LOCUS       sequence-300500-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 ORF7b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4136068
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    33..154
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(70..89)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     124..143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(410..612)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(439..459)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        614..917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    998..1046
                     /note="synthetic polyadenylation signal"
     terminator      1064..1110
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1115..1186
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1338..1474)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1487..2278,2279..2347))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2110..2129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3093..3681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3582..3601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3907..3978
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4023..4159)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(4189..4235)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
       61 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
      121 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtgagctg aaggtacgct
      181 gtatctcagt cagtcaagct agcttaggcg tgacaagttt cattatgatc ttgcagttca
      241 agtgagaacc aaaagataat aagcataatt aaaacaagga atagcagaaa ggctaaaaag
      301 cacaaataga agtcaattaa tgaaagttca atcatggtgg cggccgctgg cgggtgtgtc
      361 gagtggatgg taggatcgac aaagatctga tctgacggtt cactaaacca gctctgctta
      421 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
      481 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
      541 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
      601 aaaaccgcat cagcatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
      661 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
      721 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
      781 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
      841 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
      901 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
      961 attactagat ctggctacac tcgatcagca gttagataat aaaatcgcta tccatcgaag
     1021 atggatgtgt gttggttttt tgtgtgtgta acgcaacgat tgatagcata accccttggg
     1081 gcctctaaac gggtcttgag gggttttttg aattgagagg tagctgaagc tgctgttatg
     1141 accactagag ggaagaagat acctgtggag ctaatggtcc aagatgtcgg gagccgcgat
     1201 cgctgccaaa aaggtaagct gacgatagct gacgagattc gatgcccgac gagcaaatga
     1261 gcgccacggc tgttcgcgta agctcgaaac gacggagatt cgatagagcc agagtcgagt
     1321 ttaaacgatt cgggatctta aggataaatt tctggtaagg aggacacgta tggaagtggg
     1381 caagttgggg aagccgtatc cgttgctgaa tctggcatat gtgggagtat aagacgcgca
     1441 gcgtcgcatc aggcattttt ttctgcgcca atgcacctag aatcggttac caatgcttaa
     1501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1621 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct atcaactgtt
     1741 gccgggaagc tagagtaagt agttcgccag ttaatagttt acgcaaggtt gttgccattg
     1801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1921 gtcctccaat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgaat
     2041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaagc
     2161 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     2221 ccactcgcgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     2281 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     2341 tactcatgcg aaagcctcct gctgatcctg tctcttgata gatgtttgca aaagctagga
     2401 ctcgattata cgttgaccga tcagactatc aatatctagg tttaaacaga tcggtacgct
     2461 gccgtgacca ggcttgagta caatatctga cgattggact tcctgggtcc caagctggat
     2521 tccgactggc tgactgccaa gcaacgatat ggatcgtcga gactcgcact gagaggatcg
     2581 ttctcggtac accaggcgcg ccccgatttc gatattcctc ggatctcgga tagatcggga
     2641 ttgaggatgt aggttgaact tctctcgggt gatcccgatc tcggatattc gggattggag
     2701 taatacctgg cgataggcgc tgccaagcaa tagccaaggc gcaaggccgg ccaagagact
     2761 tgcgatagtt cgttcgagag gtagcctgca agatggatcg gacgaaaggt ttgaagattt
     2821 tccgacgagg gccttggaga gttcggaaat ttaaatgcaa ctatggatga acgaaataga
     2881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2941 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3001 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3061 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3121 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3181 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3241 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3301 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3361 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3421 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3481 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3541 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3601 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3661 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3721 tgctggcctt ttgctcacat gatttaaatg aaactcgtaa tcggagaacg ggtgcaatag
     3781 caaggaccag atcgaacttg actgacgcaa ttctgagcgt agtcgaaacc cagacaagtc
     3841 gatctcgcca agtagctgac ttgactggtt aattaagttt cgatagccca aggtaaccaa
     3901 tgagattagg gagggaagaa agtttgatga actacttctg acccctagtg gtgtccagaa
     3961 aagaccatta aaggaatgtc ttgatcgaaa tcgagagatc gcctgcagga tagctgacga
     4021 ccttaaggat aaatttctgg taaggaggac acgtatggaa gtgggcaagt tggggaagcc
     4081 gtatccgttg ctgaatctgg catatgtggg agtataagac gcgcagcgtc gcatcaggca
     4141 tttttttctg cgccaatgcc ggtagatagc aatccggccc gaggggcaca aaaaacccct
     4201 caagacccgt ttagaggccc caaggggtta tgctacgata ccgatagaga tgggccaata
     4261 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacc
//
