtgc tgtacgagaa ccagaagctg atcgccaatc agttcaactc cgccatcggc
     3541 aagatccagg actccctgtc cagcaccgcc agcgccctgg gcaagctgca ggatgtggtg
     3601 aatcagaacg cccaggccct gaataccctg gtgaagcagc tgtccagcaa cttcggcgcc
     3661 atctccagcg tgctgaatga tatcctgagc cgcctggaca aggtggaggc tgaggtgcag
     3721 atcgataggc tgatcaccgg ccgcctgcag tccctgcaga cctacgtgac ccagcagctg
     3781 atcagggctg ctgagatcag ggccagcgcc aatctggctg ctaccaagat gtccgagtgc
     3841 gtgctgggac agagcaagag ggtggacttc tgcggcaagg gctaccacct gatgtccttc
     3901 ccacagagcg ccccacacgg agtggtgttc ctgcacgtga cctacgtgcc agcccaggag
     3961 aagaacttca ccaccgctcc agctatctgc cacgatggca aggctcactt cccacgcgag
     4021 ggcgtgttcg tgtccaacgg cacccactgg ttcgtgaccc agcgcaattt ctacgagccc
     4081 cagatcatca ccaccgacaa taccttcgtg agcggcaact gcgacgtggt catcggaatc
     4141 gtgaacaata ccgtgtacga tcccctgcag ccagagctgg actccttcaa ggaggagctg
     4201 gataagtact tcaagaatca caccagcccc gacgtggatc tgggcgacat ctccggcatc
     4261 aatgccagcg tggtgaacat ccagaaggag atcgaccgcc tgaacgaggt ggccaagaat
     4321 ctgaacgagt ccctgatcga tctgcaggag ctgggcaagt acgagcagta catcaagtgg
     4381 ccatggtaca tctggctggg cttcatcgcc ggcctgatcg ccatcgtgat ggtgaccatc
     4441 atgctgtgct gcatgacctc ctgctgcagc tgcctgaagg gctgctgctc ctgcggcagc
     4501 tgctgctagg aattcctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     4561 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4621 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4681 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4741 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4801 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5221 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5521 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5581 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5641 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5821 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     5881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6181 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6481 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6541 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6601 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6781 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6961 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7861 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7921 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7981 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8041 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8101 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8161 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8221 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8281 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8341 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8401 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8461 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8521 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8581 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8641 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8701 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8761 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8821 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8881 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8941 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9001 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9061 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttga
//
