LOCUS       sequence-300475-       12653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2,
            and N3 are removed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12653)
  AUTHORS   Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage
            DF
  TITLE     Multivalent interactions between CsoS2 and Rubisco mediate 
            alpha-carboxysome formation.
  JOURNAL   Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 
            10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
  PUBMED    32123388
REFERENCE   2  (bases 1 to 12653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-020-0387-7"; journalName: "Nat Struct Mol Biol"; 
            date: "2020-03"; volume: "27"; issue: "3"; pages: "281-287"
COMMENT     Sequence Label: pLz76_CB_operon_CsoS2_N4
FEATURES             Location/Qualifiers
     source          1..12653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      161..247
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      339..366
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     protein_bind    388..407
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        join(422..427,428..445,446..452)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    460..476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8406..9065
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(8472..8491)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        9130..9207
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             9208..10290
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(9227..9246)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      complement(11272..11860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11352..11371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggatgtagt agccgatcag gttcagctcg acttgaccag ctgcgctggc tgagacgatg
       61 cgaaccttct ccagcttcag ccgtggctcc cagcgatcga gcgcttcagc tgtggccgcc
      121 accaggtcaa cgatgaggga ctggttgatc ggctagtcat caaataaaac gaaaggctca
      181 gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      241 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
      301 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
      361 cctttttgcg tttctactct agtttcgaat tgtgagcgct cacaatttcg aaaccccagg
      421 cttgacactt tatgcttccg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc
      481 agctaggtgc gcgaatcccc catcctttca ggaggaactc atggcagtta aaaagtatag
      541 tgctggtgta aaagaatacc ggcagaccta ttggatgccg gaatacacac cgttggattc
      601 cgacatcctt gcatgcttca aaatcacccc acaaccgggt gttgatcgcg aagaagccgc
      661 agccgcggtt gcagcagaat cttcaaccgg cacatggacc accgtgtgga ccgatttgct
      721 gaccgacatg gactactaca aaggccgtgc ctatcgcatt gaagacgtac ccggtgacga
      781 tgcggcattc tatgccttta tcgcctaccc aatcgacctg ttcgaagaag ggtcagttgt
      841 taacgtgttt acctcactgg ttggtaacgt gttcggcttc aaagcggtac gcggcctgcg
      901 tctggaagat gttcgcttcc cactcgccta cgttaaaacc tgtggcggcc caccgcacgg
      961 tattcaagtc gaacgcgaca agatgaacaa atatggtcgc ccactgttgg gttgcaccat
     1021 caagccaaaa cttggtttgt ctgcgaaaaa ctacggccgt gccgtatacg agtgcctccg
     1081 tggcggcctc gacttcacta aagatgatga aaacatcaat tctcagccgt tcatgcgctg
     1141 gcgcgatcgc ttcttgttcg tacaagacgc gaccgaaact gctgaagccc aaaccggcga
     1201 acgcaaaggc cattacctca acgtaacggc gccaactcct gaagaaatgt acaagcgcgc
     1261 agaattcgcc aaagaaattg gcgcgccaat cattatgcac gactacatca ccggtggctt
