LOCUS       sequence-300467-        9419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Patel M, Giddings AM, Sechelski J, Olsen JC
  TITLE     High efficiency gene transfer to airways of mice using influenza 
            hemagglutinin pseudotyped lentiviral vectors.
  JOURNAL   J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.
  PUBMED    23319179
REFERENCE   2  (bases 1 to 9419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jgm"; 
            journalName: "J Gene Med"; date: "2013-01"; volume: "15"; issue: 
            "1"; pages: "51-62"
COMMENT     Sequence Label: pNAHA
FEATURES             Location/Qualifiers
     source          1..9419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          692..1636
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1593..1613
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    3419..3540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3430..3449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3484..3503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3728..4315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4216..4235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4437..4628
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4460..4480)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          4835..5782
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     misc_feature    7257..7701
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        7735..7781
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7773..7791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7801..8826
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8838..9410
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catgatgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttgactag tcagggcccc
      421 aaccccccca agcccccatt tcacaacacg ctggcgctac aggcgcgtga cttccccttg
      481 ctttggggcg gggggctgag actcctatgt gctccggatt ggtcaggcac ggccttcggc
      541 cccgcctcct gccaccgcag attggccgct aggcctcccc gagcgccctg cctccgaggg
      601 ccggcgcacc ataaaagaag ccgccctagc cacgtcccct cgcagttcgg cggtcccgcg
      661 ggtctgtctc aagcttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      721 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccatgc ccctggctgc
      781 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
      841 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcttggg cgctggggcc
      901 gccgcgtgct aatctggtgg caccttcgcg cctgtctcgc tgctttcgct aagtctctag
      961 ccatttaaaa tttttgataa ccagctgcga cgcttttttt ctggcgagat agtcttgtaa
     1021 atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     1081 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     1141 tcggacgggg gtagtctcaa actggccggc ctgctctggt gcctggcctc gcgccgccgt
     1201 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     1261 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgcccgggag
     1321 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccttcctca tccgtcgctt
     1381 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttg tcgagctttt
     1441 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     1501 agtgggtgga gactgaagag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     1561 tgcccttttt gagtttggat cttgcctcat tctcaagcct cagacagtgg ttcaaagttt
     1621 ttttcttcca tttcaggtgt cgtgaaaact acccctaaaa gccggcgagc aaaagcaggg
     1681 gatacaaaat gaacactcaa atcctggttt tcgcccttgt ggcagtcatt cccacaaatg
     1741 cagacaaaat ttgtcttgga catcatgctg tatcaaatgg caccaaagta aacacactca
     1801 ctgagagagg agtagaagtt gtcaatgcaa cggaaacagt ggagcggaca aacatcccca
     1861 aaatttgctc aaaagggaaa agaaccactg atcttggcca atgcggactg ttagggacca
     1921 ttaccggacc acctcaatgc gaccaatttc tagaattttc agctgatcta ataatcgaga
     1981 gacgagaagg aaatgatgtt tgttacccgg ggaagtttgt taatgaagag gcattgcgac
     2041 aaatcctcag aggatcaggt gggattgaca aagaaacaat gggattcaca tatagtggaa
     2101 taaggaccaa cggaacaact agtgcatgta gaagatcagg gtcttcattc tatgcagaaa
     2161 tggagtggct cctgtcaaat acagacaatg cttctttccc acaaatgaca aaatcataca
     2221 aaaacacaag gagagaatca gctctgatag tatggggaat ccaccattca ggatcaacca
     2281 ccgaacagac caaactatat gggagtggaa ataaactgat aacagtcggg agttccaaat
