LOCUS       sequence-300498-        6576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6576)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4252982
FEATURES             Location/Qualifiers
     source          1..6576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        670..1879
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1716..1735
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1883..2911
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     polyA_signal    2938..2986
                     /note="synthetic polyadenylation signal"
     CDS             join(3252..3320,3321..4112)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3470..3489)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      4125..4261
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4413..4484
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4489..4535)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4553..4601
                     /note="synthetic polyadenylation signal"
     enhancer        4682..4985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4987..5189
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5140..5160
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5258..5267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5368..5489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5379..5398)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5433..5452
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      5604..5650
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      5680..5816
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5861..5932
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(join(6158..6576,1..170))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6238..6257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
       61 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      121 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      181 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      241 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      301 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      361 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcattt aaatttccga
      421 actctccaag gccctcgtcg gaaaatcttc aaacctttcg tccgatccat cttgcaggct
      481 acctctcgaa cgaactatcg caagtctctt ggccggcctt gcgccttggc tattgcttgg
      541 cagcgcctat cgccaggtat tactccaatc ccgaatatcc gagatcggga tcacccgaga
      601 gaagttcaac ctacatcctc aatcccgatc tatccgagat ccgaggaata tcgaaatcgg
      661 ggcgcgcctg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcgtcacgg
      721 cgagcgctgc cacgtcagac gaagggcgca ggagcgtcct gatccttccg cccggacgct
      781 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      841 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      901 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      961 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     1021 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     1081 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     1141 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     1201 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     1261 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     1321 gagcccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg caccatctgg
     1381 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     1441 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     1501 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc accagccggt
     1561 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     1621 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     1681 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     1741 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     1801 tgggtcaata tgtaattttc agtgttagac ttgtaaattg tccgctaaat tctggccgtt
     1861 tttggctttt ttgttagaca acatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga
     1921 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
     1981 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
     2041 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat ccgccgcgct
     2101 cccgattccg gaagtgcttg acattgggga gttcagcgag agcctgacct attgcatctc
     2161 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
     2221 gcagccggtc gcggaggcaa tggatgccat cgctgccgcc gatcttagcc agacgagcgg
     2281 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
     2341 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
     2401 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
     2461 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
     2521 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
     2581 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
     2641 gcatccggag cttgcaggat cgccccggct ccgggcgtat atgctccgca ttggtcttga
     2701 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
     2761 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
     2821 aagcgccgcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
     2881 ccccagcact cgtccgaggg caaaggaata agctagtatg taagcctagt cttagataat
     2941 aaaatcgcta tccatcgaag atggatgtgt gttggttttt tgtgtgtgta acgctaggcg
     3001 cgcctggtgt accgagaacg atcctctcag tgcgagtctc gacgatccat atcgttgctt
     3061 ggcagtcagc cagtcggaat ccagcttggg acccaggaag tccaatcgtc agatattgta
     3121 ctcaagcctg gtcacggcag cgtaccgatc tgtttaaacc tagatattga tagtctgatc
     3181 ggtcaacgta taatcgagtc ctagcttttg caaacatcta tcaagagaca ggatcagcag
     3241 gaggctttcg catgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3301 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3361 agttgggtgc gcgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     3421 gttttcgccc cgaagaacgc tttccaatga tgagcacttt taaagttctg ctatgtggcg
     3481 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     3541 agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag
     3601 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     3661 tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     3721 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     3781 acaccacgat gcctgtagca atggcaacaa ccttgcgtaa actattaact ggcgaactac
     3841 ttactctagc ttcccggcaa cagttgatag actggatgga ggcggataaa gttgcaggac
     3901 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     3961 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4021 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4081 agataggtgc ctcactgatt aagcattggt aaccgattct aggtgcattg gcgcagaaaa
     4141 aaatgcctga tgcgacgctg cgcgtcttat actcccacat atgccagatt cagcaacgga
     4201 tacggcttcc ccaacttgcc cacttccata cgtgtcctcc ttaccagaaa tttatcctta
     4261 agatcccgaa tcgtttaaac tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta
     4321 cgcgaacagc cgtggcgctc atttgctcgt cgggcatcga atctcgtcag ctatcgtcag
     4381 cttacctttt tggcagcgat cgcggctccc gacatcttgg accattagct ccacaggtat
     4441 cttcttccct ctagtggtca taacagcagc ttcagctacc tctcaattca aaaaacccct
     4501 caagacccgt ttagaggccc caaggggtta tgctatcaat cgttgcgtta cacacacaaa
     4561 aaaccaacac acatccatct tcgatggata gcgattttat tatctaactg ctgatcgagt
     4621 gtagccagat ctagtaatca attacggggt cattagttca tagcccatat atggagttcc
     4681 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     4741 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     4801 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     4861 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     4921 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4981 ccatgctgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     5041 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     5101 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     5161 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc agatctttgt
     5221 cgatcctacc atccactcga cacacccgcc agcggccgcc accatggctg atgcacaatc
     5281 gtttttaaac gggtttgcgt aaggatccgc aggcctctgc tagcttgact gactgagata
     5341 cagcgtacct tcagctcaca gacatgataa gatacattga tgagtttgga caaaccacaa
     5401 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5461 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5521 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     5581 ttggcccatc tctatcggta tcgtagcata accccttggg gcctctaaac gggtcttgag
     5641 gggttttttg tgcccctcgg gccggattgc tatctaccgg cattggcgca gaaaaaaatg
     5701 cctgatgcga cgctgcgcgt cttatactcc cacatatgcc agattcagca acggatacgg
     5761 cttccccaac ttgcccactt ccatacgtgt cctccttacc agaaatttat ccttaaggtc
     5821 gtcagctatc ctgcaggcga tctctcgatt tcgatcaaga cattccttta atggtctttt
     5881 ctggacacca ctaggggtca gaagtagttc atcaaacttt cttccctccc taatctcatt
     5941 ggttaccttg ggctatcgaa acttaattaa ccagtcaagt cagctacttg gcgagatcga
     6001 cttgtctggg tttcgactac gctcagaatt gcgtcagtca agttcgatct ggtccttgct
     6061 attgcacccg ttctccgatt acgagtttca tttaaatcat gtgagcaaaa ggccagcaaa
     6121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6541 atgtaggcgg tgctacagag ttcttgaagt ggtggc
//