     1321 cacggccaac actggcttgg ccaagtggtg tcaagacaac ggcgtactgc tgcacatcca
     1381 ccgtgcgatg catgcggtta tcgaccgtaa cccgaaccac ggtattcact tccgtgttct
     1441 gaccaagatt ctgcgtttgt cgggtggcga tcacctgcac accggtaccg ttgtcggcaa
     1501 actggaaggc gaccgtgcct ctactctggg ctggattgat ttgctccgcg aatcgtttat
     1561 ccctgaagat cgctcgcgcg gtatcttctt cgatcaagac tggggttcaa tgccaggcgt
     1621 attcgctgtg gcctctggtg gtattcacgt atggcacatg cctgcgctgg ttaacatctt
     1681 tggtgacgac tctgtcctcc aattcggtgg cggtacgctg ggtcatccat ggggcaacgc
     1741 tgccggtgct gctgccaacc gtgttgctct ggaagcctgc gtagaagcgc gtaaccaagg
     1801 ccgcgatatc gaaaaagaag gcaaagaaat tctgactgct gctgcacagc acagcccaga
     1861 actgaagatt gccatggaaa cttggaaaga gatcaaattc gaatttgaca ctgtcgacaa
     1921 actcgacact caaaatcgtt gatccctcgt accacacaac atactaaggt gagtaaccat
     1981 ggctgaaatg caggattaca agcaaagcct caaatatgag actttctctt atcttccacc
     2041 catgaacgcg gaacgcatcc gcgctcaaat caagtacgca attgctcaag gctggagccc
     2101 cggcattgag cacgtagaag tgaaaaactc catgaaccaa tattggtaca tgtggaaact
     2161 tcccttcttc ggcgaacaaa atgtcgacaa cgtgttggct gaaattgaag cgtgtcgtag
     2221 tgcgtatcca acacaccagg tcaaactggt ggcttatgac aactatgcgc aaagcttagg
     2281 tctggccttc gtggtctacc gcggcaacta agtcaagtct gtcattgcgc tgtgcgtttc
     2341 tctacgcaca gcacttattc aagatggggt caaacatgcg cgccgtgccg ccaaagccgc
     2401 aatctcaagg tggtccagga cgcaatggct atcaaattga tggataccta gataccgcac
     2461 ttcatggccg cgatgccgcc aagcgccacc gtgaaatgct ctgtcaatac ggccgcggca
     2521 cagcaccttc ctgcaagcca acaggccgtg tcaaaaattc tgtacagtcg ggcaacgcag
     2581 cgccaaaaaa ggttgaaacc ggtcacaccc tatccggcgg atctgttacg ggcacgcaag
     2641 tggatcgtaa atctcatgtg actggaaacg agccgggcac ttgccgagca gtcacgggca
     2701 ccgagtacgt aggtactgag caattcacct ctttttgcaa taccagcccc aagccaaatg
     2761 cgacgaaggt caatgtgacc acaacggctc gtggtcgccc tgttagcggt acggaagttt
     2821 cacggaccga gaaggtaact ggcaacgaat ccggtgtctg ccgtaacgtt accggcaccg
     2881 aatacatgag taatgaagct cacttttctc tatgtggaac agccgcaaag ccttcacaag
     2941 cggataaagt catgttcggc gccacagcac gaacgcatca agtggtcagt ggcagtgatg
     3001 aattcaggcc ctcttctgtt acgggtaacg aatcgggtgc aaaacgcaca attaccggct
     3061 cgcagtacgc agacgaaggt cttgcgcgac tcacgatcaa cggagcacct gcaaaagtag
     3121 ccagaaccca cacctttgcg ggctctgacg ttaccggcac ggaaatcggt cgctctactc
     3181 gcgtaactgg tgatgaaagc ggttcgtgtc gttcaatctc aggcaccgag tatctcagta
     3241 acgagcaatt ccaatccttt tgtgacacaa aacctcaacg cagcccgttc aaggttggcc
     3301 aagatcgcac gaacaagggt cagtctgtga ctggtaactt ggttgatcgt tccgaactgg
     3361 ttacaggtaa cgaaccaggt tcatgctctc gggttacagg ctctcagtat ggccaaagca
     3421 aaatctgcgg tggtggcgtg ggaaaagtgc gctcaatgcg cacccttcgc ggcacctcag
     3481 tatctggcca acagctagat catgccccaa agatgtccgg tgacgagcgc ggcgggtgca
     3541 tgcccgtcac cggtaatgag tactacggtc gtgaacattt cgaaccgttt tgtacgagca
     3601 ccccagagcc cgaagctcaa tcaactgaac aatcattgac ctgtgaagga caaattatta
     3661 gcggcacttc agttgacgcc agtgatttgg tcacaggaaa tgaaatcggt gaacagcaac
     3721 tcatcagcgg tgacgcctat gttggcgcgc agcagacagg ttgccttccc actagtccac
     3781 gcttcaacca aactggcaat gttcagtcaa tgggttttaa gaacaccaat cagccagaac
     3841 aaaactttgc accaggtgaa gtaatgccta ctgactttag tattcaaacc ccagctcgct
     3901 cggctcagaa tcgcattaca ggtaacgaca ttgcgccctc aggtcgcatt acaggccctg
     3961 gtatgctggc aaccggcttg attacaggaa cccccgaatt caggcacgct gcgcgcgagt
     4021 tggttggttc tccacaaccc atggcaatgg ccatggccaa ccgtaataag gctgctcaag