     2341 atcatcaatc ttttgtgccg agtccaggaa cacgaccgca gataaatggc cagtccggac
     2401 ggattgattt tcattggttg atcttggatc ccaatgatac agttactttt agtttcaatg
     2461 gggctttcat agctccaaat cgtgccagct tcttgagggg aaagtccatg gggatccaga
     2521 gcgatgtgca ggttgatgcc aattgcgaag gggaatgcta ccacagtgga gggactataa
     2581 caagcagatt gccttttcaa aacatcaata gcagagcagt tggcaaatgc ccaagatatg
     2641 taaaacagga aagtttatta ttggcaactg ggatgaagaa cgttcccgaa ccttccaaaa
     2701 aaaggaaaaa aagaggcctg tttggcgcta tagcagggtt tattgaaaat ggttgggaag
     2761 gtctggtcga cgggtggtac ggtttcaggc atcagaatgc acaaggagaa ggaactgcag
     2821 cagactacaa aagcacccaa tcggcaattg atcagataac cggaaagtta aatagactca
     2881 ttgagaaaac caaccagcaa tttgagctaa tagataatga attcactgag gtggaaaagc
     2941 agattggcaa tttaattaac tggaccaaag actccatcac agaagtatgg tcttacaatg
     3001 ctgaacttat tgtggcaatg gaaaaccagc acactattga tttggctgat tcagagatga
     3061 acaggctgta tgagcgagtg aggaaacaat taagggaaaa tgctgaagag gatggcactg
     3121 gttgctttga aatttttcat aaatgtgacg atgattgtat ggctagtata aggaacaata
     3181 cttatgatca cagcaaatac agagaagaag cgatgcaaaa tagaatacaa attgacccag
     3241 tcaaattgag tagtggctac aaagatgtga tactttggtt tagcttcggg gcatcatgct
     3301 ttttgcttct tgccattgca atgggccttg ttttcatatg tgtgaagaac ggaaacatgc
     3361 ggtgcactat ttgtatataa gtttggaaaa acacccttgt ttctactgct agacatgata
     3421 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3541 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3601 taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta gccatgacca
     3661 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3721 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3781 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3841 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3901 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3961 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4021 cggataaggc gcagcggtcg ggctgaacgg gggttcgtgc acacagccca gcttggagcg
     4081 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4141 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4201 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4261 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4321 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4381 aattaacctg cagggcctga aataacctct gaaagaggaa cttggttagg taccttctga
     4441 ggctgaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4621 atagtcccac tagttccgcc agagcgcgcg agggcctcca gcggccgccc ctcccccaca
     4681 gcaggggcgg ggtcccgcgc ccaccggaag gagcgggctc ggggcgggcg gcgctgattg
     4741 gccggggcgg gcctgacgcc gacgcggcta taagagacca caagcgaccc gcagggccag
     4801 acgttcttcg ccgaagcttg ccgtcagaac gcaggtgagg ggcgggtgtg gcttccgcgg
     4861 gccgccgagc tggaggtcct gctccgagcg ggccgggccc cgctgtcgtc ggcggggatt
     4921 agctgcgagc attcccgctt cgagttgcgg gcggcgcggg aggcagagtg cgaggcctag
     4981 cggcaacccc gtagcctcgc ctcgtgtccg gcttgaggcc tagcgtggtg tccgcgccgc
     5041 cgccgcgtgc tactccggcc gcactctggt cttttttttt tttgttgttg ttgccctgct
     5101 gccttcgatt gccgttcagc aataggggct aacaaaggga gggtgcgggg cttgctcgcc
     5161 cggagcccgg agaggtcatg gttggggagg aatggaggga caggagtggc ggctggggcc
     5221 cgcccgcctt cggagcacat gtccgacgcc acctggatgg ggcgaggcct ggggtttttc
     5281 ccgaagcaac caggctgggg ttagcgtgcc gaggccatgt ggccccagca cccggcacga
     5341 tctggcttgg cggcgccgcg ttgccctgcc tccctaacta gggtgaggcc atcccgtccg
     5401 gcaccagttg cgtgcgtgga aagatggccg ctcccgggcc ctgttgcaag gagctcaaaa
     5461 tggaggacgc ggcagcccgg tggagcgggc gggtgagtca cccacacaaa ggaagagggc
     5521 ctggtccctc accggctgct gcttcctgtg accccgtggt cctatcggcc gcaatagtca
     5581 cctcgggctt ttgagcacgg ctagtcgcgg cggggggagg ggatgtaatg gcgttggagt
     5641 ttgttcacat ttggtgggtg gagactagtc aggccagcct ggcgctggaa gtcatttttg
     5701 gaatttgtcc ccttgagttt tgagcggagc taattctcgg gcttcttagc ggttcaaagg
     5761 tatcttttaa accctttttt aggtgttgtg aaaaccaccg ctaattcaaa gcaaccggtg
     5821 atatcggatc cacgcgtagc aaaagcaggg gtttaaaatg aatccaaatc agaaaataat
     5881 aaccattgga tcaatctgtc tggtagtcgg actaattagc ctaatattgc aaatagggaa
     5941 tataatctca atatggatta gccattcaat tcaaactgga agtcaaaacc atactggaat
     6001 atgcaaccaa aacatcattg cctataaaaa tagcacctgg gtaaaggaca caacttcagt