     4081 cacctgttgt gcagccagaa gtggttgcaa ctcaggaaaa gcctgagttg gtatgtgcac
     4141 caagaagcga tcaaatggat cgtgtgagtg gcgaaggcaa agaacgttgc cacatcactg
     4201 gcgatgactg gtcagttaac aagcacatca ccggtacagc cggtcaatgg gcgagtggtc
     4261 gcaacccttc catgcgcggt aatgcgcgtg tggtcgaaac cagcgcgttt gccaatcgca
     4321 atgtgccaaa acctgaaaag ccgggctcca agatcacggg cagtagtggt aatgacaccc
     4381 aaggtagtct gatcacttac tccggcggcg cgcgcggttg agcgttaagt aaagtgtaac
     4441 gatcatgaac acccgtaaca cacgaagcaa gcaacgcgca ccgtttggtg ttagctcatc
     4501 agtcaaacct cggcttgact tgattgagca agcaccaaac cctgcctatg accgccatcc
     4561 tgcttgcata acgttgcctg agcgtacctg ccggcacccg ttaactgacc ttgaagccaa
     4621 cgaacaactg ggtcgttgcg aggatagcgt caagaaccgt tttgatcgcg ttatcccttt
     4681 cttgcaagtt gttgctggca ttcctcttgg tctggattat gttacccgtg ttcaagaatt
     4741 agcccagtcg tcgcttggac atacgctgcc cgaagaatta ctcaaagata attggatcag
     4801 tgggcacaat ttaaaaggca tttttggcta cgcgactgct aaagcactaa cagcagccac
     4861 ggaacaattc agtcgtaaaa taatgtctga gaaggacgat tccgcatcgg ccattggctt
     4921 cttcctggat tgcgggttcc acgcagtgga cataagccct tgtgccgatg gtcgtctcaa
     4981 agggttactg ccttacatat tgcgtttgcc ccttacggca ttcacctatc gtaaagccta
     5041 cgcaggctcg atgttcgata ttgaagatga tctggcacag tgggagaaaa atgaactccg
     5101 ccgttatcgt gaaggtgttc caaatacagc ggatcagcca acacgatacc tgaaaattgc
     5161 tgtgtatcat ttcagcacct ctgacccgac acactctggc tgcgcggcac acggcagtaa
     5221 tgatcgtgca gcactggaag cggctttaac ccagctgatg aaattcagag aagcggttga
     5281 aaatgcccat tgctgcggcg caagtatcga tattttactg attggcgttg atacggatac
     5341 ggatgccatt cgcgttcata ttccggatag caaaggtttt ttgaatccgt atcgttatgt
     5401 tgacaacaca gtaacttatg cgcaaacact acatctggcg ccggatgagg ctcgtgtgat
     5461 tattcacgaa gcaattctca acgcaaaccg cagcgatggt tgggctaaag gaaatggagt
     5521 agccagcgag gggatgcgtc gttttattgg tcagcttttg atcaacaacc tctcgcaaat
     5581 cgattacgta gtaaatcgtc atggtggtcg ctatccaccc aatgatattg gtcatgctga
     5641 gcgatatatc agtgttggtg atggttttga tgaagttcaa atccggaatt tagcctacta
     5701 cgcgcatttg gatacggttg aagaaaatgc gattgatgtg gatgtgggaa tcaaaatttt
     5761 caccaaactt aatttgagtc gaggtttacc gattccgatt gccatccact atcgctatga
     5821 ccccaatgtt ccaggctcca gagaaagaac cgtggtcaaa gcaagacgga tatacaacgc
     5881 cattaaagag cggttctcat ccttggatga gcagaatcta ttgcagtttc gtttgagcgt
     5941 tcaggcgcag gatatcggaa gcccgattga agaggttgca tccgcatgaa aatcatgcaa
     6001 gttgagaaaa cgttggtttc aacaaaccgt attgctgata tgggtcacaa accactatta
     6061 gtggtatggg agaaaccggg cgcgcccagg caggtcgccg tggatgcgat tggctgcata
     6121 ccgggcgatt gggttttgtg cgttgggtca tcggcagcac gagaggctgc aggaagcaag
     6181 tcttatccct ctgatttgac gattatcggg attattgatc agtggaatgg tgagtaatgg
     6241 aagtaatgcg cgttcgttcc gacctaatcg caacacgcag gattcccggt cttaaaaata
     6301 tctctttgcg tgtcatggag gatgctacgg gtaaggtcag tgtcgcttgc gatcccattg
     6361 gcgttcccga gggatgttgg gtctttacga ttagcggctc tgccgctcgg tttggcgtgg
     6421 gtgattttga gattctcacg gatttgacga ttggtggcat catcgatcac tgggtaactt
     6481 gaccatcgct agatgagttg attttgaatg agtctttatt gaggagagaa gaaatggcag
     6541 cagtaacagg tattgcactg ggtatgattg aaacacgtgg tctggttcca gcgattgaag
     6601 ctgccgatgc catgaccaag gccgccgaag tacgtttggt tggccgtcaa tttgttggtg
     6661 gtggttacgt gaccgttttg gtccgtggtg aaaccggtgc cgtcaacgca gcagttcgtg
     6721 cgggcgctga tgcctgcgaa cgagtcggcg atggtctggt cgcggcgcat atcattgccc