     6061 gatattaacc ggcaattcat ctctttgtcc catccgtggg tgggctatat acagcaaaga
     6121 caatagcata agaattggtt ccaaaggaga cgtttttgtc ataagagagc cctttatttc
     6181 atgttctcac ttggaatgca ggaccttttt tctgacccaa ggtgccttac tgaatgacaa
     6241 gcattcaagt gggactgtta aggacagaag cccttatagg gccttaatga gctgccctgt
     6301 cggtgaagct ccgtccccgt acaattcaag atttgaatcg gttgcttggt cagcaagtgc
     6361 atgtcatgat ggcatgggct ggctaacaat cggaatttca ggtccagata atggagcagt
     6421 ggctgtatta aaatacaacg gcataataac tgaaaccata aaaagttgga ggaagaaaat
     6481 attgaggaca caagagtctg aatgtgcctg tgtaaatggt tcatgtttta ctataatgac
     6541 tgatggcccg agtgatgggc tggcctcgta caaaattttc aagatcgaaa aggggaaggt
     6601 tactaaatca atagagttga atgcacctaa ttctcactat gaggaatgtt cctgttaccc
     6661 tgataccggc aaagtgatgt gtgtgtgcag agacaattgg catggttcga accggccatg
     6721 ggtgtctttc gatcaaaacc tggattatca aataggatac atctgcagtg gggttttcgg
     6781 tgacaacccg cgtcccgaag atggaacagg cagctgtggt ccagtgtatg ttgatggagc
     6841 aaacggagta aagggatttt catataggta tggtaatggt gtttggatag gaaggaccaa
     6901 aagtcacagt tccagacatg ggtttgagat gatttgggat cctaatggat ggacagagac
     6961 tgatagtaag ttctctgtga ggcaagatgt tgtggcaatg actgattggt cagggtatag
     7021 cggaagtttc gttcaacatc ctgagctgac agggctagac tgtatgaggc cgtgcttctg
     7081 ggttgaatta atcaggggac gacctaaaga aaaaacaatc tggactagtg cgagcagcat
     7141 ttctttttgt ggcgtgaata gtgatactgt agattggtct tggccagacg gtgctgagtt
     7201 gccattcagc attgacaagt agtctgttca aaaaactcct tgtttctact cctaggagca
     7261 ggtttcccca atgacacaaa acgtgcaact tgaaactccg cctggtcttt ccaggtctag
     7321 aggggtaaca ctttgtactg cgtttggctc cacgctcgat ccactggcga gtgttagtaa
     7381 cagcactgtt gcttcgtagc ggagcatgac ggccgtggga actcctcctt ggtaacaagg
     7441 acccacgggg ccaaaagcca cgcccacacg ggcccgtcat gtgtgcaacc ccagcacggc
     7501 gactttactg cgaaacccac tttaaagtga cattgaaact ggtacccaca cactggtgac
     7561 aggctaagga tgcccttcag gtaccccgag gtaacacgcg acactcggga tctgagaagg
     7621 ggactggggc ttctataaaa gcgctcggtt taaaaagctt ctatgcctga ataggtgacc
     7681 ggaggtcggc acctttcctt tgcaattact gaccctatga atacaactga ctgtttgaca
     7741 attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag gagggccacc
     7801 atgaagaaac ctgaactgac agcaacttct gttgagaagt ttctcattga aaaatttgat
     7861 tctgtttctg atctcatgca gctgtctgaa ggtgaagaaa gcagagcctt ttcttttgat
     7921 gttggaggaa gaggttatgt tctgagggtc aattcttgtg ctgatggttt ttacaaagac
     7981 agatatgttt acagacactt tgcctctgct gctctgccaa ttccagaagt tctggacatt
     8041 ggagaatttt ctgaatctct cacctactgc atcagcagaa gagcacaagg agtcactctc
     8101 caggatctcc ctgaaactga gctgccagct gttctgcaac ctgttgctga agcaatggat
     8161 gccattgcag cagctgatct gagccaaacc tctggatttg gtccttttgg tccccaaggc
     8221 attggtcagt acaccacttg gagggatttc atttgtgcca ttgctgatcc tcatgtctat
     8281 cactggcaga ctgtgatgga tgacacagtt tctgcttctg ttgctcaggc actggatgaa
     8341 ctcatgctgt gggcagaaga ttgtcctgaa gtcagacacc tggtccatgc tgattttgga
     8401 agcaacaatg ttctgacaga caatggcaga atcactgcag tcattgactg gtctgaagcc
     8461 atgtttggag attctcaata tgaggttgcc aacatttttt tttggagacc ttggctggct
     8521 tgcatggaac aacaaacaag atattttgaa agaagacacc cagaactggc tggttccccc
     8581 agactgagag cctacatgct cagaattggc ctggaccaac tgtatcaatc tctggttgat
     8641 ggaaactttg atgatgctgc ttgggcacaa ggaagatgtg atgccattgt gaggtctggt
     8701 gctggaactg ttggaagaac tcaaattgca agaaggtctg ctgctgtttg gactgatgga
     8761 tgtgttgaag ttctggctga ctctggaaac aggagaccct ccacaagacc cagagccaag
     8821 gaatgaatat tagctagatt atccctaata cctgccaccc cactcttaat cagtggtgga
     8881 agaacggtct cagaactgtt tgtttcaatt ggccatttaa gtttagtagt aaaagactgg
     8941 ttaatgataa caatgcatcg taaaaccttc agaaggaaag gagaatgttt tgtggaccac
     9001 tttggttttc ttttttgcgt gtggcagttt taagttatta gtttttaaaa tcagtacttt
     9061 ttaatggaaa caacttgacc aaaaatttgt cacagaattt tgagacccat taaaaaagtt
     9121 aaatgagaaa cctgtgtgtt cctttggtca acaccgagac atttaggtga aagacatcta
     9181 attctggttt tacgaatctg gaaacttctt gaaaatgtaa ttcttgagtt aacacttctg
     9241 ggtggagaat agggttgttt tccccccaca taattggaag gggaaggaat atcatttaaa
     9301 gctatgggag ggttgctttg attacaacac tggagagaaa tgcagcatgt tgctgattgc
     9361 ctgtcactaa aacaggccaa aaactgagtc cttgggttgc atagaaagct gcctgcagg
//