     6781 gtgtccattc cgaagtcgaa aacatcctgc cgaaagcccc tgaagcttaa ggattgggaa
     6841 agacgaaccg gcgcaggctt gttccggttc tttgcataaa gtgacagctt aggagtttat
     6901 ttaaatggct gatgtaactg gtattgctct gggtatgatc gaaacacgtg gcttagttcc
     6961 tgcgattgaa gcagcggacg ccatgactaa agcggctgaa gtgcgtttgg tcggtcgtca
     7021 atttgttggt ggcggttacg tcaccgtatt ggttcggggc gaaacaggcg ctgttaacgc
     7081 cgctgttcgt gctggcgccg atgcttgcga acgtgttggt gatggcttgg ttgctgcgca
     7141 catcattgcg cgtgtccact cagaagtaga aaacattctg cctaaggcgc cacaagccta
     7201 agtcagatat tcctaagacg gctcacttcg gcgacacccg tcgccgaaac cgtctaacca
     7261 aatttctggg ctcgttctga acgtgctcaa cactagtttt agaggatctg ttatggcaac
     7321 gactcacggt attgccctgg gcatgattga aacacgagga ttggttcctg ccattgaagc
     7381 cgcagatgcc atgaccaaag cggcggaagt ccgtctggtc ggacgatcat ttgttggcgg
     7441 cggttacgtg accgtaatgg ttcgtggtga gacaggtgca gtaaatgctg ccgttcgtgc
     7501 gggtgctgac gcctgtgaac gtgttggcga tggcctggtt gctgcgcaca tcattgcgcg
     7561 cgttcattct gaagttgaga tcatcctacc cgagacgccc gaagactcag attccgcgtg
     7621 gtgtatcgca aatctgaata gctaatgtct agtagggaag atgcgcatga acaacattga
     7681 tttgcgcgtc tattcgttta tcgattcgct tcaaccgcag cttgcatcct atcttgcgac
     7741 atcatcgcaa ggctttcttc ccgttccggg tgatgcctgc ttgtggattg aagtcgcgcc
     7801 gggcatggcc gtccatcgcc tcagtgatat tgcgctaaag gccacgaacg ttcgtctcgg
     7861 cgaacaggta gtcgagcgtg cttttggctc gatggaaatt cattaccgaa accaaagcga
     7921 cgttctcgca tccggtgagg ccgttttaag agaaatcaat cacgcgcaag aagatcgtct
     7981 gccttgtcgc atcgcatgga aagagatcat tcgagcgatt acccccgatc atgccacctt
     8041 gatcaatcgc cagttgcgta aaggctctat gctgttgccg ggcaaaagca tgttcatcct
     8101 tgaaacagaa ccggcaggtt atattgttca ggctgccaac gaggccgaga aagcagctca
     8161 tgtgactctg atcgatgtac gtgcttttgg taactttggt cgcctgacca tgatgggcag
     8221 cgaagcggaa accgaagaag ccatgcgggc ggctgaggcc acaatcgcaa gcatcaacgc
     8281 gcgtgcgcgt cgcgctgaag ggttctaagc ggccgcgagg ttccaacttt caccataatg
     8341 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     8401 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     8461 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     8521 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     8581 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga gttccgtatg gcaatgaaag
     8641 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     8701 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     8761 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     8821 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     8881 acgtggccaa tatggacaac ttcttcgccc ccgttttcac tatgggcaaa tattatacgc
     8941 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     9001 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     9061 cgtaattttt ttaaggcagt tattggtgcc cttgaattcc tactagtcga agcggcatgc
     9121 atttacgttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg
     9181 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
     9241 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
     9301 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
     9361 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
     9421 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
     9481 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
     9541 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
     9601 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
     9661 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
     9721 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
     9781 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
     9841 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
     9901 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
     9961 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
    10021 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
    10081 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
    10141 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
    10201 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
    10261 caggtttccc gactggaaag cgggcagtga tcccacagcc gccagttccg ctggcggcat
    10321 tttaactttc tttaataatc tagtgacaag ccggggcaga cgtgagccgt agtcccgtcg
    10381 ccagacgcgg gtgcccacgg gcgtcgtcag gatgtccgta attgactgcc ggaggtggtc
    10441 aatgcccttc agctccttgc cactgtcacg gctcatgcct cgggtcatta gtcgcccgct
    10501 ccggtatctt cactggcttc gatgattgcc gccccgcagc tgcagaggtc accgatccga
    10561 gcagtcggcc tctggttggt aaagaccgtg cgactgccgg tgatgatcgt gttcaagcca
    10621 tgcagggggc aggcgtgaag gtcatccttt cgggccacgg ggcggctgtt gacgaaggtg
    10681 tcgtcgctcc cggtgatgat gatcccgccg tgatcggtca cgtcgtttag tcgagcgatg
    10741 cctggcgtcg tagtcacggg tttaggtcaa tacgacttgc ggtcactgta acgttgccct
    10801 cggcggtcac gttaacgtcg ccttgggctt cgacttgcgc ctcctgcaca aggatcacaa
    10861 tccgtccttg ggctgcggtg aggtcgatct tgtactcatg cgcttcgcgg tcgtactgga
    10921 tgattgagtc atcctcgaac tgcgtctttt ggatcgtttc tttgtcctcg atctgggggt
    10981 agtcagtcga gaacgcgccg ggcatcgcga agccctgact gatctcgccg gagggggcca
    11041 tcacgacgac ggcctcaccg acctcgggcg cccaccagaa ccgatccttg cccgctcgct
    11101 gcgtgagcca cggaatccag tcagtgagga gcagcgcctc gccgctctcc tcgtcttcct
    11161 cgatcgcgac acggatcagc cccttgggat agtcagcctc ggctaccctg cctacgcgga
    11221 gcaagttgcc gtgacgccga ctgtctcgag tctagtcgcg ttgctggcgt ttttccatag
    11281 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11341 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11401 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11461 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11521 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11581 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11641 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11701 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    11761 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    11821 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    11881 tacggggtct gacgctcagt ctagttcagc cagctcgtcg tgatgtcgaa acccaagcca
    11941 ccctcagagg tgaaggccgc ttcgagcaca tcgggaagcg tgtcgacgac ggtgctcggt
    12001 gcctcgggta agagccagat agatgcggtg acgttcaccg tcgcgatcgt ggcgggaacc
    12061 acggtcactg cgtccgttac aactcgcacg tcgtcggcca gcacgactga ctcgacggct
    12121 tcgattagcc cgggagaggc aaggccatca ggctcggtag acagaatgct gattaacacc
    12181 tcgccgggag cagggctgct cacagccgcg tccttcaccc gtgggtcagc cgtcagcgct
    12241 tgatagcggt accaggccgc accgcctgcg gtcgagctgc ccttgatccg ctcgatggtg
    12301 cgatcgcgaa gctcgccatc cggctcattc ggctgccgca ccacgccgta gaacgcggca
    12361 aggttgtcga ggtcggggcc gtttgagtag cgaagcagtg tcgcaagaag tgcgtcgttg
    12421 atccgctgac gcaggatcag ctcgcgggct gcgcagacct ccagcagctt gatgaccggg
    12481 tcggattcga ggatggcggt gtagctggcg tcacgcgatc gcaggtcgtc gatcaagtcc
    12541 tgcaggatca gttcaaagtc cagcgcttcg atgatggtgg gcgcgggaat cgtagcaaag
    12601 tcaagaacgg tcatgagacg actaagccct ccagcgtgat acgcctgccc tcg
//
